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Usp8 Ubiquitin specific protease 8 [ Drosophila melanogaster (fruit fly) ]

Gene ID: 42509, updated on 4-Jan-2025

Summary

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Official Symbol
Usp8provided by FlyBase
Official Full Name
Ubiquitin specific protease 8provided by FlyBase
Primary source
FLYBASE:FBgn0038862
Locus tag
Dmel_CG5798
See related
AllianceGenome:FB:FBgn0038862
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG5798; CT18196; Dmel\CG5798; dUBPY; Q9VDD8; ubpy; Ubpy; UbpY; UBPY; UBPY/USP8; usp8; USP8
Summary
Enables cysteine-type deubiquitinase activity. Involved in several processes, including negative regulation of lysosomal protein catabolic process; neuron remodeling; and positive regulation of signal transduction. Located in cytosol. Is expressed in adult head and eye. Human ortholog(s) of this gene implicated in ACTH-secreting pituitary adenoma. Orthologous to human USP8 (ubiquitin specific peptidase 8). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs
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Genomic context

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See Usp8 in Genome Data Viewer
Location:
93C1-93C1; 3-71 cM
Exon count:
9
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (21134262..21138214)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (16959984..16963936)

Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene tRNA methyltransferase 7-34 Neighboring gene Interacts with the C terminus of ELL 2 Neighboring gene SNF4/AMP-activated protein kinase gamma subunit Neighboring gene medial glomeruli Neighboring gene Common Dpr-interacting protein

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. CHMP1B is a target of USP8/UBPY regulated by ubiquitin during endocytosis. Crespo-Yàñez X, et al. PLoS Genet, 2018 Jun. PMID 29933386, Free PMC Article
  2. Inhibition of the deubiquitinase USP8 corrects a Drosophila PINK1 model of mitochondria dysfunction. von Stockum S, et al. Life Sci Alliance, 2019 Apr. PMID 30988163, Free PMC Article
  3. The Deubiquitinating Enzyme UBPY Is Required for Lysosomal Biogenesis and Productive Autophagy in Drosophila. Jacomin AC, et al. PLoS One, 2015. PMID 26571504, Free PMC Article
  4. USP8 Down-Regulation Promotes Parkin-Independent Mitophagy in the Drosophila Brain and in Human Neurons. Mauri S, et al. Cells, 2023 Apr 13. PMID 37190052, Free PMC Article
  5. Antagonistic control of myofiber size and muscle protein quality control by the ubiquitin ligase UBR4 during aging. Hunt LC, et al. Nat Commun, 2021 Mar 3. PMID 33658508, Free PMC Article

See all (84) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. USP8 Down-Regulation Promotes Parkin-Independent Mitophagy in the Drosophila Brain and in Human Neurons.
    Title: USP8 Down-Regulation Promotes Parkin-Independent Mitophagy in the Drosophila Brain and in Human Neurons.
  2. The deubiquitinase USP8 targets ESCRT-III to promote incomplete cell division.
    Title: The deubiquitinase USP8 targets ESCRT-III to promote incomplete cell division.
  3. Dual functions of Rack1 in regulating Hedgehog pathway.
    Title: Dual functions of Rack1 in regulating Hedgehog pathway.
  4. Ubpy loss of function results in the accumulation of autophagosomes due to a blockade of the autophagy flux.
    Title: The Deubiquitinating Enzyme UBPY Is Required for Lysosomal Biogenesis and Productive Autophagy in Drosophila.
  5. these data support a model in which Ubpy plays a dual role in both cargo deubiquitylation and the ESCRT-0 subunit Hrs stability during development.
    Title: Ubpy controls the stability of the ESCRT-0 subunit Hrs in development.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables cysteine-type deubiquitinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine-type deubiquitinase activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine-type deubiquitinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in Ras protein signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in endosome organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in imaginal disc-derived wing margin morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of lysosomal protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neuron remodeling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of canonical Wnt signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of receptor recycling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of smoothened signaling pathway IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in protein deubiquitination IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
ubiquitin specific protease 8
Names
CG5798-PA
CG5798-PB
UBPY ortholog
Usp8-PA
Usp8-PB
NP_001247227.1
NP_650948.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033777.3 Reference assembly

    Range
    21134262..21138214
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_142691.2 → NP_650948.2  ubiquitin specific protease 8, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_650948.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    D0IQK1, Q9VDD8
    Related
    FBpp0083419
    Conserved Domains (4) summary
    cd02674
    Location:564 → 887
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:562 → 886
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam08969
    Location:2 → 113
    USP8_dimer; USP8 dimerization domain
    cl00125
    Location:148 → 278
    RHOD; Rhodanese Homology Domain (RHOD); an alpha beta fold domain found duplicated in the rhodanese protein. The cysteine containing enzymatically active version of the domain is also found in the Cdc25 class of protein phosphatases and a variety of proteins ...

  2. NM_001260298.1 → NP_001247227.1  ubiquitin specific protease 8, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001247227.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    B9EQY7, H1UUC2
    Conserved Domains (2) summary
    cd02674
    Location:35 → 358
    Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:33 → 357
    UCH; Ubiquitin carboxyl-terminal hydrolase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
H1UUC2 H1UUC2
Q8MQX4 Q8MQX4
Q9VDD8 Q9VDD8

Gene LinkOut

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