Buffy buffy [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Buffyprovided by FlyBase
Official Full Name: buffyprovided by FlyBase
Primary source: FLYBASE:FBgn0040491
Locus tag: Dmel_CG8238
See related: AllianceGenome:FB:FBgn0040491
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Bcl-2-48AE; buffy; BUFFY; Buffy/Borg2; Buffy/dBorg-2; Buffy/DBorg-2; CG8238; dBorg-2; dBORG-2/BUFFY; dBorg2; dbuffy; Dbx; Dmel\CG8238; Drob2
Summary: Predicted to enable channel activity. Involved in several processes, including cellular response to starvation; ectopic germ cell programmed cell death; and positive regulation of macroautophagy. Located in endoplasmic reticulum. Part of Bcl-2 family protein complex. Is active in mitochondrion. Is expressed in several structures, including anterior-posterior subdivision of organism; mesoderm; and salivary gland. Used to study Parkinson's disease. Orthologous to human BOK (BCL2 family apoptosis regulator BOK). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 48C1-48C1; 2-65 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (11679492..11688557)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (7566997..7576062)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bcl-2 Orthologues, Buffy and Debcl, Can Suppress Drp1-Dependent Age-Related Phenotypes in Drosophila.
Title: Bcl-2 Orthologues, Buffy and Debcl, Can Suppress Drp1-Dependent Age-Related Phenotypes in Drosophila.
study investigates whether Buffy would rescue the loss of pyridoxal kinase induced phenotypes in Drosophila
Title: A loss of Pdxk model of Parkinson disease in Drosophila can be suppressed by Buffy.
Buffy inhibition results in shortened lifespan and impaired locomotor function and represents a novel model of Parkinson disease in Drosophila melanogaster. The overexpression of Buffy improves healthspan and counteracts the effects of alpha-synuclein expression to demonstrate its protective function.
Title: The Bcl-2 homologue Buffy rescues α-synuclein-induced Parkinson disease-like phenotypes in Drosophila.
Buffy has a role enhancing the loss of parkin and suppressing the loss of Pink1 phenotypes in Drosophila
Title: Overexpression of Buffy enhances the loss of parkin and suppresses the loss of Pink1 phenotypes in Drosophila.
The inhibition of porin phenocopies Parkinson disease phenotypes in Drosophila, while the overexpression of Buffy can counteract these phenotypes to improve the overall "healthspan" of the organism.
Title: Loss of porin function in dopaminergic neurons of Drosophila is suppressed by Buffy.
The buffy null mutant reacted to starvation with the expected responses such as inhibition of target of rapamycin (Tor) signaling, autophagy initiation and mobilization of stored lipids.
Title: Drosophila larvae lacking the bcl-2 gene, buffy, are sensitive to nutrient stress, maintain increased basal target of rapamycin (Tor) signaling and exhibit characteristics of altered basal energy metabolism.
Developmental programmed cell death regulation in the fly does not rely upon the Bcl-2 proteins.
Title: Drosophila Bcl-2 proteins participate in stress-induced apoptosis, but are not required for normal development.
BUFFY and DEBCL localize to distinct intracellular membranes, DEBCL predominantly to mitochondria and BUFFY to endoplasmic reticula
Title: Molecular determinants of the subcellular localization of the Drosophila Bcl-2 homologues DEBCL and BUFFY.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables channel activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cellular response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ectopic germ cell programmed cell death	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in extrinsic apoptotic signaling pathway in absence of ligand	IBA Inferred from Biological aspect of Ancestor more info
involved_in intrinsic apoptotic signaling pathway in response to DNA damage	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of programmed cell death	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of programmed cell death	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in release of cytochrome c from mitochondria	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of Bcl-2 family protein complex	IPI Inferred from Physical Interaction more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrial outer membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: buffy

Names: 48A-E Bcl-2-like; Buffy-PA; CG8238-PA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

11679492..11688557

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_078978.2 → NP_523702.1 buffy [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523702.1

Status: REVIEWED

UniProtKB/TrEMBL

Q8T8Y5, Q9NGX3, Q9V612

Related

FBpp0087182

Conserved Domains (1) summary

cd06845
Location:99 → 250

Bcl-2_like; Apoptosis regulator proteins of the Bcl-2 family, named after B-cell lymphoma 2. This alignment model spans what have been described as Bcl-2 homology regions BH1, BH2, BH3, and BH4. Many members of this family have an additional C-terminal transmembrane ...