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spag spaghetti [ Drosophila melanogaster (fruit fly) ]

Gene ID: 43958, updated on 9-Dec-2024

Summary

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Official Symbol
spagprovided by FlyBase
Official Full Name
spaghettiprovided by FlyBase
Primary source
FLYBASE:FBgn0015544
Locus tag
Dmel_CG13570
See related
AllianceGenome:FB:FBgn0015544
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG13570; Dmel\CG13570; l(2)k12101; Rpap3; Spag; SPAG; spagh
Summary
Predicted to enable Hsp70 protein binding activity and Hsp90 protein binding activity. Involved in negative regulation of motor neuron apoptotic process and regulation of locomotor rhythm. Predicted to be located in cytoplasm. Is expressed in adult head. Orthologous to human RPAP3 (RNA polymerase II associated protein 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs
all
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Genomic context

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See spag in Genome Data Viewer
Location:
60C1-60C1; 2-106 cM
Exon count:
6
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (24159384..24161503)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (20046907..20049026)

Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene long non-coding RNA:CR44826 Neighboring gene speck Neighboring gene DnaJ-like-60 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene mitochondrial ribosomal protein S17

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Drosophila DBT Autophosphorylation of Its C-Terminal Domain Antagonized by SPAG and Involved in UV-Induced Apoptosis. Fan JY, et al. Mol Cell Biol, 2015 Jul. PMID 25939385, Free PMC Article
  2. The DnaJ60 gene of Drosophila melanogaster encodes a new member of the DnaJ family of proteins. Iliopoulos I, et al. Biol Chem, 1997 Oct. PMID 9372188
  3. Spaghetti, a homolog of human RPAP3 (RNA polymerase II-associated protein 3), determines the fate of germline stem cells in Drosophila ovary. Chen D, et al. Cell Biol Int, 2018 Jul. PMID 29110400
  4. Drosophila spaghetti and doubletime link the circadian clock and light to caspases, apoptosis and tauopathy. Means JC, et al. PLoS Genet, 2015 May. PMID 25951229, Free PMC Article
  5. A Drosophila immune response against Ras-induced overgrowth. Hauling T, et al. Biol Open, 2014 Apr 15. PMID 24659248, Free PMC Article

See all (54) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Heterozygous mutations in hsp90 (hsp90/+) dramatically accelerated the GSC loss in spag RNAi ovaries
    Title: Spaghetti, a homolog of human RPAP3 (RNA polymerase II-associated protein 3), determines the fate of germline stem cells in Drosophila ovary.
  2. we show that spag knock-down or reduced Dbt activity in circadian cells leads to longer circadian periods and expression of activated initiator caspase only during the middle of the day.
    Title: Drosophila spaghetti and doubletime link the circadian clock and light to caspases, apoptosis and tauopathy.
  3. Data show that the HSP-90 cochaperone spaghetti protein (SPAG) antagonizes casein kinase I epsilon DBT autophosphorylation in S2 cells
    Title: Drosophila DBT Autophosphorylation of Its C-Terminal Domain Antagonized by SPAG and Involved in UV-Induced Apoptosis.
  4. Interaction of Spag with both Hsp70 and Hsp90 suggests a model whereby R2TP would accompany clients from Hsp70 to Hsp90 to facilitate their assembly into macromolecular complexes.
    Title: Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables Hsp70 protein binding ISS
Inferred from Sequence or Structural Similarity
more info
  
enables Hsp90 protein binding ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
involved_in negative regulation of motor neuron apoptotic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of locomotor rhythm IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm ISS
Inferred from Sequence or Structural Similarity
more info
  

General protein information

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Preferred Names
spaghetti
Names
CG13570-PA
spag-PA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033778.4 Reference assembly

    Range
    24159384..24161503
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_079925.3 → NP_524664.1  spaghetti [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_524664.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q86PC1, Q9V3E9
    Related
    FBpp0072164
    Conserved Domains (6) summary
    smart00028
    Location:130 → 160
    TPR; Tetratricopeptide repeats
    sd00006
    Location:96 → 124
    TPR; TPR repeat [structural motif]
    pfam00515
    Location:102 → 129
    TPR_1; Tetratricopeptide repeat
    pfam13414
    Location:95 → 161
    TPR_11; TPR repeat
    pfam13432
    Location:136 → 197
    TPR_16; Tetratricopeptide repeat
    pfam13877
    Location:415 → 503
    RPAP3_C; Potential Monad-binding region of RPAP3
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q86PC1 Q86PC1
Q9V3E9 Q9V3E9

Gene LinkOut

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