mib1 mind bomb 1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: mib1provided by FlyBase
Official Full Name: mind bomb 1provided by FlyBase
Primary source: FLYBASE:FBgn0263601
Locus tag: Dmel_CG5841
See related: AllianceGenome:FB:FBgn0263601
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG5841; D-mib; D-mib1; Dmel\CG5841; Dmib; dmib1; l(3)72CDa; l(3)72Db; mib; Mib; Mib1; MIB1; mib1/2; mind-bomb
Summary: Enables ubiquitin protein ligase activity. Involved in several processes, including lateral inhibition; positive regulation of Notch signaling pathway; and positive regulation of endocytosis. Located in apical cortex. Human ortholog(s) of this gene implicated in left ventricular noncompaction. Orthologous to human MIB1 (MIB E3 ubiquitin protein ligase 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 72C2-72C2; 3-43 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (16006552..16011812, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (15999652..16004912, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural requirements for activity of Mind bomb1 in Notch signaling.
Title: Structural requirements for activity of Mind bomb1 in Notch signaling.
The authors found that Dl requires ubi for its full function, but can also signal in two ubi-independent modes, one dependent and one independent of Neur. Their analysis suggests that one important role of Dl protein ubiquitination by Mib1 is their release from cis-inhibitory interactions with Notch, enabling them to trans-activate Notch on adjacent cells.
Title: Ubiquitylation-independent activation of Notch signalling by Delta.
While deficient Mib1 signaling leads to overgrowth of the NMJ, ubiquitous overexpression of Mib1 results in a reduction in the number of presynaptic motor neuron boutons and branches
Title: The Notch ligand E3 ligase, Mind Bomb1, regulates glutamate receptor localization in Drosophila.
The ubiquitylation of Delta by both Neuralized and Mindbomb1 was detected, with the two enzymes using distinct docking sites and having different acceptor lysine preferences.
Title: Distinct intracellular motifs of Delta mediate its ubiquitylation and activation by Mindbomb1 and Neuralized.
both Dmib and Epsin are required in these cells for some of the endocytic events that internalize DSL ligands
Title: Distinct roles for Mind bomb, Neuralized and Epsin in mediating DSL endocytosis and signaling in Drosophila.
Mind bomb (D-mib) associates physically and functionally with both Serrate and Delta, and is a positive component of Notch signaling that is required for multiple Neuralized-independent, Notch-dependent developmental processes.
Title: The ubiquitin ligase Drosophila Mind bomb promotes Notch signaling by regulating the localization and activity of Serrate and Delta.
Simultaneous absence of neuralized and mib1 completely abolishes Notch signaling in both lateral inhibition (generating single neural precursors from a field of equipotent cells)and wing dorsoventral boundary formation.
Title: The interplay between DSL proteins and ubiquitin ligases in Notch signaling.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	NAS Non-traceable Author Statement more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISM Inferred from Sequence Model more info	PubMed

Process	Evidence Code	Pubs
involved_in Notch signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lateral inhibition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Notch signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory organ development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: mind bomb 1

Names: CG5841-PA; CG5841-PB; Mib1; Mindbomb1; lethal (3) 72CDa; lethal (3) 72Db; mib1-PA; mib1-PB; mind bomb; mind bomb-1; mind bomb1; mind-bomb; mindbomb; mindbomb 1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

16006552..16011812 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_140569.2 → NP_648826.2 mind bomb 1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_648826.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8MSR3, Q9VUX2

Related

FBpp0075275, FBtr0075520

Conserved Domains (7) summary

cd00204
Location:628 → 756

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd02339
Location:177 → 221

ZZ_Mind_bomb; Zinc finger, ZZ type. Zinc finger present in Drosophila Mind bomb (D-mib) and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Mind bomb is an E3 ubiqitin ligase that has ...

pfam06701
Location:248 → 312

MIB_HERC2; Mib_herc2

pfam12796
Location:704 → 800

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:568 → 620

Ank_4; Ankyrin repeats (many copies)

pfam13920
Location:968 → 1011

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)

sd00045
Location:735 → 767

ANK; ANK repeat [structural motif]
NM_001274984.1 → NP_001261913.1 mind bomb 1, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261913.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PI73

Conserved Domains (7) summary

cd00204
Location:517 → 645

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd02339
Location:66 → 110

ZZ_Mind_bomb; Zinc finger, ZZ type. Zinc finger present in Drosophila Mind bomb (D-mib) and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding. Mind bomb is an E3 ubiqitin ligase that has ...

pfam06701
Location:137 → 201

MIB_HERC2; Mib_herc2

pfam12796
Location:593 → 689

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:457 → 509

Ank_4; Ankyrin repeats (many copies)

pfam13920
Location:857 → 900

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)

sd00045
Location:624 → 656

ANK; ANK repeat [structural motif]