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Pep Protein on ecdysone puffs [ Drosophila melanogaster (fruit fly) ]

Gene ID: 45961, updated on 2-Nov-2024

Summary

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Official Symbol
Pepprovided by FlyBase
Official Full Name
Protein on ecdysone puffsprovided by FlyBase
Primary source
FLYBASE:FBgn0004401
Locus tag
Dmel_CG6143
See related
AllianceGenome:FB:FBgn0004401
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG6143; Dmel\CG6143; p100; pep; PEP; PEP/X4; X4/PEP
Summary
Enables single-stranded DNA binding activity and single-stranded RNA binding activity. Involved in regulation of mRNA splicing, via spliceosome. Part of catalytic step 2 spliceosome and precatalytic spliceosome. Colocalizes with polytene chromosome puff. Is expressed in adult head and adult heart. Used to study alcohol use disorder. Orthologous to human CIZ1 (CDKN1A interacting zinc finger protein 1). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs
all
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Genomic context

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See Pep in Genome Data Viewer
Location:
74E4-74E4; 3-45 cM
Exon count:
5
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (17638103..17645036, complement)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (17631203..17638136, complement)

Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene long non-coding RNA:CR44567 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene Nedd4 family interacting protein Neighboring gene Keren

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A unique zinc finger protein is associated preferentially with active ecdysone-responsive loci in Drosophila. Amero SA, et al. Genes Dev, 1991 Feb. PMID 1899840
  2. Cloning of a gene localized and expressed at the ecdysteroid regulated puff 74EF in salivary glands of Drosophila larvae. Möritz T, et al. EMBO J, 1984 Feb. PMID 16453498, Free PMC Article
  3. Measurement of antigen by enhanced chemiluminescent western blot. Huang D, et al. Biotechniques, 1997 Mar. PMID 9067021
  4. A unique ribonucleoprotein complex assembles preferentially on ecdysone-responsive sites in Drosophila melanogaster. Amero SA, et al. Mol Cell Biol, 1993 Sep. PMID 8355684, Free PMC Article
  5. Structures and dynamics of Drosophila Tpr inconsistent with a static, filamentous structure. Zimowska G, et al. Exp Cell Res, 2002 Jun 10. PMID 12027452

See all (85) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables single-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables single-stranded RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in mRNA splicing, via spliceosome IC
Inferred by Curator
more info
 PubMed 
involved_in regulation of mRNA splicing, via spliceosome IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of catalytic step 2 spliceosome HDA PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
colocalizes_with polytene chromosome puff IDA
Inferred from Direct Assay
more info
 PubMed 
part_of precatalytic spliceosome HDA PubMed 

General protein information

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Preferred Names
protein on ecdysone puffs
Names
74F gene
CG6143-PA
CG6143-PB
CG6143-PC
CG6143-PD
Pep-PA
Pep-PB
Pep-PC
Pep-PD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_037436.4 Reference assembly

    Range
    17638103..17645036 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001259888.3 → NP_001246817.1  protein on ecdysone puffs, isoform D [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001246817.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    D5AEL0, M9NG39
    Conserved Domains (2) summary
    sd00020
    Location:267 → 287
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam12171
    Location:193 → 217
    zf-C2H2_jaz; Zinc-finger double-stranded RNA-binding

  2. NM_206396.4 → NP_996118.1  protein on ecdysone puffs, isoform C [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_996118.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    D5AEL0, M9NG39
    Conserved Domains (2) summary
    sd00020
    Location:267 → 287
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam12171
    Location:193 → 217
    zf-C2H2_jaz; Zinc-finger double-stranded RNA-binding

  3. NM_080119.6 → NP_524858.1  protein on ecdysone puffs, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_524858.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P41073, Q8IGB1, Q8MSV5, Q9VVJ2, Q9VVJ3, Q9VVJ4
    UniProtKB/TrEMBL
    D5AEL0
    Related
    FBpp0074963
    Conserved Domains (2) summary
    sd00020
    Location:290 → 310
    ZF_C2H2; C2H2 Zn finger [structural motif]
    pfam12171
    Location:216 → 240
    zf-C2H2_jaz; Zinc-finger double-stranded RNA-binding

  4. NM_168742.4 → NP_730290.3  protein on ecdysone puffs, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_730290.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P41073
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
M9NG39 M9NG39
P41073.1 P41073

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