Ada2b transcriptional Adaptor 2b [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Ada2bprovided by FlyBase
Official Full Name: transcriptional Adaptor 2bprovided by FlyBase
Primary source: FLYBASE:FBgn0037555
Locus tag: Dmel_CG9638
See related: AllianceGenome:FB:FBgn0037555
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: ada2b; Ada2B; ADA2b; Ada2S; BcDNA:LD24527; CG9638; dAda2b; dAda2B; dADA2b; dAda2S; Dmel\CG9638
Summary: Predicted to enable chromatin binding activity and transcription coactivator activity. Involved in several processes, including regulation of DNA-templated transcription; regulation of terminal button organization; and wing disc pattern formation. Located in nucleus and polytene chromosome. Part of H3 histone acetyltransferase complex and SAGA complex. Is expressed in embryonic brain; embryonic/larval midgut; larval ventral nerve cord; and organism. Orthologous to human TADA2B (transcriptional adaptor 2B). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 84F5-84F5; 3-48 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (8236632..8240515, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (4062354..4066237, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts.
Title: Quantitative Fluorescence Resonance Energy Transfer Analysis on the Direct Interaction of Activation-2b with Histone H3/Switch-3B Protein in Arabidopsis Mesophyll Protoplasts.
demonstration that the fly ADA complex has histone acetylation activity on histones and nucleosome substrates; ChIP-Sequencing experiments identified Ada2b peaks that overlap with another SAGA subunit, Spt3, as well as Ada2b peaks that do not overlap with Spt3 suggesting that the ADA complex binds chromosomal sites independent of the larger SAGA complex
Title: Characterization of a metazoan ADA acetyltransferase complex.
different isoforms of dADA2b contribute to the functional variations of dSAGA multiprotein HAT complexes
Title: Functional characterization and gene expression profiling of Drosophila melanogaster short dADA2b isoform-containing dSAGA complexes.
ADA2 C-terminal regions play an important role in the specific incorporation of ADA2 into SAGA- or ATAC-type complexes, which in turn determines H3- or H4-specific histone targeting.
Title: The C-terminal domains of ADA2 proteins determine selective incorporation into GCN5-containing complexes that target histone H3 or H4 for acetylation.
Ada2b is required for viability in Drosophila, and its deletion causes a reduction in histone H3 acetylation.
Title: Drosophila Ada2b is required for viability and normal histone H3 acetylation.
The ADA2b gene, functionally distinct homolog of ADA2a, is essential for the Drosophila development.
Title: The homologous Drosophila transcriptional adaptors ADA2a and ADA2b are both required for normal development but have different functions.
in vivo the two dADA2 proteins showed different localizations on polytene X chromosomes. These results, taken together, suggest that the two Drosophila ADA2 homologues are present in distinct GCN5-containing HAT complexes
Title: Two different Drosophila ADA2 homologues are present in distinct GCN5 histone acetyltransferase-containing complexes.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coactivator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coactivator activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of terminal button organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in wing disc pattern formation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of H3 histone acetyltransferase complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA complex	IDA Inferred from Direct Assay more info	PubMed
part_of SAGA complex	IPI Inferred from Physical Interaction more info	PubMed
part_of SAGA-type complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in polytene chromosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: transcriptional adaptor 2b

Names: Ada2b-PA; Ada2b-PB; Ada2b-PC; CG9638-PA; CG9638-PB; CG9638-PC; transcriptional adapter 2S

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

8236632..8240515 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001275438.1 → NP_001262367.1 transcriptional adaptor 2b, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262367.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KFU2

Conserved Domains (3) summary

COG5114
Location:10 → 342

COG5114; Histone acetyltransferase complex SAGA/ADA, subunit ADA2 [Chromatin structure and dynamics]

cd00167
Location:73 → 115

SANT; 'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains. Tandem copies of the domain bind telomeric DNA tandem repeatsas part of the capping complex. Binding is sequence dependent for repeats which contain the G/C rich motif [C2-3 A (CA)1-6]. The domain is ...

cd02335
Location:11 → 58

ZZ_ADA2; Zinc finger, ZZ type. Zinc finger present in ADA2, a putative transcriptional adaptor, and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding.
NM_001031980.2 → NP_001027151.1 transcriptional adaptor 2b, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001027151.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8I8V0, Q9VHV0

Conserved Domains (3) summary

COG5114
Location:10 → 341

COG5114; Histone acetyltransferase complex SAGA/ADA, subunit ADA2 [Chromatin structure and dynamics]

cd00167
Location:73 → 115

SANT; 'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains. Tandem copies of the domain bind telomeric DNA tandem repeatsas part of the capping complex. Binding is sequence dependent for repeats which contain the G/C rich motif [C2-3 A (CA)1-6]. The domain is ...

cd02335
Location:11 → 58

ZZ_ADA2; Zinc finger, ZZ type. Zinc finger present in ADA2, a putative transcriptional adaptor, and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding.
NM_141516.3 → NP_649773.1 transcriptional adaptor 2b, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_649773.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KFU2

Related

FBpp0081303

Conserved Domains (3) summary

COG5114
Location:10 → 342

COG5114; Histone acetyltransferase complex SAGA/ADA, subunit ADA2 [Chromatin structure and dynamics]

cd00167
Location:73 → 115

SANT; 'SWI3, ADA2, N-CoR and TFIIIB' DNA-binding domains. Tandem copies of the domain bind telomeric DNA tandem repeatsas part of the capping complex. Binding is sequence dependent for repeats which contain the G/C rich motif [C2-3 A (CA)1-6]. The domain is ...

cd02335
Location:11 → 58

ZZ_ADA2; Zinc finger, ZZ type. Zinc finger present in ADA2, a putative transcriptional adaptor, and related proteins. The ZZ motif coordinates two zinc ions and most likely participates in ligand binding or molecular scaffolding.