Graf GTPase regulator associated with FAK [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Grafprovided by FlyBase
Official Full Name: GTPase regulator associated with FAKprovided by FlyBase
Primary source: FLYBASE:FBgn0030685
Locus tag: Dmel_CG8948
See related: AllianceGenome:FB:FBgn0030685
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG8948; D-Graf; Dmel\CG8948; graf; Q9VXQ2_DROME
Summary: Enables GTPase activator activity and ubiquitin-modified protein reader activity. Involved in several processes, including cytokinetic process; positive regulation of receptor internalization; and syncytial embryo cellularization. Located in several cellular components, including actomyosin contractile ring actin filament; cellularization cleavage furrow invagination front; and cytosol. Is expressed in adult head; leg; primary tracheal branch; spermatozoon; and tarsal segment. Used to study X-linked mental retardation with cerebellar hypoplasia and distinctive facial appearance. Human ortholog(s) of this gene implicated in X-linked mental retardation with cerebellar hypoplasia and distinctive facial appearance; acute myelomonocytic leukemia; and juvenile myelomonocytic leukemia. Orthologous to several human genes including ARHGAP26 (Rho GTPase activating protein 26). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 13E14-13E14; 1-51 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (15751674..15760733, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (15645707..15654766, complement)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Drosophila Graf regulates mushroom body beta-axon extension and olfactory long-term memory.
Title: Drosophila Graf regulates mushroom body β-axon extension and olfactory long-term memory.
Spatiotemporal recruitment of RhoGTPase protein GRAF inhibits actomyosin ring constriction in Drosophila cellularization.
Title: Spatiotemporal recruitment of RhoGTPase protein GRAF inhibits actomyosin ring constriction in Drosophila cellularization.
Graf functions to downregulate EGFR signaling.
Title: Graf regulates hematopoiesis through GEEC endocytosis of EGFR.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables GTPase activator activity	ISM Inferred from Sequence Model more info
enables phospholipid binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin-modified protein reader activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	ISM Inferred from Sequence Model more info
involved_in cleavage furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of epidermal growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of receptor internalization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actomyosin contractile ring contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in syncytial embryo cellularization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actomyosin contractile ring actin filament	IDA Inferred from Direct Assay more info	PubMed
located_in cellularization cleavage furrow invagination front	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: GTPase regulator associated with FAK

Names: CG8948-PA; CG8948-PD; CG8948-PE; CG8948-PG; CG8948-PH; CG8948-PI; CG8948-PJ; GRAF ortholog; GTPase regulator associated with focal adhesion kinase ortholog; Graf-PA; Graf-PD; Graf-PE; Graf-PG; Graf-PH; Graf-PI; Graf-PJ

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

15751674..15760733 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_206722.2 → NP_996445.1 GTPase regulator associated with FAK, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996445.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VXQ2

Conserved Domains (5) summary

cd07602
Location:27 → 233

BAR_RhoGAP_OPHN1-like; The Bin/Amphiphysin/Rvs (BAR) domain of Oligophrenin1-like Rho GTPase Activating Proteins

cd11882
Location:958 → 1011

SH3_GRAF-like; Src Homology 3 domain of GTPase Regulator Associated with Focal adhesion kinase and similar proteins

cd01249
Location:273 → 381

BAR-PH_GRAF_family; GTPase Regulator Associated with Focal adhesion and related proteins Pleckstrin homology (PH) domain

pfam00169
Location:273 → 374

PH; PH domain

cl02570
Location:374 → 575

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...
NM_132842.3 → NP_573070.2 GTPase regulator associated with FAK, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_573070.2

Status: REVIEWED

UniProtKB/TrEMBL

Q9VXQ2

Related

FBpp0073979

Conserved Domains (5) summary

cd07602
Location:27 → 233

BAR_RhoGAP_OPHN1-like; The Bin/Amphiphysin/Rvs (BAR) domain of Oligophrenin1-like Rho GTPase Activating Proteins

cd11882
Location:958 → 1011

SH3_GRAF-like; Src Homology 3 domain of GTPase Regulator Associated with Focal adhesion kinase and similar proteins

cd01249
Location:273 → 381

BAR-PH_GRAF_family; GTPase Regulator Associated with Focal adhesion and related proteins Pleckstrin homology (PH) domain

pfam00169
Location:273 → 374

PH; PH domain

cl02570
Location:374 → 575

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...
NM_001298367.1 → NP_001285296.1 GTPase regulator associated with FAK, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285296.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86NY4, Q9VXQ2, X2JDY8

Conserved Domains (4) summary

cd07602
Location:27 → 233

BAR_RhoGAP_OPHN1-like; The Bin/Amphiphysin/Rvs (BAR) domain of Oligophrenin1-like Rho GTPase Activating Proteins

cd11882
Location:967 → 1020

SH3_GRAF-like; Src Homology 3 domain of GTPase Regulator Associated with Focal adhesion kinase and similar proteins

cd01249
Location:273 → 390

BAR-PH_GRAF_family; GTPase Regulator Associated with Focal adhesion and related proteins Pleckstrin homology (PH) domain

cl02570
Location:383 → 584

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...
NM_206720.2 → NP_996443.1 GTPase regulator associated with FAK, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996443.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VXQ2

Conserved Domains (5) summary

cd07602
Location:27 → 233

BAR_RhoGAP_OPHN1-like; The Bin/Amphiphysin/Rvs (BAR) domain of Oligophrenin1-like Rho GTPase Activating Proteins

cd11882
Location:958 → 1011

SH3_GRAF-like; Src Homology 3 domain of GTPase Regulator Associated with Focal adhesion kinase and similar proteins

cd01249
Location:273 → 381

BAR-PH_GRAF_family; GTPase Regulator Associated with Focal adhesion and related proteins Pleckstrin homology (PH) domain

pfam00169
Location:273 → 374

PH; PH domain

cl02570
Location:374 → 575

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...
NM_206718.2 → NP_996441.2 GTPase regulator associated with FAK, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996441.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86NY4, Q9VXQ2, X2JDY8

Conserved Domains (4) summary

cd07602
Location:27 → 233

BAR_RhoGAP_OPHN1-like; The Bin/Amphiphysin/Rvs (BAR) domain of Oligophrenin1-like Rho GTPase Activating Proteins

cd11882
Location:967 → 1020

SH3_GRAF-like; Src Homology 3 domain of GTPase Regulator Associated with Focal adhesion kinase and similar proteins

cd01249
Location:273 → 390

BAR-PH_GRAF_family; GTPase Regulator Associated with Focal adhesion and related proteins Pleckstrin homology (PH) domain

cl02570
Location:383 → 584

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...
NM_206719.2 → NP_996442.1 GTPase regulator associated with FAK, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996442.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VXQ2

Conserved Domains (5) summary

cd07602
Location:27 → 233

BAR_RhoGAP_OPHN1-like; The Bin/Amphiphysin/Rvs (BAR) domain of Oligophrenin1-like Rho GTPase Activating Proteins

cd11882
Location:958 → 1011

SH3_GRAF-like; Src Homology 3 domain of GTPase Regulator Associated with Focal adhesion kinase and similar proteins

cd01249
Location:273 → 381

BAR-PH_GRAF_family; GTPase Regulator Associated with Focal adhesion and related proteins Pleckstrin homology (PH) domain

pfam00169
Location:273 → 374

PH; PH domain

cl02570
Location:374 → 575

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...
NM_206721.2 → NP_996444.2 GTPase regulator associated with FAK, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_996444.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86NY4, Q9VXQ2, X2JDY8

Conserved Domains (4) summary

cd07602
Location:27 → 233

BAR_RhoGAP_OPHN1-like; The Bin/Amphiphysin/Rvs (BAR) domain of Oligophrenin1-like Rho GTPase Activating Proteins

cd11882
Location:967 → 1020

SH3_GRAF-like; Src Homology 3 domain of GTPase Regulator Associated with Focal adhesion kinase and similar proteins

cd01249
Location:273 → 390

BAR-PH_GRAF_family; GTPase Regulator Associated with Focal adhesion and related proteins Pleckstrin homology (PH) domain

cl02570
Location:383 → 584

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...