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Isha Insulator su(Hw) mRNA adaptor [ Drosophila melanogaster (fruit fly) ]

Gene ID: 37411, updated on 4-Jan-2025

Summary

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Official Symbol
Ishaprovided by FlyBase
Official Full Name
Insulator su(Hw) mRNA adaptorprovided by FlyBase
Primary source
FLYBASE:FBgn0034598
Locus tag
Dmel_CG4266
See related
AllianceGenome:FB:FBgn0034598
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
cg4266; CG4266; Dmel\CG4266
Summary
Enables chromatin binding activity and mRNA binding activity. Involved in negative regulation of transcription by RNA polymerase II. Located in chromatin. Is expressed in larval ventral nerve cord and organism. Orthologous to human SCAF4 (SR-related CTD associated factor 4) and SCAF8 (SR-related CTD associated factor 8). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs
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Genomic context

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See Isha in Genome Data Viewer
Location:
57C6-57C7; 2-93 cM
Exon count:
10
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (21161713..21168659, complement)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (17049218..17056164, complement)

Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene transfer RNA:Glycine-GCC 1-12 Neighboring gene transfer RNA:Tryptophan-CCA 2-4 Neighboring gene hinge1 Neighboring gene Like Sm 1 Neighboring gene Xeroderma pigmentosum D

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Variants in SCAF4 Cause a Neurodevelopmental Disorder and Are Associated with Impaired mRNA Processing. Fliedner A, et al. Am J Hum Genet, 2020 Sep 3. PMID 32730804, Free PMC Article
  2. Isha is a su(Hw) mRNA-binding protein required for gypsy insulator function. Bag I, et al. G3 (Bethesda), 2022 Aug 25. PMID 35708663, Free PMC Article
  3. The Drosophila retinoblastoma binding protein 6 family member has two isoforms and is potentially involved in embryonic patterning. Hull R, et al. Int J Mol Sci, 2015 May 6. PMID 25955646, Free PMC Article
  4. The complex I subunit NDUFA10 selectively rescues Drosophila pink1 mutants through a mechanism independent of mitophagy. Pogson JH, et al. PLoS Genet, 2014 Nov. PMID 25412178, Free PMC Article
  5. Large-scale screen for modifiers of ataxin-3-derived polyglutamine-induced toxicity in Drosophila. VoSSfeldt H, et al. PLoS One, 2012. PMID 23139745, Free PMC Article

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Variants in SCAF4 Cause a Neurodevelopmental Disorder and Are Associated with Impaired mRNA Processing.
    Title: Variants in SCAF4 Cause a Neurodevelopmental Disorder and Are Associated with Impaired mRNA Processing.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables chromatin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables mRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables mRNA binding ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
Process Evidence Code Pubs
involved_in negative regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in chromatin IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
insulator su(Hw) mRNA adaptor
Names
CG4266-PA
CG4266-PB
CG4266-PC
CG4266-PD
Isha-PA
Isha-PB
Isha-PC
Isha-PD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033778.4 Reference assembly

    Range
    21161713..21168659 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001299735.1 → NP_001286664.1  insulator su(Hw) mRNA adaptor, isoform D [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001286664.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0B4LH25
    Conserved Domains (5) summary
    COG0724
    Location:394 → 499
    RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]
    cd03562
    Location:7 → 125
    CID; CID (CTD-Interacting Domain) domain family; CID is present in several RNA-processing factors such as Pcf11 and Nrd1. Pcf11 is a conserved and essential subunit of the yeast cleavage factor IA, which is required for polyadenylation-dependent 3'-RNA ...
    cd12227
    Location:396 → 472
    RRM_SCAF4_SCAF8; RNA recognition motif in SR-related and CTD-associated factor 4 (SCAF4), SR-related and CTD-associated factor 8 (SCAF8) and similar proteins
    pfam04929
    Location:1112 → 1210
    Herpes_DNAp_acc; Herpes DNA replication accessory factor
    pfam10310
    Location:1053 → 1160
    DUF2413; Protein of unknown function (DUF2413)

  2. NM_137713.3 → NP_611557.2  insulator su(Hw) mRNA adaptor, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_611557.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q9W2K4
    Related
    FBpp0071554
    Conserved Domains (5) summary
    COG0724
    Location:397 → 502
    RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]
    cd03562
    Location:7 → 125
    CID; CID (CTD-Interacting Domain) domain family; CID is present in several RNA-processing factors such as Pcf11 and Nrd1. Pcf11 is a conserved and essential subunit of the yeast cleavage factor IA, which is required for polyadenylation-dependent 3'-RNA ...
    cd12227
    Location:399 → 475
    RRM_SCAF4_SCAF8; RNA recognition motif in SR-related and CTD-associated factor 4 (SCAF4), SR-related and CTD-associated factor 8 (SCAF8) and similar proteins
    pfam04929
    Location:1115 → 1213
    Herpes_DNAp_acc; Herpes DNA replication accessory factor
    pfam10310
    Location:1056 → 1163
    DUF2413; Protein of unknown function (DUF2413)

  3. NM_001274190.2 → NP_001261119.1  insulator su(Hw) mRNA adaptor, isoform C [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001261119.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A2VEJ9
    Conserved Domains (1) summary
    cd03562
    Location:7 → 125
    CID; CID (CTD-Interacting Domain) domain family; CID is present in several RNA-processing factors such as Pcf11 and Nrd1. Pcf11 is a conserved and essential subunit of the yeast cleavage factor IA, which is required for polyadenylation-dependent 3'-RNA ...

  4. NM_001103924.2 → NP_001097394.1  insulator su(Hw) mRNA adaptor, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001097394.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A8DYK5
    Conserved Domains (5) summary
    COG0724
    Location:488 → 593
    RRM; RNA recognition motif (RRM) domain [Translation, ribosomal structure and biogenesis]
    cd03562
    Location:7 → 125
    CID; CID (CTD-Interacting Domain) domain family; CID is present in several RNA-processing factors such as Pcf11 and Nrd1. Pcf11 is a conserved and essential subunit of the yeast cleavage factor IA, which is required for polyadenylation-dependent 3'-RNA ...
    cd12227
    Location:490 → 566
    RRM_SCAF4_SCAF8; RNA recognition motif in SR-related and CTD-associated factor 4 (SCAF4), SR-related and CTD-associated factor 8 (SCAF8) and similar proteins
    pfam04929
    Location:1206 → 1304
    Herpes_DNAp_acc; Herpes DNA replication accessory factor
    pfam10310
    Location:1147 → 1254
    DUF2413; Protein of unknown function (DUF2413)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
A0A0B4LH25 A0A0B4LH25
A2VEJ9 A2VEJ9
A8DYK5 A8DYK5
Q95TR3 Q95TR3
Q9W2K4 Q9W2K4

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