Hsp22 Heat shock protein 22 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Hsp22provided by FlyBase
Official Full Name: Heat shock protein 22provided by FlyBase
Primary source: FLYBASE:FBgn0001223
Locus tag: Dmel_CG4460
See related: AllianceGenome:FB:FBgn0001223
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 22; anon-WO0118547.50; anon-WO0140519.138; CG32041; CG4460; dHsp22; dHSP22; Dmel20.8; Dmel\CG4460; DmHsp22; EP(3)3247; hsp22; HSP22; small hsp locus 67B
Summary: Enables identical protein binding activity and unfolded protein binding activity. Involved in several processes, including chaperone-mediated protein folding; determination of adult lifespan; and response to heat. Located in mitochondrial matrix. Is expressed in ectoderm. Human ortholog(s) of this gene implicated in cataract 9 multiple types. Orthologous to human CRYAA (crystallin alpha A). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 67B2-67B2; 3-29 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (9372931..9374964)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (9366031..9368064)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

60 common DmHsp22-binding mitochondrial partners were detected via immunoaffinity conjugation with mass spectroscopy analysis of mitochondria from HeLa cells transfected with DmHsp22 in non-heat shock condition and after heat shock. Among the partners of DmHsp22, several ATP synthase subunits were found.
Title: Identification of proteins interacting with the mitochondrial small heat shock protein Hsp22 of Drosophila melanogaster: Implication in mitochondrial homeostasis.
This study shows that mutations of arginine to glycine in Hsp22 alpha-crystallin domain induce a number of structural changes, some of which differ from those described in mammalian small heat shock proteins.
Title: Oligomeric structure and chaperone-like activity of Drosophila melanogaster mitochondrial small heat shock protein Hsp22 and arginine mutants in the alpha-crystallin domain.
Together the results are consistent with a role of Hsp22 in the UPR(MT) and in mitochondrial proteostasis.
Title: Changes in Drosophila mitochondrial proteins following chaperone-mediated lifespan extension confirm a role of Hsp22 in mitochondrial UPR and reveal a mitochondrial localization for cathepsin D.
hsp22 could play an important role in relation to stress resistance and adaptation.
Title: Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants.
Hsp22 and Hsp23 play important roles in the recovery from chill coma in adult males, and suggest that these contribute to adaptive responses to fluctuating thermal conditions.
Title: Knocking down expression of Hsp22 and Hsp23 by RNA interference affects recovery from chill coma in Drosophila melanogaster.
The up-regulation of genes of the OXPHOS system in Hsp22+ flies suggest that mitochondrial homeostasis is at the center of Hsp22 beneficial effects on lifespan.
Title: Gene expression profiling implicates OXPHOS complexes in lifespan extension of flies over-expressing a small mitochondrial chaperone, Hsp22.
Data show that the DmHsp22 interacts and inactivates wild type tumor suppressor protein p53, which may be one possible way of its functioning in human cells.
Title: Proproliferative functions of Drosophila small mitochondrial heat shock protein 22 in human cells.
Life span alteration after irradiation in Drosophila melanogaster strains with mutations of Hsf and Hsps.
Title: Life span alteration after irradiation in Drosophila melanogaster strains with mutations of Hsf and Hsps.
Expression of hsp22 and hsp70 transgenes is partially predictive of drosophila survival under normal and stress conditions.
Title: Expression of hsp22 and hsp70 transgenes is partially predictive of drosophila survival under normal and stress conditions.
Drosophila that are not expressing this mitochondrial small Hsp22 have a 40% decrease in lifespan.
Title: Decreased lifespan in the absence of expression of the mitochondrial small heat shock protein Hsp22 in Drosophila.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0947 (e-PCR)
- UniSTS:16609

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	ISM Inferred from Sequence Model more info	PubMed

Process	Evidence Code	Pubs
involved_in chaperone-mediated protein folding	IDA Inferred from Direct Assay more info	PubMed
involved_in chaperone-mediated protein folding	ISM Inferred from Sequence Model more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein refolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to heat	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to heat	IDA Inferred from Direct Assay more info	PubMed
involved_in response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: heat shock protein 22

Names: CG4460-PA; CG4460-PB; Hsp22-PA; Hsp22-PB; heat shock protein hsp22; heat-shock protein 22; protein 22

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

9372931..9374964

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001031944.2 → NP_001027115.1 heat shock protein 22, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001027115.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V1Q5, P02515, Q95SZ4, Q9VSX1

Conserved Domains (2) summary

cd06526
Location:61 → 138

metazoan_ACD; Alpha-crystallin domain (ACD) of metazoan alpha-crystallin-type small(s) heat shock proteins (Hsps). sHsps are small stress induced proteins with monomeric masses between 12 -43 kDa, whose common feature is the Alpha-crystallin domain (ACD). sHsps are ...

COG0071
Location:17 → 154

IbpA; Molecular chaperone IbpA, HSP20 family [Posttranslational modification, protein turnover, chaperones]
NM_001031943.3 → NP_001027114.1 heat shock protein 22, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001027114.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V1Q5, P02515, Q95SZ4, Q9VSX1

Conserved Domains (2) summary

cd06526
Location:61 → 138

metazoan_ACD; Alpha-crystallin domain (ACD) of metazoan alpha-crystallin-type small(s) heat shock proteins (Hsps). sHsps are small stress induced proteins with monomeric masses between 12 -43 kDa, whose common feature is the Alpha-crystallin domain (ACD). sHsps are ...

COG0071
Location:17 → 154

IbpA; Molecular chaperone IbpA, HSP20 family [Posttranslational modification, protein turnover, chaperones]