U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Slc9a8 solute carrier family 9 (sodium/hydrogen exchanger), member 8 [ Mus musculus (house mouse) ]

Gene ID: 77031, updated on 27-Nov-2024

Summary

Go to the top of the page Help
Official Symbol
Slc9a8provided by MGI
Official Full Name
solute carrier family 9 (sodium/hydrogen exchanger), member 8provided by MGI
Primary source
MGI:MGI:1924281
See related
Ensembl:ENSMUSG00000039463 AllianceGenome:MGI:1924281
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
NHE8; NHE-8; 1200006P13Rik; 6430709P13Rik
Summary
This gene encodes a member of the Na+/H+ exchanger (NHE) family of integral membrane transporter proteins. The encoded protein is expressed on the apical membrane of the intestinal epithelial cells and plays an important role in mucosal protection. Loss of the encoded protein in mice results in a decrease in the number of goblet and mucin-positive cells, disorganization of the mucin layer, and a decrease in mucosal pH in the colon. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Expression
Ubiquitous expression in kidney adult (RPKM 45.2), adrenal adult (RPKM 11.3) and 28 other tissues See more
Orthologs
human all
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See Slc9a8 in Genome Data Viewer
Location:
2 H3; 2 87.22 cM
Exon count:
19
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (167261678..167318920)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (167421599..167477000)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene prostaglandin I2 (prostacyclin) synthase Neighboring gene predicted gene, 52569 Neighboring gene UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 Neighboring gene STARR-positive B cell enhancer ABC_E2061 Neighboring gene STARR-positive B cell enhancer ABC_E7887 Neighboring gene STARR-positive B cell enhancer ABC_E419 Neighboring gene STARR-positive B cell enhancer mm9_chr2:167200540-167200841 Neighboring gene STARR-positive B cell enhancer ABC_E4522 Neighboring gene STARR-seq mESC enhancer starr_06429 Neighboring gene STARR-positive B cell enhancer ABC_E7888 Neighboring gene STARR-seq mESC enhancer starr_06433 Neighboring gene STARR-positive B cell enhancer ABC_E9075 Neighboring gene spermatogenesis associated 2 Neighboring gene ring finger protein 114 Neighboring gene STARR-seq mESC enhancer starr_06435 Neighboring gene predicted gene 11474

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help
  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. An indisputable role of NHE8 in mucosal protection. Bernardazzi C, et al. Am J Physiol Gastrointest Liver Physiol, 2020 Oct 1. PMID 32755385, Free PMC Article
  2. NHE8 plays important roles in gastric mucosal protection. Xu H, et al. Am J Physiol Gastrointest Liver Physiol, 2013 Feb 1. PMID 23220221, Free PMC Article
  3. NHE8 plays an important role in mucosal protection via its effect on bacterial adhesion. Liu C, et al. Am J Physiol Cell Physiol, 2013 Jul 1. PMID 23657568, Free PMC Article
  4. Glucocorticoids Reduce Renal NHE8 Expression. Joseph C, et al. Physiol Rep, 2013 Aug 1. PMID 24032075, Free PMC Article
  5. Loss of NHE8 expression impairs ocular surface function in mice. Xu H, et al. Am J Physiol Cell Physiol, 2015 Jan 1. PMID 25377091, Free PMC Article

See all (47) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. An indisputable role of NHE8 in mucosal protection.
    Title: An indisputable role of NHE8 in mucosal protection.
  2. NHE8 regulates Ca(2+) uptake into the proximal tubule epithelium.
    Title: NHE8 attenuates Ca(2+) influx into NRK cells and the proximal tubule epithelium.
  3. NHE8 deficiency resulted in increased Lgr5 expression in the colon.
    Title: NHE8 Deficiency Promotes Colitis-Associated Cancer in Mice via Expansion of Lgr5-Expressing Cells.
  4. These results indicate that NHE8 plays essential function in both retinal pigment epithelium (RPE) and photoreceptor cells. NHE8 dysfunction either in photoreceptor or RPE is sufficient to cause retinal degeneration in adult mice at any age.
    Title: Essential function of NHE8 in mouse retina demonstrated by AAV-mediated CRISPR/Cas9 knockdown.
  5. work has uncovered a crucial role of NHE8 in acrosome biogenesis and suggests that some forms of human globozoospermia might be caused by a loss of function of this Na(+)/H(+) exchanger.
    Title: Loss of the Na(+)/H(+) exchanger NHE8 causes male infertility in mice by disrupting acrosome formation.
  6. NHE8 may be involved in the establishment of intestinal mucosal integrity by regulating the functions of goblet and Paneth cells.
    Title: Loss of NHE8 expression impairs intestinal mucosal integrity.
  7. NHE8 function is necessary for the survival of photoreceptor cells and NHE8 is important for RPE cell polarity and function
    Title: NHE8 is essential for RPE cell polarity and photoreceptor survival.
  8. The NHE8 protein is required in the RPE to maintain correct regulation of endosomal volume and/or pH which is essential for the cellular integrity and subsequent function of RPE
    Title: Mouse slc9a8 mutants exhibit retinal defects due to retinal pigmented epithelium dysfunction.
  9. Suggest a novel role of NHE8 in male fertility through its effect on modifying luteinizing hormone receptor function.
    Title: Disruption of NHE8 expression impairs Leydig cell function in the testes.
  10. Both NHE3 and NHE8 likely play a role in neonatal acidification.
    Title: Renal NHE expression and activity in neonatal NHE3- and NHE8-null mice.
Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables potassium:proton antiporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables potassium:proton antiporter activity IEA
Inferred from Electronic Annotation
more info
  
enables potassium:proton antiporter activity ISO
Inferred from Sequence Orthology
more info
  
enables sodium:proton antiporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sodium:proton antiporter activity ISO
Inferred from Sequence Orthology
more info
  
enables sodium:proton antiporter activity ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
involved_in acrosome assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in potassium ion transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in potassium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proton transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proton transmembrane transport ISO
Inferred from Sequence Orthology
more info
  
involved_in proton transmembrane transport ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of Golgi lumen acidification IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of Golgi lumen acidification ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of intracellular pH IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of intracellular pH IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of intracellular pH ISO
Inferred from Sequence Orthology
more info
  
involved_in sodium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in sodium ion transmembrane transport ISO
Inferred from Sequence Orthology
more info
  
involved_in sodium ion transmembrane transport ISS
Inferred from Sequence or Structural Similarity
more info
  
Component Evidence Code Pubs
located_in Golgi membrane ISO
Inferred from Sequence Orthology
more info
  
located_in Golgi membrane ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in acrosomal vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in apical plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in apical plasma membrane ISO
Inferred from Sequence Orthology
more info
  
located_in multivesicular body membrane IEA
Inferred from Electronic Annotation
more info
  
located_in multivesicular body membrane ISO
Inferred from Sequence Orthology
more info
  
located_in trans-Golgi network membrane IEA
Inferred from Electronic Annotation
more info
  
located_in trans-Golgi network membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

Go to the top of the page Help
Preferred Names
sodium/hydrogen exchanger 8
Names
Na(+)/H(+) exchanger 8
sodium/proton exchanger NHE8

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001304540.1 → NP_001291469.1  sodium/hydrogen exchanger 8 isoform c

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform c, which has a shorter N-terminus compared to isoform a.
    Source sequence(s)
    AK049410, AL591762, BC058947
    UniProtKB/TrEMBL
    Q69ZU9
    Related
    ENSMUST00000131956.8
    Conserved Domains (1) summary
    COG0025
    Location:21 → 386
    NhaP; NhaP-type Na+/H+ or K+/H+ antiporter [Inorganic ion transport and metabolism]

  2. NM_001304542.1 → NP_001291471.1  sodium/hydrogen exchanger 8 isoform d

    See identical proteins and their annotated locations for NP_001291471.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an alternate exon in the 5' coding region and uses an alternate in-frame splice site internally, compared to variant 1. The encoded isoform (d) is shorter, compared to isoform a.
    Source sequence(s)
    AK051791, AL591762, BC058947
    Consensus CDS
    CCDS84586.1
    UniProtKB/TrEMBL
    A2A464, Q69ZU9
    Related
    ENSMUSP00000104841.2, ENSMUST00000109218.7
    Conserved Domains (1) summary
    COG0025
    Location:57 → 449
    NhaP; NhaP-type Na+/H+ or K+/H+ antiporter [Inorganic ion transport and metabolism]

  3. NM_148929.3 → NP_683731.1  sodium/hydrogen exchanger 8 isoform a

    See identical proteins and their annotated locations for NP_683731.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (a).
    Source sequence(s)
    AK049410, AL591762, BC058947
    Consensus CDS
    CCDS38339.1
    UniProtKB/Swiss-Prot
    A2A465, Q3UPR4, Q5WA59, Q8BIH8, Q8BJ27, Q8R4D1
    UniProtKB/TrEMBL
    Q69ZU9
    Related
    ENSMUSP00000044185.7, ENSMUST00000047815.13
    Conserved Domains (1) summary
    COG0025
    Location:55 → 480
    NhaP; NhaP-type Na+/H+ or K+/H+ antiporter [Inorganic ion transport and metabolism]

RNA

  1. NR_130767.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) lacks three alternate internal exons, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AK160826, AL589870, AL591762, BC058947
    Related
    ENSMUST00000139673.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    167261678..167318920
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036162699.1 → XP_036018592.1  sodium/hydrogen exchanger 8 isoform X1

    Conserved Domains (1) summary
    TIGR00840
    Location:59 → 483
    b_cpa1; sodium/hydrogen exchanger 3

  2. XM_036162701.1 → XP_036018594.1  sodium/hydrogen exchanger 8 isoform X3

    UniProtKB/TrEMBL
    Q69ZU9
    Conserved Domains (1) summary
    TIGR00840
    Location:2 → 369
    b_cpa1; sodium/hydrogen exchanger 3

  3. XM_036162706.1 → XP_036018599.1  sodium/hydrogen exchanger 8 isoform X6

    Conserved Domains (1) summary
    TIGR00840
    Location:27 → 282
    b_cpa1; sodium/hydrogen exchanger 3

  4. XM_036162705.1 → XP_036018598.1  sodium/hydrogen exchanger 8 isoform X5

    Conserved Domains (1) summary
    TIGR00840
    Location:1 → 357
    b_cpa1; sodium/hydrogen exchanger 3

  5. XM_036162704.1 → XP_036018597.1  sodium/hydrogen exchanger 8 isoform X5

    Conserved Domains (1) summary
    TIGR00840
    Location:1 → 357
    b_cpa1; sodium/hydrogen exchanger 3

  6. XM_036162700.1 → XP_036018593.1  sodium/hydrogen exchanger 8 isoform X2

    UniProtKB/TrEMBL
    Q69ZU9
    Conserved Domains (1) summary
    TIGR00840
    Location:5 → 429
    b_cpa1; sodium/hydrogen exchanger 3

  7. XM_036162707.1 → XP_036018600.1  sodium/hydrogen exchanger 8 isoform X6

    Conserved Domains (1) summary
    TIGR00840
    Location:27 → 282
    b_cpa1; sodium/hydrogen exchanger 3

  8. XM_036162702.1 → XP_036018595.1  sodium/hydrogen exchanger 8 isoform X4

    Conserved Domains (1) summary
    TIGR00840
    Location:44 → 429
    b_cpa1; sodium/hydrogen exchanger 3

  9. XM_036162708.1 → XP_036018601.1  sodium/hydrogen exchanger 8 isoform X6

    Related
    ENSMUST00000148454.2
    Conserved Domains (1) summary
    TIGR00840
    Location:27 → 282
    b_cpa1; sodium/hydrogen exchanger 3

RNA

  1. XR_001783205.3 RNA Sequence

  2. XR_004940773.1 RNA Sequence

  3. XR_004940774.1 RNA Sequence

  4. XR_004940775.1 RNA Sequence

  5. XR_004940776.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_178371.3: Suppressed sequence

    Description
    NM_178371.3: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8R4D1.1 GenPept UniProtKB/Swiss-Prot:Q8R4D1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous