E2f2 E2F transcription factor 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: E2f2provided by FlyBase
Official Full Name: E2F transcription factor 2provided by FlyBase
Primary source: FLYBASE:FBgn0024371
Locus tag: Dmel_CG1071
See related: AllianceGenome:FB:FBgn0024371
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG1071; dE2F-2; de2f2; dE2f2; dE2F2; Dmel\CG1071; E2f; E2F; e2f2; E2F2
Summary: Enables RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Contributes to DNA-binding transcription repressor activity, RNA polymerase II-specific. Involved in several processes, including DNA endoreduplication; negative regulation of G1/S transition of mitotic cell cycle; and negative regulation of nucleobase-containing compound metabolic process. Located in chromatin. Part of DRM complex; Myb complex; and Rb-E2F complex. Is active in nucleus. Is expressed in several structures, including Malpighian tubule primordium; embryonic/larval midgut primordium; ganglia; gut section; and mesoderm. Human ortholog(s) of this gene implicated in prostate carcinoma. Orthologous to human E2F5 (E2F transcription factor 5). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 39A5-39A6; 2-54 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (21154293..21156749, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (21154293..21156749, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

E2F directly regulates the expression of late myogenic genes, with E2f1 providing the major contribution compared with E2f2.
Title: E2F function in muscle growth is necessary and sufficient for viability in Drosophila.
The dE2F2 protein repress the expression of buffy, the anti-apoptotic member of the Bcl-2 family and activate the expression of an RNA-binding protein that destabilizes diap1 in Drosophila.
Title: The pro-apoptotic activity of Drosophila Rbf1 involves dE2F2-dependent downregulation of diap1 and buffy mRNA.
Heterozygous mutation of mip120 or E2f2 suppressed the binucleate cell phenotype in the peripodial epithelium of Myb-null wing discs.
Title: The complex containing Drosophila Myb and RB/E2F2 regulates cytokinesis in a histone H2Av-dependent manner.
dE2F2, as well as the net E2F activity, which can be depleted by mutating the common cofactor, dDp, is inhibitory for p53-independent apoptosis.
Title: E2F1 and E2F2 have opposite effects on radiation-induced p53-independent apoptosis in Drosophila.
Combined inactivation of dE2F1 and dE2F2 had no significant effect on cell division of Hippo pathway mutant cells.
Title: Context-dependent requirement for dE2F during oncogenic proliferation.
Thease results demonstrate epigenetic regulation of gene expression by Myb, and E2F2-RBF in vivo, and also provide an explanation for the ability of Mip130-null mutants to rescue the lethality of Myb-null mutants in vivo.
Title: Epigenetic regulation of gene expression by Drosophila Myb and E2F2-RBF via the Myb-MuvB/dREAM complex.
cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression
Title: G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression.
examination of linking together the Myb and E2F2 complexes in higher-order assembly to specific chromosomal sites for the regulation of transcription
Title: Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex.
RBF1 and RBF2 interact with different subsets of E2F proteins; this enables the RBF proteins to regulate E2F-dependent transcription in distinct ways
Title: Distinct mechanisms of E2F regulation by Drosophila RBF1 and RBF2.
RBF2 has a unique function in repressing E2F-regulated differentiation markers and dE2F2 and RBF2 are required to regulate different sets of target genes in different tissues
Title: Retinoblastoma family 2 is required in vivo for the tissue-specific repression of dE2F2 target genes.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
contributes_to DNA-binding transcription repressor activity, RNA polymerase II-specific	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA endoreduplication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA endoreduplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in G1/S transition of mitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in endomitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in imaginal disc development	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of G1/S transition of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	TAS Traceable Author Statement more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of DRM complex	IDA Inferred from Direct Assay more info	PubMed
part_of Myb complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase II transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Rb-E2F complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: E2F transcription factor 2

Names: CG1071-PA; CG1071-PB; E2f2-PA; E2f2-PB

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

21154293..21156749 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001299186.1 → NP_001286115.1 E2F transcription factor 2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286115.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O77051

UniProtKB/TrEMBL

X2J9B3

Conserved Domains (2) summary

pfam02319
Location:77 → 138

E2F_TDP; E2F/DP family winged-helix DNA-binding domain

cl22411
Location:159 → 218

E2F_DD; Dimerization domain of E2F transcription factors
NM_058007.5 → NP_477355.1 E2F transcription factor 2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477355.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O77051

UniProtKB/TrEMBL

X2J9B3

Related

FBpp0081029

Conserved Domains (2) summary

pfam02319
Location:77 → 138

E2F_TDP; E2F/DP family winged-helix DNA-binding domain

cl22411
Location:159 → 218

E2F_DD; Dimerization domain of E2F transcription factors