Sas-4 Spindle assembly abnormal 4 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Sas-4provided by FlyBase
Official Full Name: Spindle assembly abnormal 4provided by FlyBase
Primary source: FLYBASE:FBgn0011020
Locus tag: Dmel_CG10061
See related: AllianceGenome:FB:FBgn0011020
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CENPJ; CG10061; CPAP; d-SAS-4; D-sas4; D-Sas4; dCPAP; Dmel\CG10061; DmSas4; dsas-4; Dsas-4; DSas-4; DSAS-4; Dsas4; DSas4; l(3)s2214; l(3)S2214; sas-4; SAS-4; sas4; Sas4; SAS4
Summary: Enables molecular adaptor activity. Involved in several processes, including microtubule cytoskeleton organization; protein localization to pericentriolar material; and syncytial blastoderm mitotic cell cycle. Located in centriole and pericentriolar material. Used to study microcephaly and primary autosomal recessive microcephaly 6. Human ortholog(s) of this gene implicated in Seckel syndrome 4; primary autosomal recessive microcephaly; and primary autosomal recessive microcephaly 6. Orthologous to human CENPJ (centromere protein J). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 84C6-84C7; 3-48 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (7151367..7154219)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (2977089..2979941)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Singularity of the Drosophila Male Germ Cell Centriole: The Asymmetric Distribution of Sas4 and Sas6.
Title: The Singularity of the Drosophila Male Germ Cell Centriole: The Asymmetric Distribution of Sas4 and Sas6.
Here, the authors show that PLK4 phosphorylates its centriole substrate STIL on a conserved site, S428, to promote STIL binding to CPAP. This phospho-dependent binding interaction is conserved in Drosophila and facilitates the stable incorporation of both STIL and CPAP into the centriole.
Title: PLK4 promotes centriole duplication by phosphorylating STIL to link the procentriole cartwheel to the microtubule wall.
These observations identify Plk1/Polo kinase regulation of Sas-4 as essential for efficient pericentriolar material expansion.
Title: Plk1/Polo Phosphorylates Sas-4 at the Onset of Mitosis for an Efficient Recruitment of Pericentriolar Material to Centrosomes.
By halfway through pupal life, the centriole number decreases and structural defects, ranging from being incomplete or lacking B-tubules, are detected. Asl, Ana1 and Sas-4 are still present, suggesting that the centriole integrity does not depend on these proteins.
Title: The developing Drosophila eye - a new model to study centriole reduction.
Cdk1 phosphorylates the conserved centriole protein Sas-4 during mitosis. This creates a Polo-docking site that helps recruit Polo to daughter centrioles.
Title: Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes.
The authors show here, however, that Cnn, Spd-2 and Asl are not recruited to the mother centriole as part of a complex with Sas-4.
Title: Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP.
Inactivation of the spindle assembly checkpoint in the sas-4 mutant impairs neuroblast proliferation and disrupts euploidy.
Title: Spindle assembly checkpoint inactivation fails to suppress neuroblast tumour formation in aurA mutant Drosophila.
Sas-4 acts as a vehicle to tether PCM complexes to centrioles independent of its well-known role in centriole duplication
Title: Conserved TCP domain of Sas-4/CPAP is essential for pericentriolar material tethering during centrosome biogenesis.
Data indicate that interphase centrioles are closely associated with Sas-4, Spd-2, Polo kinase, Pericentrin-like protein (Dplp), Asterless (Asl), Plk4 kinase, Centrosomin (Cnn) and gamma-tubulin.
Title: Structured illumination of the interface between centriole and peri-centriolar material.
Mutations in this conserved sequence also eliminate d-SAS-4's microtubule-destabilizing activity, suggesting that d-SAS-4 and CPAP may play similar roles within cells.
Title: Functional characterization of the microtubule-binding and -destabilizing domains of CPAP and d-SAS-4.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables molecular adaptor activity	IPI Inferred from Physical Interaction more info	PubMed
enables tubulin binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in asymmetric cell division	IDA Inferred from Direct Assay more info	PubMed
involved_in asymmetric cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centriole elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in centriole replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome separation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cilium assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in locomotion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to pericentriolar material	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in syncytial blastoderm mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with centriole	IDA Inferred from Direct Assay more info	PubMed
located_in centriole	IDA Inferred from Direct Assay more info	PubMed
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	HDA more info	PubMed
located_in pericentriolar material	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: spindle assembly abnormal 4

Names: CG10061-PA; DSas-4; Sas-4-PA; drosophila Sas-4; spindle assembly abnormal 4 ortholog; spindle assembly abnormal 4 orthologue

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

7151367..7154219

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_141444.2 → NP_649701.2 spindle assembly abnormal 4 [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_649701.2

Status: REVIEWED

UniProtKB/TrEMBL

Q9VI72

Related

FBpp0081088

Conserved Domains (2) summary

pfam07202
Location:717 → 895

Tcp10_C; T-complex protein 10 C-terminus

cl24005
Location:517 → 587

DUF2570; Protein of unknown function (DUF2570)