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tisB membrane-depolarizing toxin TisB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 5061527, updated on 3-Dec-2024

Summary

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Official Symbol
tisB
Official Full Name
membrane-depolarizing toxin TisB
Primary source
ECOCYC:G0-9962
Locus tag
b4618
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4427; ysdB
Summary
A ~400 bp LexA-regulated mRNA covering tisB has been observed in Northern blots (De Henestrosa et al, 2000). lstR-1 mediates antisense inhibition of TisB translation and this inhibition may be mitigated during the SOS response by the the istR-2 sRNA which has a LexA-regulated promoter upstream of the IstR-1 promtoer (Vogel, 2004). [More information is available at EcoGene: EG14402]. The tisAB locus encodes the toxic peptide TisB. [More information is available at EcoCyc: G0-9962].
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Genomic context

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Sequence:
NC_000913.3 (3853553..3853642)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ilvBN operon leader peptide Neighboring gene ncRNA Neighboring gene protein YsdE Neighboring gene multidrug efflux pump EmrD

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Two regulatory RNA elements affect TisB-dependent depolarization and persister formation. Berghoff BA, et al. Mol Microbiol, 2017 Mar. PMID 27997707
  2. TisB Protein Protects Escherichia coli Cells Suffering Massive DNA Damage from Environmental Toxic Compounds. Su WL, et al. mBio, 2022 Apr 26. PMID 35377167, Free PMC Article
  3. Ciprofloxacin causes persister formation by inducing the TisB toxin in Escherichia coli. Dörr T, et al. PLoS Biol, 2010 Feb 23. PMID 20186264, Free PMC Article
  4. An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli. Romilly C, et al. Nucleic Acids Res, 2020 Dec 2. PMID 33231643, Free PMC Article
  5. The small RNA IstR inhibits synthesis of an SOS-induced toxic peptide. Vogel J, et al. Curr Biol, 2004 Dec 29. PMID 15620655

See all (19) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli.
    Title: An RNA pseudoknot is essential for standby-mediated translation of the tisB toxin mRNA in Escherichia coli.
  2. Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli.
    Title: Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli.
  3. The RNA antitoxin IstR-1 sets a threshold for TisB-dependent depolarization under DNA-damaging conditions, resulting in two sub-populations: polarized and depolarized cells.
    Title: Two regulatory RNA elements affect TisB-dependent depolarization and persister formation.
  4. TisB forms clusters of narrow pores that are essential for its mechanism of action
    Title: Persister-promoting bacterial toxin TisB produces anion-selective pores in planar lipid bilayers.
  5. TisB is an SOS-induced toxin that targets the inner membrane and causes cell death. It does not affect translation or transcription directly.
    Title: A small SOS-induced toxin is targeted against the inner membrane in Escherichia coli.
  6. Overexpression of tisAB is necessary for cleavage of the uxaA mRNA, encoding altronate dehydratase, an enzyme specific for feeding the sugar acid galacturonate into the Entner-Doudoroff pathway.
    Title: Overexpression of the LexA-regulated tisAB RNA in E. coli inhibits SOS functions; implications for regulation of the SOS response.
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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables monoatomic anion channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables monoatomic anion channel activity IEA
Inferred from Electronic Annotation
more info
  
enables monoatomic anion channel activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in SOS response IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within SOS response IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within SOS response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in dormancy process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within dormancy process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in monoatomic anion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
membrane-depolarizing toxin TisB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3853553..3853642
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. YP_001165331.1 membrane-depolarizing toxin TisB [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for YP_001165331.1

    Status: PROVISIONAL

    Conserved Domains (1) summary
    TIGR04459
    Location:1 → 29
    TisB_tox; type I toxin-antitoxin system toxin TisB
Nucleotide Protein
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