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Top3alpha Topoisomerase 3alpha [ Drosophila melanogaster (fruit fly) ]

Gene ID: 35236, updated on 9-Dec-2024

Summary

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Official Symbol
Top3alphaprovided by FlyBase
Official Full Name
Topoisomerase 3alphaprovided by FlyBase
Primary source
FLYBASE:FBgn0040268
Locus tag
Dmel_CG10123
See related
AllianceGenome:FB:FBgn0040268
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG10123; Dmel\CG10123; DmTop3alpha; dTopoIIIa; top3a; Top3a; top3alpha; topo IIIalpha; Topo IIIalpha
Summary
Enables DNA topoisomerase activity. Involved in double-strand break repair via homologous recombination; mitochondrial genome maintenance; and tissue regeneration. Located in mitochondrial nucleoid and nucleus. Human ortholog(s) of this gene implicated in autosomal recessive progressive external ophthalmoplegia with mitochondrial DNA deletions 5. Orthologous to human TOP3A (DNA topoisomerase III alpha). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs
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Genomic context

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See Top3alpha in Genome Data Viewer
Location:
37E4-37E4; 2-54 cM
Exon count:
4
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2L NT_033779.5 (19498168..19502268)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2L NT_033779.4 (19498168..19502268)

Chromosome 2L - NT_033779.5Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene Cytosolic thiouridylase subunit 2 Neighboring gene second mitotic wave missing Neighboring gene cysts Neighboring gene Hig-anchoring scaffold protein

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Essential functions of C terminus of Drosophila Topoisomerase IIIα in double holliday junction dissolution. Chen SH, et al. J Biol Chem, 2012 Jun 1. PMID 22511792, Free PMC Article
  2. Drosophila topo IIIalpha is required for the maintenance of mitochondrial genome and male germ-line stem cells. Wu J, et al. Proc Natl Acad Sci U S A, 2010 Apr 6. PMID 20308575, Free PMC Article
  3. Bloom syndrome helicase in meiosis: Pro-crossover functions of an anti-crossover protein. Hatkevich T, et al. Bioessays, 2017 Sep. PMID 28792069, Free PMC Article
  4. Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging. Jiang YF, et al. Sci Rep, 2017 Mar 30. PMID 28358017, Free PMC Article
  5. Cloning and characterization of Drosophila topoisomerase IIIbeta. Relaxation of hypernegatively supercoiled DNA. Wilson TM, et al. J Biol Chem, 2000 Jan 21. PMID 10636841

See all (47) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The results reported here demonstrate that mitochondrial Top3alpha ablation results in mitochondrial genome instability and its dysfunction, thereby accelerating the aging process.
    Title: Drosophila mitochondrial topoisomerase III alpha affects the aging process via maintenance of mitochondrial function and genome integrity.
  2. the Top3alpha C terminus has an important role in dHJ dissolution (by providing an interaction interface for Blm and DNA) and an essential function in vivo.
    Title: Essential functions of C terminus of Drosophila Topoisomerase IIIα in double holliday junction dissolution.
  3. described the phenotypes associated with mitochondrial dysfunction, including the female sterility and the loss of germ-line stem cells of males, as a result from topo IIIalpha deprivation.
    Title: Drosophila topo IIIalpha is required for the maintenance of mitochondrial genome and male germ-line stem cells.
  4. partially relaxes a hypernegatively supercoiled plasmid substrate consistent with other purified topo IIIs.
    Title: Drosophila melanogaster topoisomerase IIIalpha preferentially relaxes a positively or negatively supercoiled bubble substrate and is essential during development.
  5. Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration
    Title: Topoisomerase IIIalpha and Bloom's helicase can resolve a mobile double Holliday junction substrate through convergent branch migration.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA topoisomerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA recombination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA topological change IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA topological change IEA
Inferred from Electronic Annotation
more info
  
involved_in developmental growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in double-strand break repair via homologous recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitochondrial genome maintenance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tissue regeneration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of RecQ family helicase-topoisomerase III complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrial nucleoid IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
topoisomerase 3alpha
Names
CG10123-PA
Top 3alpha
Top3alpha-PA
Topoisomerase3alpha
topoisomerase 3-alpha
topoisomerase IIIa
topoisomerase IIIalpha
NP_523602.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033779.5 Reference assembly

    Range
    19498168..19502268
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_078878.3 → NP_523602.2  topoisomerase 3alpha [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_523602.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q6AWG6, Q9NG98, Q9VIV1
    Related
    FBpp0080787
    Conserved Domains (6) summary
    cd03362
    Location:27 → 185
    TOPRIM_TopoIA_TopoIII; TOPRIM_TopoIA_TopoIII: The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving ...
    COG0550
    Location:27 → 618
    TopA; DNA topoisomerase IA [Replication, recombination and repair]
    COG0551
    Location:658 → 744
    YrdD; ssDNA-binding Zn-finger and Zn-ribbon domains of topoisomerase 1 [Replication, recombination and repair]
    cd00186
    Location:194 → 608
    TOP1Ac; DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA ...
    pfam01396
    Location:657 → 694
    zf-C4_Topoisom; Topoisomerase DNA binding C4 zinc finger
    pfam06839
    Location:1150 → 1193
    zf-GRF; GRF zinc finger
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9NG98.2 Q9NG98

Gene LinkOut

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