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CYP4F3 cytochrome P450 family 4 subfamily F member 3 [ Homo sapiens (human) ]

Gene ID: 4051, updated on 27-Nov-2024

Summary

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Official Symbol
CYP4F3provided by HGNC
Official Full Name
cytochrome P450 family 4 subfamily F member 3provided by HGNC
Primary source
HGNC:HGNC:2646
See related
Ensembl:ENSG00000186529 MIM:601270; AllianceGenome:HGNC:2646
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CPF3; CYP4F; LTB4H; CYPIVF3
Summary
This gene, CYP4F3, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F8, is approximately 18 kb away. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2019]
Expression
Biased expression in liver (RPKM 51.6), bone marrow (RPKM 29.9) and 6 other tissues See more
Orthologs
all
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Genomic context

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See CYP4F3 in Genome Data Viewer
Location:
19p13.12
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (15640897..15662825)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (15766167..15788209)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (15751707..15773635)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene ribosomal protein L23a pseudogene 2 Neighboring gene cytochrome P450 family 4 subfamily F member 8 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10286 Neighboring gene cytochrome P450 family 4 subfamily F member 10, pseudogene Neighboring gene cytochrome P450 family 4 subfamily F member 12

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. A novel form of cytochrome P-450 family 4 in human polymorphonuclear leukocytes. cDNA cloning and expression of leukotriene B4 omega-hydroxylase. Kikuta Y, et al. J Biol Chem, 1993 May 5. PMID 8486631
  2. Human leukotriene B4 omega-hydroxylase (CYP4F3) gene: molecular cloning and chromosomal localization. Kikuta Y, et al. DNA Cell Biol, 1998 Mar. PMID 9539102
  3. Genetic Polymorphisms in Fatty Acid Metabolism Modify the Association Between Dietary n3: n6 Intake and Risk of Ulcerative Colitis: A Prospective Cohort Study. Ananthakrishnan AN, et al. Inflamm Bowel Dis, 2017 Nov. PMID 28991856, Free PMC Article
  4. Pathway-analysis of published genome-wide association studies of lung cancer: A potential role for the CYP4F3 locus. Yin J, et al. Mol Carcinog, 2017 Jun. PMID 28150878, Free PMC Article
  5. Human cytochrome P450 4F3: structure, functions, and prospects. Corcos L, et al. Drug Metabol Drug Interact, 2012 Apr 19. PMID 22706230

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. High n3:n6 polyunsaturated fatty acids (PUFA) intake was associated with a reduced risk of ulcerative colitis (UC) in individuals with the GG/AG genotype at a single nucleotide polymorphism in CYP4F3 but not those with the AA genotype. The association between dietary n3:n6 PUFA intake and risk of UC may be modified variants at CYP4F3.
    Title: Genetic Polymorphisms in Fatty Acid Metabolism Modify the Association Between Dietary n3: n6 Intake and Risk of Ulcerative Colitis: A Prospective Cohort Study.
  2. The results suggest that a potentially functional single-nucleotide polymorphisms in CYP4F3 (rs4646904) may contribute to the etiology of lung cancer, especially in smokers.
    Title: Pathway-analysis of published genome-wide association studies of lung cancer: A potential role for the CYP4F3 locus.
  3. Children who consumed a higher dietary ratio of omega6/omega3 were susceptible for Crohn's disease if they were also carriers of specific variants of CYP4F3 and FADS2 genes.
    Title: Interactions between the dietary polyunsaturated fatty acid ratio and genetic factors determine susceptibility to pediatric Crohn's disease.
  4. In this short review, emphasis will be placed on the regulation and the functional roles of human CYP4F3--{REVIEW}
    Title: Human cytochrome P450 4F3: structure, functions, and prospects.
  5. can directly stimulate osteoclast differentiation dependent of RANKL
    Title: LTB4 can stimulate human osteoclast differentiation dependent of RANKL.
  6. CYP4F3B is the key enzyme to produce 20-HETE by omega-hydroxylation of arachidonic acid in liver cells
    Title: CYP4F3B is induced by PGA1 in human liver cells: a regulation of the 20-HETE synthesis.
  7. Observational study of gene-disease association. (HuGE Navigator)
    Title: New genetic associations detected in a host response study to hepatitis B vaccine.
  8. By regulating the balance of LTB4 in the lung, LTB4OH may function as a suppressor of lung carcinogenesis.
    Title: Differential modulation of leukotriene B4 synthesis and degradation in human bronchoalveolar lavage cells by lipopolysaccharide and tobacco smoke.
  9. Main fatty acid pathway is the omega-hydroxylation of polyunsaturated fatty acids.
    Title: Cytochromes P450 from family 4 are the main omega hydroxylating enzymes in humans: CYP4F3B is the prominent player in PUFA metabolism.
  10. 3-hydroxystearate and 3-hydroxypalmitate are converted to omega-hydroxylated 3-OHDCA precursors in liver; CYP4F11 and, to a lesser extent, CYP4F2 catalyzed omega-hydroxylation of 3-hydroxystearate; CYP4F3b, CYP4F12, and CYP4A11 had negligible activity.
    Title: Omega oxidation of 3-hydroxy fatty acids by the human CYP4F gene subfamily enzyme CYP4F11.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables 20-aldehyde-leukotriene B4 20-monooxygenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 20-aldehyde-leukotriene B4 20-monooxygenase activity TAS
Traceable Author Statement
more info
  
enables 20-hydroxy-leukotriene B4 omega oxidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 20-hydroxy-leukotriene B4 omega oxidase activity TAS
Traceable Author Statement
more info
  
enables arachidonate omega-hydroxylase activity IEA
Inferred from Electronic Annotation
more info
  
enables heme binding IEA
Inferred from Electronic Annotation
more info
  
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables leukotriene-B4 20-monooxygenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables leukotriene-B4 20-monooxygenase activity TAS
Traceable Author Statement
more info
  
enables long-chain fatty acid omega-1 hydroxylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables long-chain fatty acid omega-hydroxylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables monooxygenase activity TAS
Traceable Author Statement
more info
  
enables very long-chain fatty acid omega-hydroxylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in arachidonate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in arachidonate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in fatty acid omega-oxidation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in icosanoid metabolic process TAS
Traceable Author Statement
more info
  
involved_in leukotriene B4 catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in leukotriene B4 catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in leukotriene metabolic process TAS
Traceable Author Statement
more info
  
involved_in lipoxin A4 metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in lipoxin B4 metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in menaquinone catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in omega-hydroxylase P450 pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in phylloquinone catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in endoplasmic reticulum membrane TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
cytochrome P450 4F3
Names
20-HETE synthase
20-hydroxyeicosatetraenoic acid synthase
cytochrome P-450
cytochrome P450, family 4, subfamily F, polypeptide 3
cytochrome P450, subfamily IVF, polypeptide 3 (leukotriene B4 omega hydroxylase)
cytochrome P450-LTB-omega
docosahexaenoic acid omega-hydroxylase CYP4F3
leukotriene B4 omega hydroxylase
leukotriene-B(4) 20-monooxygenase 2
leukotriene-B(4) omega-hydroxylase 2
leukotriene-B4 20-monooxygenase
NP_000887.2
NP_001186137.1
NP_001186138.1
NP_001356625.1
XP_011526316.1
XP_016882304.1
XP_047294796.1
XP_054177005.1
XP_054177006.1
XP_054177007.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_007964.2 RefSeqGene

    Range
    5002..26930
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_000896.3 → NP_000887.2  cytochrome P450 4F3 isoform a

    See identical proteins and their annotated locations for NP_000887.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes isoform a, which is the same length but has internal differences compared to isoform b.
    Source sequence(s)
    AB002454, AD000685, D12621
    Consensus CDS
    CCDS12332.1
    UniProtKB/Swiss-Prot
    B7Z8Z3, O60634, Q08477, Q5U740
    UniProtKB/TrEMBL
    A0A0A0MQR0
    Related
    ENSP00000221307.6, ENST00000221307.13
    Conserved Domains (1) summary
    pfam00067
    Location:52 → 515
    p450; Cytochrome P450

  2. NM_001199208.2 → NP_001186137.1  cytochrome P450 4F3 isoform b

    See identical proteins and their annotated locations for NP_001186137.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon and contains another alternate in-frame exon compared to variant 1. The resulting isoform (b) is the same length but has a different internal segment compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).
    Source sequence(s)
    AB002454, AD000685, BC136299, DB184202
    Consensus CDS
    CCDS59362.1
    UniProtKB/TrEMBL
    B7Z442
    Related
    ENSP00000466988.1, ENST00000591058.5
    Conserved Domains (1) summary
    pfam00067
    Location:52 → 515
    p450; Cytochrome P450

  3. NM_001199209.2 → NP_001186138.1  cytochrome P450 4F3 isoform b

    See identical proteins and their annotated locations for NP_001186138.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, lacks an alternate in-frame exon, and contains another alternate in-frame exon compared to variant 1. The resulting isoform (b) is the same length but has a different internal segment compared to isoform a. Variants 2, 3, and 4 all encode the same isoform (b).
    Source sequence(s)
    AD000685, AK316136, BC136299, DB184202
    Consensus CDS
    CCDS59362.1
    UniProtKB/TrEMBL
    B7Z442
    Related
    ENSP00000466395.1, ENST00000586182.6
    Conserved Domains (1) summary
    pfam00067
    Location:52 → 515
    p450; Cytochrome P450

  4. NM_001369696.1 → NP_001356625.1  cytochrome P450 4F3 isoform b

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4), as well as variants 2 and 3, encodes isoform b.
    Source sequence(s)
    AD000685
    Consensus CDS
    CCDS59362.1
    UniProtKB/TrEMBL
    B7Z442
    Related
    ENSP00000468105.1, ENST00000585846.1
    Conserved Domains (1) summary
    pfam00067
    Location:52 → 515
    p450; Cytochrome P450

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

    Range
    15640897..15662825
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047438840.1 → XP_047294796.1  cytochrome P450 4F3 isoform X2

  2. XM_017026815.2 → XP_016882304.1  cytochrome P450 4F3 isoform X1

    Conserved Domains (1) summary
    pfam00067
    Location:52 → 378
    p450; Cytochrome P450

  3. XM_011528014.4 → XP_011526316.1  cytochrome P450 4F3 isoform X2

    See identical proteins and their annotated locations for XP_011526316.1

    UniProtKB/TrEMBL
    B7Z449
    Conserved Domains (1) summary
    pfam00067
    Location:1 → 366
    p450; Cytochrome P450

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060943.1 Alternate T2T-CHM13v2.0

    Range
    15766167..15788209
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054321031.1 → XP_054177006.1  cytochrome P450 4F3 isoform X2

  2. XM_054321030.1 → XP_054177005.1  cytochrome P450 4F3 isoform X1

  3. XM_054321032.1 → XP_054177007.1  cytochrome P450 4F3 isoform X2

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q08477.2 GenPept UniProtKB/Swiss-Prot:Q08477

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