DnaJ-1 DnaJ-like-1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: DnaJ-1provided by FlyBase
Official Full Name: DnaJ-like-1provided by FlyBase
Primary source: FLYBASE:FBgn0263106
Locus tag: Dmel_CG10578
See related: AllianceGenome:FB:FBgn0263106
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0140519.166; anon-WO0172774.135; CG10578; dhdj-1; dhdj1; dhdJ1; dHdj1; dHDJ1; dHDJ1/HSP40; dHSP40; Dmel\CG10578; DmHsp40; dnaj-1; dnaJ-1; Dnaj-1; DNAJ-1; DnaJ1; DNAJ1; DnaJ1 64EF; droj1; DroJ1; DROJ1; EU3500; Hdj1; Hsp-40; hsp40; Hsp40; HSP40; HSP40/HDJ1
Summary: Enables DNA-binding transcription factor binding activity. Involved in positive regulation of crystal cell differentiation; protein stabilization; and response to heat. Predicted to be active in cytosol. Is expressed in several structures, including adult head; anlage in statu nascendi; cuboidal/columnar epithelium; gut section; and head epidermis primordium. Human ortholog(s) of this gene implicated in congenital myopathy 21. Orthologous to several human genes including DNAJB5 (DnaJ heat shock protein family (Hsp40) member B5). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 64E5-64E5; 3-15 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (5750029..5752189, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (5743129..5745289, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

our data indicate that the MLF/DnaJ-1-dependent increase in Lz level allows the repression of Notch expression and signaling to prevent aberrant blood cell development. Thus our findings establish a functional link between MLF and the co-chaperone DnaJ-1 to control RUNX transcription factor activity and Notch signaling during blood cell development in vivo.
Title: Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis.
Additional studies established that the increased toxicity upon mutating UbS2 stems from disrupting the autoprotective role that pathogenic ataxin-3 has against itself, which depends on the co-chaperone, DnaJ-1. Our data reveal a previously unrecognized balance between pathogenic and potentially therapeutic properties of the ataxin-3-Rad23 interaction; they highlight this interaction as critical for the toxicity of the S
Title: Interaction of the polyglutamine protein ataxin-3 with Rad23 regulates toxicity in Drosophila models of Spinocerebellar Ataxia Type 3.
Through DnaJ-1, our data connect ataxin-3 and Rad23 to protective processes involved with protein folding rather than increased turnover of toxic polyQ species.
Title: The deubiquitinase ataxin-3 requires Rad23 and DnaJ-1 for its neuroprotective role in Drosophila melanogaster.
Expression of DnaJ-1 potently suppresses alpha1ACT-dependent degeneration , concomitant with decreased aggregation of the pathogenic protein. Mutating the nuclear importer karyopherin a3 also leads to reduced toxicity from pathogenic CACNA1A
Title: DnaJ-1 and karyopherin α3 suppress degeneration in a new Drosophila model of Spinocerebellar Ataxia Type 6.
HSP40 and HSP110 function together in protein homeostasis control.
Title: Suppression of polyglutamine protein toxicity by co-expression of a heat-shock protein 40 and a heat-shock protein 110.
A high positive correlation was found between the levels of Hsp40 expression and scores of basal and induced thermotolerance, implying a significant contribution of Hsp40 gene in thermoadaptation.
Title: Differential expression of small heat shock protein genes Hsp23 and Hsp40, and heat shock gene Hsr-omega in fruit flies (Drosophila melanogaster) along a microclimatic gradient.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-folding chaperone binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in chaperone cofactor-dependent protein refolding	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of crystal cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein folding	IEA Inferred from Electronic Annotation more info
involved_in protein folding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to heat	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: DnaJ-like-1

Names: CG10578-PA; CG10578-PB; DnaJ-1-PA; DnaJ-1-PB; Hsp40; dnaJ like1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

5750029..5752189 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079212.4 → NP_523936.2 DnaJ-like-1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523936.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q24133, Q9VRP0

UniProtKB/TrEMBL

Q53ZT0, Q86DS7

Related

FBpp0076829

Conserved Domains (3) summary

cd10747
Location:157 → 320

DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40

COG0484
Location:1 → 333

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

pfam00226
Location:4 → 65

DnaJ; DnaJ domain
NM_168128.3 → NP_729086.1 DnaJ-like-1, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_729086.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q24133, Q9VRP0

UniProtKB/TrEMBL

Q53ZT0, Q86DS7

Related

FBpp0076830

Conserved Domains (3) summary

cd10747
Location:157 → 320

DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40

COG0484
Location:1 → 333

DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]

pfam00226
Location:4 → 65

DnaJ; DnaJ domain