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Spag8 sperm associated antigen 8 [ Mus musculus (house mouse) ]

Gene ID: 433700, updated on 27-Nov-2024

Summary

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Official Symbol
Spag8provided by MGI
Official Full Name
sperm associated antigen 8provided by MGI
Primary source
MGI:MGI:3056295
See related
Ensembl:ENSMUSG00000066196 AllianceGenome:MGI:3056295
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
SMP-1; MH-SPAG8
Summary
Predicted to enable microtubule binding activity. Involved in flagellated sperm motility. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in cytoplasm and male germ cell nucleus. Is active in axonemal A tubule inner sheath and sperm flagellum. Orthologous to human SPAG8 (sperm associated antigen 8). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Biased expression in testis adult (RPKM 31.1), ovary adult (RPKM 6.1) and 4 other tissues See more
Orthologs
human all
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Genomic context

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See Spag8 in Genome Data Viewer
Location:
4 A5; 4 23.05 cM
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (43651330..43653594, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (43651330..43653594, complement)

Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_10057 Neighboring gene predicted gene 12472 Neighboring gene predicted gene, 42279 Neighboring gene natriuretic peptide receptor 2 Neighboring gene histidine triad nucleotide binding protein 2 Neighboring gene small EDRK-rich factor 2 pseudogene Neighboring gene family with sequence similarity 221, member B Neighboring gene small EDRK-rich factor 2 pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis. Wu H, et al. FEBS Lett, 2010 Jul 2. PMID 20488182
  2. De novo protein identification in mammalian sperm using in situ cryoelectron tomography and AlphaFold2 docking. Chen Z, et al. Cell, 2023 Nov 9. PMID 37865089, Free PMC Article
  3. In-cell structural insight into the stability of sperm microtubule doublet. Tai L, et al. Cell Discov, 2023 Nov 21. PMID 37989994, Free PMC Article
  4. Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility. Zhou L, et al. Cell, 2023 Jun 22. PMID 37295417
  5. A conditional knockout resource for the genome-wide study of mouse gene function. Skarnes WC, et al. Nature, 2011 Jun 15. PMID 21677750, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Results indicate that SPAG8 acts as a regulator of ACT and plays an important role in CREM-ACT-mediated gene transcription during spermatogenesis.
    Title: Sperm associated antigen 8 (SPAG8), a novel regulator of activator of CREM in testis during spermatogenesis.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (1) 

General gene information

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Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables microtubule binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell differentiation IEA
Inferred from Electronic Annotation
more info
  
involved_in flagellated sperm motility IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within positive regulation of protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in single fertilization IEA
Inferred from Electronic Annotation
more info
  
involved_in spermatogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in acrosomal vesicle ISO
Inferred from Sequence Orthology
more info
  
is_active_in axonemal A tubule inner sheath IDA
Inferred from Direct Assay
more info
 PubMed 
located_in axonemal microtubule ISO
Inferred from Sequence Orthology
more info
  
located_in axonemal microtubule ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in male germ cell nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in microtubule organizing center IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in sperm flagellum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
sperm-associated antigen 8
Names
sperm associated antigen 8-like protein
sperm membrane protein 1
sperm membrane protein BS-84

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001007463.2 → NP_001007464.1  sperm-associated antigen 8 isoform 2

    See identical proteins and their annotated locations for NP_001007464.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) contains a 3' terminal exon that extends past a splice site used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AL732626
    Consensus CDS
    CCDS38743.1
    UniProtKB/Swiss-Prot
    Q3V0Q6
    Related
    ENSMUSP00000103502.3, ENSMUST00000107870.3
    Conserved Domains (1) summary
    PHA03307
    Location:56 → 175
    PHA03307; transcriptional regulator ICP4; Provisional

  2. NM_001290462.2 → NP_001277391.1  sperm-associated antigen 8 isoform 1

    See identical proteins and their annotated locations for NP_001277391.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AK132968, AL732626
    Consensus CDS
    CCDS71376.1
    UniProtKB/Swiss-Prot
    B9EKF1, Q3V0Q6, Q5UAV3
    Related
    ENSMUSP00000081696.5, ENSMUST00000084646.11
    Conserved Domains (1) summary
    PHA03307
    Location:56 → 175
    PHA03307; transcriptional regulator ICP4; Provisional

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000070.7 Reference GRCm39 C57BL/6J

    Range
    43651330..43653594 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q3V0Q6.1 GenPept UniProtKB/Swiss-Prot:Q3V0Q6

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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