Ncf2 neutrophil cytosolic factor 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ncf2provided by RGD
Official Full Name: neutrophil cytosolic factor 2provided by RGD
Primary source: RGD:1309424
See related: EnsemblRapid:ENSRNOG00000028016 AllianceGenome:RGD:1309424
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: NCF-2; p67phox
Summary: Predicted to enable small GTPase binding activity and superoxide-generating NADPH oxidase activator activity. Predicted to contribute to superoxide-generating NAD(P)H oxidase activity. Involved in several processes, including response to hyperoxia; response to laminar fluid shear stress; and response to progesterone. Predicted to be located in acrosomal vesicle; cytosol; and membrane. Predicted to be part of NADPH oxidase complex. Used to study proteinuria; renal fibrosis; and visual epilepsy. Biomarker of hypertension and obesity. Human ortholog(s) of this gene implicated in Alzheimer's disease; autosomal recessive chronic granulomatous disease 2; and rheumatoid arthritis. Orthologous to human NCF2 (neutrophil cytosolic factor 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Spleen (RPKM 78.3), Lung (RPKM 54.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 13q21

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	13	NC_086031.1 (67505492..67536015)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	13	NC_051348.1 (64955622..64986144)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	13	NC_005112.4 (70226441..70259019)

Chromosome 13 - NC_086031.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

increased mTAL luminal flow results in increases in intracellular and mitochondrial H2O2, which are dependent on the presence of Nox4, and that p67phox/Nox2 accounts solely for increases in O2 (.-) production
Title: Role of Nox4 and p67phox subunit of Nox2 in ROS production in response to increased tubular flow in the mTAL of Dahl salt-sensitive rats.
it is concluded that: (i) melatonin beneficial action against diabetic nephropathy involves attenuation of the excessive activity of Nox; and (ii) the mechanism of melatonin inhibitory effect on Nox is based on the mitigation of expression and membrane translocation of its regulatory subunits.
Title: Melatonin nephroprotective action in Zucker diabetic fatty rats involves its inhibitory effect on NADPH oxidase.
Exercise Training attenuates the increased expression of p47(phox) and p67(phox) in the hearts of diabetic rats which could be an underlying mechanism for improving intracardiac matrix and thus cardiac function and prevent cardiac remodeling in diabetic cardiomyopathy.
Title: Exercise Training Attenuates Upregulation of p47(phox) and p67(phox) in Hearts of Diabetic Rats.
p67(phox) subunit of NADPH oxidase 2 plays an important role in the excess production of ROS from mitochondria in the renal medulla of the Dahl salt-sensitive rat.
Title: Effects of p67phox on the mitochondrial oxidative state in the kidney of Dahl salt-sensitive rats: optical fluorescence 3-D cryoimaging.
The levels of cytosolic p67Phox, p47Phox, and p40Phox were increased in membrane fractions following traumatic brain injury.
Title: A time course of NADPH-oxidase up-regulation and endothelial nitric oxide synthase activation in the hippocampus following neurotrauma.
when the p67(phox)subunit of nicotinamide adenine dinucleotide phosphate-oxidase is not functional in the salt-sensitive rats, high salt diet does not cause reduced renal medullary blood flow and salt-sensitive hypertension is attenuated
Title: Null mutation of the nicotinamide adenine dinucleotide phosphate-oxidase subunit p67phox protects the Dahl-S rat from salt-induced reductions in medullary blood flow and glomerular filtration rate.
Data indicate that green tea polyphenols (GTPs) treatments protected against the high glucose (HG)-induced over-expressions of p22phox and p67phox (NCF2).
Title: Tea polyphenols alleviate high fat and high glucose-induced endothelial hyperpermeability by attenuating ROS production via NADPH oxidase pathway.
The expression of NCF2 was upregulated after IL-4 treatment for 4 h, but it was downregulated after 8-24 h. It was suppressesd by amyloid beta or dbcAMP.
Title: Regulation of NADPH oxidase gene expression with PKA and cytokine IL-4 in neurons and microglia.
Candesartan may protect neurons by decreasing extracellular glutamate immediately after reperfusion and by attenuating oxidative stress via a modulation of the AT1-R signaling that occurs during ischemic insult.
Title: Neuroprotective effects of angiotensin II type 1 receptor (AT1-R) blocker via modulating AT1-R signaling and decreased extracellular glutamate levels.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables superoxide-generating NAD(P)H oxidase activity	IEA Inferred from Electronic Annotation more info
contributes_to superoxide-generating NAD(P)H oxidase activity	ISO Inferred from Sequence Orthology more info
enables superoxide-generating NADPH oxidase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables superoxide-generating NADPH oxidase activator activity	IEA Inferred from Electronic Annotation more info
enables superoxide-generating NADPH oxidase activator activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to hormone stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in phagocytosis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of blood pressure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of reactive oxygen species biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in respiratory burst	IEA Inferred from Electronic Annotation more info
involved_in response to activity	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to glucose	IDA Inferred from Direct Assay more info	PubMed
involved_in response to hyperoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to laminar fluid shear stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	IDA Inferred from Direct Assay more info	PubMed
involved_in response to organic cyclic compound	IDA Inferred from Direct Assay more info	PubMed
involved_in response to progesterone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in superoxide anion generation	IBA Inferred from Biological aspect of Ancestor more info
involved_in superoxide anion generation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within superoxide anion generation	ISO Inferred from Sequence Orthology more info
involved_in superoxide anion generation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of NADPH oxidase complex	IEA Inferred from Electronic Annotation more info
part_of NADPH oxidase complex	ISO Inferred from Sequence Orthology more info
located_in acrosomal vesicle	IEA Inferred from Electronic Annotation more info
located_in acrosomal vesicle	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neutrophil cytosol factor 2

Names: 67 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001395185.1 → NP_001382114.1 neutrophil cytosol factor 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000013

UniProtKB/TrEMBL

A6ICU1, R4I3Z7

Related

ENSRNOP00000079682.1, ENSRNOT00000088724.3

Conserved Domains (6) summary

cd06406
Location:351 → 429

PB1_P67; A PB1 domain is present in p67 proteins which forms a signaling complex with p40, a crucial step for activation of NADPH oxidase during phagocytosis. PB1 domain is a modular domain mediating specific protein-protein interactions which play a role in ...

cd11871
Location:244 → 297

SH3_p67phox_N; N-terminal (or first) Src Homology 3 domain of the p67phox subunit of NADPH oxidase

cd12046
Location:461 → 512

SH3_p67phox_C; C-terminal (or second) Src Homology 3 domain of the p67phox subunit of NADPH oxidase

COG0457
Location:4 → 122

TPR; Tetratricopeptide (TPR) repeat [General function prediction only]

sd00006
Location:6 → 32

TPR; TPR repeat [structural motif]

pfam00515
Location:71 → 99

TPR_1; Tetratricopeptide repeat

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086031.1 Reference GRCr8

Range

67505492..67536015

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_001100984.1: Suppressed sequence

Description

NM_001100984.1: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.