RNaseZ Ribonuclease Z [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: RNaseZprovided by FlyBase
Official Full Name: Ribonuclease Zprovided by FlyBase
Primary source: FLYBASE:FBgn0028426
Locus tag: Dmel_CG3298
See related: AllianceGenome:FB:FBgn0028426
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG3298; Dmel\CG3298; dRNase Z; dRNaseZ; dtRNaseZ; jhI-1; JhI-1; JHI-1; RNZ; tRNase Z; tRNAse Z; tRNase Z[L]; tRNAseZ
Summary: Enables 3'-tRNA processing endoribonuclease activity. Involved in several processes, including RNA processing; germ cell proliferation; and positive regulation of translation. Located in cytosol; mitochondrion; and nucleus. Is expressed in several structures, including embryonic imaginal tissue; female germline cell; gonad; larval imaginal tissue; and somatic precursor cell. Used to study cardiomyopathy and combined oxidative phosphorylation deficiency 17. Human ortholog(s) of this gene implicated in combined oxidative phosphorylation deficiency 17. Orthologous to human ELAC2 (elaC ribonuclease Z 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 47A1-47A1; 2-62 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (10307030..10309794, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (6194535..6197299, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cardiac RNase Z edited via CRISPR-Cas9 drives heart hypertrophy in Drosophila.
Title: Cardiac RNase Z edited via CRISPR-Cas9 drives heart hypertrophy in Drosophila.
The linker region was explored by Ala-scanning through two conserved regions of D. melanogaster tRNase Z: NdomTprox, located at the carboxy end of the amino domain proximal to the linker, and Tflex, a flexible site in the linker. Periodic substitutions in a hydrophobic patch (F329 and L332) at the carboxy end of NdomTprox show 2,700 and 670-fold impairment relative to wild type
Title: Substitutions in conserved regions preceding and within the linker affect activity and flexibility of tRNase ZL, the long form of tRNase Z.
The study implicates Drosophila RNase Z(L) in a novel retrograde signaling pathway initiated by the damage in mitochondria and manifested in a cell cycle delay before the mitotic entry.
Title: Knockout of Drosophila RNase ZL impairs mitochondrial transcript processing, respiration and cell cycle progression.
RNase Z(L) activity is essential for all phases of fly development that involve cell division, including growth of adult tissue progenitors during larval and metamorphic stages, and gametogenesis in adults.
Title: Developmental roles of Drosophila tRNA processing endonuclease RNase ZL as revealed with a conditional rescue system.
The study shows that dRNaseZ is necessary for 3'-end processing of nuclear & mitochondrial tRNAs. In mitotically growing imaginal discs it is required for cell proliferation and/or viability. In endoreplicating salivary glands, it controls organ size.
Title: RNAi knockdown of dRNaseZ, the Drosophila homolog of ELAC2, impairs growth of mitotic and endoreplicating tissues.
Residues in two homology blocks on the amino side of the tRNase Z His domain contribute unexpectedly to pre-tRNA 3' end processing
Title: Residues in two homology blocks on the amino side of the tRNase Z His domain contribute unexpectedly to pre-tRNA 3' end processing.
conserved, functionally important residues within the His cluster of tRNase Z are thus involved in catalysis, but substrate recognition and binding functions must reside elsewhere in the protein.
Title: Residues in the conserved His domain of fruit fly tRNase Z that function in catalysis are not involved in substrate recognition or binding.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 3'-tRNA processing endoribonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-tRNA processing endoribonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables 3'-tRNA processing endoribonuclease activity	IEA Inferred from Electronic Annotation more info
enables 3'-tRNA processing endoribonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in germ cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial polycistronic RNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial tRNA 3'-end processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial tRNA 3'-end processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA 3'-end processing	IDA Inferred from Direct Assay more info	PubMed
involved_in tRNA 3'-end processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA processing	IEA Inferred from Electronic Annotation more info
involved_in tRNA splicing, via endonucleolytic cleavage and ligation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral cord development	HMP more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial nucleoid	ISS Inferred from Sequence or Structural Similarity more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	NAS Non-traceable Author Statement more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed

General protein information

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Preferred Names: ribonuclease Z

Names: CG3298-PB; RNaseZ-PB; juvenile hormone-inducible protein 1; tRNA 3'-processing endonuclease; tRNase Z

NP_724916.1

EC 3.1.26.-

EC 3.1.26.11

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

10307030..10309794 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_165763.3 → NP_724916.1 ribonuclease Z [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724916.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8MKW7, Q8MRT4, Q9GZ73

Related

FBpp0087449

Conserved Domains (3) summary

COG1234
Location:456 → 726

ElaC; Ribonuclease BN, tRNA processing enzyme [Translation, ribosomal structure and biogenesis]

cd07718
Location:457 → 655

RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold; Ribonuclease Z ELAC1, C-terminus of ELAC2, and related proteins; MBL-fold metallo-hydrolase domain

cl23716
Location:61 → 262

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain