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AAT aspartate aminotransferase [ Arabidopsis thaliana (thale cress) ]

Gene ID: 816758, updated on 18-Sep-2024

Summary

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Official Symbol
AAT
Official Full Name
aspartate aminotransferase
Primary source
TAIR:AT2G22250
Locus tag
AT2G22250
See related
Araport:AT2G22250
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
aspartate aminotransferase; ASPARTATE AMINOTRANSFERASE; ATAAT; MATERNAL EFFECT EMBRYO ARREST 17; MEE17; T26C19.9; T26C19_9
Summary
Encodes a prokaryotic-type plastidic aspartate aminotransferase with glutamate/aspartate-prephenate aminotransferase (PAT) activity.
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Genomic context

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See AAT in Genome Data Viewer
Location:
chromosome: 2
Exon count:
11
Sequence:
Chromosome: 2; NC_003071.7 (9457786..9460919, complement)

Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Thioesterase superfamily protein Neighboring gene myo-inositol-1-phosphate synthase 2 Neighboring gene uncharacterized protein Neighboring gene oxidoreductase, 2OG-Fe(II) oxygenase family protein Neighboring gene hematological/neurological-like protein Neighboring gene tRNA-Arg

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Prephenate aminotransferase directs plant phenylalanine biosynthesis via arogenate. Maeda H, et al. Nat Chem Biol, 2011 Jan. PMID 21102469
  2. Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesis. Graindorge M, et al. FEBS Lett, 2010 Oct 22. PMID 20883697
  3. Tyrosine metabolism: identification of a key residue in the acquisition of prephenate aminotransferase activity by 1β aspartate aminotransferase. Giustini C, et al. FEBS J, 2019 Jun. PMID 30771275
  4. Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis. Pagnussat GC, et al. Development, 2005 Feb. PMID 15634699
  5. Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism. Ito J, et al. J Proteome Res, 2011 Apr 1. PMID 21166475

See all (9) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. X-ray crystal structure analysis supported by site-directed mutagenesis, modeling, and molecular dynamics simulations identified a molecular determinant of prephenate aminotransferase activity in the flexible N-terminal loop of 1beta AAT. A Lys/Arg/Gln residue in position 12 in the sequence present only in PAT-competent enzymes could interact with the 4-hydroxyl group of the prephenate substrate.
    Title: Tyrosine metabolism: identification of a key residue in the acquisition of prephenate aminotransferase activity by 1β aspartate aminotransferase.
  2. Data show that the purifies prephenate aminotransferase activity is housed by the prokaryotic-type plastidic aspartate aminotransferase (At2g22250).
    Title: Identification of a plant gene encoding glutamate/aspartate-prephenate aminotransferase: the last homeless enzyme of aromatic amino acids biosynthesis.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

NM_001036317.1
NM_127791.3
NM_179691.4

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables L-aspartate:2-oxoglutarate aminotransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables aspartate-prephenate aminotransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutamate-prephenate aminotransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-phenylalanine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within aromatic amino acid family biosynthetic process, prephenate pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in aromatic amino acid family biosynthetic process, prephenate pathway IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within embryo development ending in seed dormancy IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in chloroplast HDA PubMed 
located_in chloroplast ISM
Inferred from Sequence Model
more info
  
located_in chloroplast stroma HDA PubMed 
NOT located_in cytosol RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 

General protein information

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Preferred Names
aspartate aminotransferase
NP_001031394.1
  • aspartate aminotransferase (AAT); FUNCTIONS IN: L-aspartate:2-oxoglutarate aminotransferase activity, transferase activity, transferring nitrogenous groups, pyridoxal phosphate binding, catalytic activity; INVOLVED IN: embryo development ending in seed dormancy; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferases, class-I, pyridoxal-phosphate-binding site (InterPro:IPR004838), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT1G77670.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
NP_565529.1
  • aspartate aminotransferase (AAT); FUNCTIONS IN: L-aspartate:2-oxoglutarate aminotransferase activity, transferase activity, transferring nitrogenous groups, pyridoxal phosphate binding, catalytic activity; INVOLVED IN: embryo development ending in seed dormancy; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferases, class-I, pyridoxal-phosphate-binding site (InterPro:IPR004838), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT1G77670.1); Has 50315 Blast hits to 50311 proteins in 3104 species: Archae - 1094; Bacteria - 34657; Metazoa - 783; Fungi - 895; Plants - 1393; Viruses - 0; Other Eukaryotes - 11493 (source: NCBI BLink).
NP_850022.1
  • aspartate aminotransferase (AAT); FUNCTIONS IN: L-aspartate:2-oxoglutarate aminotransferase activity, pyridoxal phosphate binding, transferase activity, transferring nitrogenous groups, catalytic activity; INVOLVED IN: embryo development ending in seed dormancy; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferases, class-I, pyridoxal-phosphate-binding site (InterPro:IPR004838), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT1G77670.1); Has 49853 Blast hits to 49849 proteins in 3081 species: Archae - 1094; Bacteria - 34200; Metazoa - 783; Fungi - 895; Plants - 1391; Viruses - 0; Other Eukaryotes - 11490 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003071.7 Reference assembly

    Range
    9457786..9460919 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_179691.4 → NP_850022.1  aspartate aminotransferase [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_850022.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VZ85
    Conserved Domains (2) summary
    cd00609
    Location:55 → 417
    AAT_like; Aspartate aminotransferase family. This family belongs to pyridoxal phosphate (PLP)-dependent aspartate aminotransferase superfamily (fold I). Pyridoxal phosphate combines with an alpha-amino acid to form a compound called a Schiff base or aldimine ...
    PRK05764
    Location:23 → 423
    PRK05764; aspartate aminotransferase; Provisional

  2. NM_127791.3 → NP_565529.1  aspartate aminotransferase [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_565529.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    E9L7A7, Q3E6N9, Q8LFX1, Q8VZI1, Q9SIE1
    UniProtKB/TrEMBL
    A0A178VZ85, A0A5S9X058, A0A654EW86
    Conserved Domains (1) summary
    PRK05764
    Location:70 → 470
    PRK05764; aspartate aminotransferase; Provisional

  3. NM_001036317.1 → NP_001031394.1  aspartate aminotransferase [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_001031394.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    E9L7A7, Q3E6N9, Q8LFX1, Q8VZI1, Q9SIE1
    UniProtKB/TrEMBL
    A0A178VZ85, A0A5S9X058, A0A654EW86
    Conserved Domains (1) summary
    PRK05764
    Location:70 → 470
    PRK05764; aspartate aminotransferase; Provisional
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9SIE1.2

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