Hph HIF prolyl hydroxylase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Hphprovided by FlyBase
Official Full Name: HIF prolyl hydroxylaseprovided by FlyBase
Primary source: FLYBASE:FBgn0264785
Locus tag: Dmel_CG44015
See related: AllianceGenome:FB:FBgn0264785
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 0303/04; 31543; anon-EST:Posey69; CG1114; CG14665; CG2676; CG31543; CG44015; CR31541; dHPH; Dm.egl-9; Dmel\CG44015; dmHPH; dPHD; dPHD2; fatiga; Fatiga; fga; Fga; FgaB; hph; l(3)02255; l(3)82Fe; l(3)S030304; PHD
Old locus tag: Dmel_CG31543; Dmel_CR31541
Summary: Enables peptidyl-proline dioxygenase activity. Involved in cellular response to hypoxia; positive regulation of cell growth; and protein export from nucleus. Located in nucleoplasm. Is expressed in several structures, including anterior endoderm; anterior endoderm anlage; embryonic/larval midgut primordium; posterior endoderm; and ventral ectoderm. Human ortholog(s) of this gene implicated in familial erythrocytosis 3; polycythemia; and renal cell carcinoma. Orthologous to several human genes including EGLN2 (egl-9 family hypoxia inducible factor 2). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 82F7-82F8; 3-47.5 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (5257037..5269507)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (1082759..1095229)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

miR-190 is a novel regulator of the hypoxia response that represses the oxygen sensor Fatiga, leading to HIFalpha stabilization and enhancement of hypoxic responses.
Title: miR-190 Enhances HIF-Dependent Responses to Hypoxia in Drosophila by Inhibiting the Prolyl-4-hydroxylase Fatiga.
fgaB but not fgaA is induced in hypoxia, in a Sima-dependent manner, through a HIF-Responsive Element localized in the first intron of fgaA
Title: Oxygen sensing in Drosophila: multiple isoforms of the prolyl hydroxylase fatiga have different capacity to regulate HIFalpha/Sima.
Data show that extra tracheal sprouting during hypoxia depends on the Hypoxia-Inducible Factor (HIF)-alpha homolog Sima and on the HIF-prolyl hydroxylase Fatiga that operates as an oxygen sensor.
Title: Cell autonomy of HIF effects in Drosophila: tracheal cells sense hypoxia and induce terminal branch sprouting.
Our data suggest that Hph, in addition to its function in hypoxic response, is a regulator of cellular growth and that it is a key mediator for CycD/Cdk4.
Title: Drosophila cyclin D/Cdk4 requires Hif-1 prolyl hydroxylase to drive cell growth.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables L-ascorbic acid binding	IEA Inferred from Electronic Annotation more info
enables ferrous iron binding	IBA Inferred from Biological aspect of Ancestor more info
enables hypoxia-inducible factor-proline dioxygenase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	ISM Inferred from Sequence Model more info	PubMed
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info
enables peptidyl-proline 4-dioxygenase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables peptidyl-proline dioxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables peptidyl-proline dioxygenase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to hypoxia	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in terminal branching, open tracheal system	HMP more info	PubMed

Component	Evidence Code	Pubs
located_in nucleoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: HIF prolyl hydroxylase

Names: CG44015-PA; CG44015-PB; CG44015-PC; CG44015-PD; HIF prolyl 4-hydroxylase; HIF-1a prolyl hyroxylase; Hph-PA; Hph-PB; Hph-PC; Hph-PD; fatiga; lethal (3) 82Fe; lethal (3) S030304; lopped; prolyl-4-hydroxylase

NP_001287171.1

EC 1.14.-.-

EC 1.14.11.29

NP_649525.1

EC 1.14.-.-

EC 1.14.11.29

NP_730906.1

EC 1.14.-.-

EC 1.14.11.29

NP_730908.1

EC 1.14.-.-

EC 1.14.11.29

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

5257037..5269507

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001300242.1 → NP_001287171.1 HIF prolyl hydroxylase, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001287171.1

Status: REVIEWED

UniProtKB/TrEMBL

Q8SX21, Q9VN96

Conserved Domains (2) summary

smart00702
Location:220 → 400

P4Hc; Prolyl 4-hydroxylase alpha subunit homologues

pfam01753
Location:30 → 67

zf-MYND; MYND finger
NM_169036.2 → NP_730906.1 HIF prolyl hydroxylase, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730906.1

Status: REVIEWED

UniProtKB/TrEMBL

Q8SX21, Q9VN96

Related

FBpp0306391

Conserved Domains (2) summary

smart00702
Location:220 → 400

P4Hc; Prolyl 4-hydroxylase alpha subunit homologues

pfam01753
Location:30 → 67

zf-MYND; MYND finger
NM_169037.3 → NP_730908.1 HIF prolyl hydroxylase, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730908.1

Status: REVIEWED

UniProtKB/TrEMBL

Q8I0C2

Related

FBpp0306389

Conserved Domains (1) summary

smart00702
Location:20 → 200

P4Hc; Prolyl 4-hydroxylase alpha subunit homologues
NM_141268.3 → NP_649525.1 HIF prolyl hydroxylase, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_649525.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VN98

Related

FBpp0306390

Conserved Domains (1) summary

smart00702
Location:67 → 247

P4Hc; Prolyl 4-hydroxylase alpha subunit homologues