CRN Protein kinase superfamily protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

Go to the top of the page Help

Official Symbol: CRN
Official Full Name: Protein kinase superfamily protein
Primary source: TAIR:AT5G13290
Locus tag: AT5G13290
See related: Araport:AT5G13290
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: CORYNE; SOL2; SUPPRESSOR OF LLP1 2; T31B5.110; T31B5_110
Summary: Encodes a protein with predicted Ser/Thr kinase activity and membrane localization that is involved in the CLV3 signaling pathway that represses WUS expression in the meristem. Loss of function of CRN can suppress the phenotype caused by overexpression of CLV3. SOL2 isolated as a suppressor of root- specific overexpression of CLE19, a clavata3 like gene. sol2 partially suppresses the short root phenotype caused by CLE19 overexpression. Mutant flowers have extra carpels.
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See CRN in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 2

Sequence:: Chromosome: 5; NC_003076.8 (4252842..4255378, complement)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CLAVATA Signaling Ensures Reproductive Development in Plants across Thermal Environments.
Title: CLAVATA Signaling Ensures Reproductive Development in Plants across Thermal Environments.
SOL1 and SOL2 regulate fate transition and cell divisions in the Arabidopsis stomatal lineage.
Title: SOL1 and SOL2 regulate fate transition and cell divisions in the Arabidopsis stomatal lineage.
study reports that the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) are necessary to fully sense root-active CLE peptides; CRN stabilizes BAM3 expression and thus is required for BAM3-mediated CLE45 signaling.
Title: Perception of root-active CLE peptides requires CORYNE function in the phloem vasculature.
the class I phenotype can be mimicked by knocking out both TSO1 and its homolog SOL2 in double mutants.
Title: Recessive antimorphic alleles overcome functionally redundant loci to reveal TSO1 function in Arabidopsis flowers and meristems.
Our results suggest a model in which CLV1/BAM1 and CLV2/CRN complexes act in separate, parallel pathways in shoot meristems, while the CLV1, CLV2, and CRN receptors function together in a linear pathway during fruit development.
Title: CLAVATA signaling pathway receptors of Arabidopsis regulate cell proliferation in fruit organ formation as well as in meristems.
A 12-mer random-peptide library was used to determine the specific amino acid sequences binding with the recombinant CRN molecule.
Title: Identification of target ligands of CORYNE in Arabidopsis by phage display library.
CRN contains features of a pseudokinase but lacks kinase activity
Title: An evolutionarily conserved pseudokinase mediates stem cell production in plants.
The results therefore imply that the CLE14/CLE20 peptides may act through the CLV2-CRN receptor kinase, and that their availabilities and/or abundances may be affected by cytokinin activity in planta.
Title: CLE14/CLE20 peptides may interact with CLAVATA2/CORYNE receptor-like kinases to irreversibly inhibit cell division in the root meristem of Arabidopsis.
Data show that CRN, rather than CLV1 and CLV2, was able to form homodimers without CLV3 stimulation.
Title: Analysis of interactions among the CLAVATA3 receptors reveals a direct interaction between CLAVATA2 and CORYNE in Arabidopsis.
This study suggested that the CLV3 signal is perceived by a tetrameric CLV2/CRN complex and a CLV1 homodimer that localize to the PM and can interact via CRN.
Title: Stem cell signaling in Arabidopsis requires CRN to localize CLV2 to the plasma membrane.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

NM_001085108.1

Supported by ESTs:

NM_121332.4

Supported by ESTs:

NM_180481.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables peptide receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
NOT enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables receptor serine/threonine kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling receptor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in maintenance of root meristem identity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within meristem maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phloem development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of flower development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of meristem growth	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of meristem growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of meristem growth	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of root meristem growth	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISM Inferred from Sequence Model more info
located_in plasma membrane	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: Protein kinase superfamily protein

NP_001078577.1

CORYNE (CRN); FUNCTIONS IN: protein kinase activity, kinase activity, ATP binding; INVOLVED IN: regulation of meristem growth, regulation of root meristem growth, meristem maintenance, regulation of flower development; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, catalytic domain (InterPro:IPR000719), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009); BEST Arabidopsis thaliana protein match is: Leucine-rich repeat protein kinase family protein (TAIR:AT2G31880.1); Has 55771 Blast hits to 55472 proteins in 1453 species: Archae - 44; Bacteria - 2468; Metazoa - 16951; Fungi - 4037; Plants - 25285; Viruses - 100; Other Eukaryotes - 6886 (source: NCBI BLink).

NP_196833.2

CORYNE (CRN); FUNCTIONS IN: protein kinase activity, kinase activity, ATP binding; INVOLVED IN: regulation of meristem growth, regulation of root meristem growth, meristem maintenance, regulation of flower development; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, catalytic domain (InterPro:IPR000719), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009); BEST Arabidopsis thaliana protein match is: Leucine-rich repeat protein kinase family protein (TAIR:AT2G31880.1); Has 90342 Blast hits to 89573 proteins in 2628 species: Archae - 105; Bacteria - 9194; Metazoa - 31524; Fungi - 7067; Plants - 29119; Viruses - 228; Other Eukaryotes - 13105 (source: NCBI BLink).

NP_850812.2

CORYNE (CRN); FUNCTIONS IN: protein kinase activity, kinase activity, ATP binding; INVOLVED IN: regulation of meristem growth, regulation of root meristem growth, meristem maintenance, regulation of flower development; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, catalytic domain (InterPro:IPR000719), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009); BEST Arabidopsis thaliana protein match is: Leucine-rich repeat protein kinase family protein (TAIR:AT2G31880.1); Has 92136 Blast hits to 91345 proteins in 2709 species: Archae - 107; Bacteria - 9592; Metazoa - 32171; Fungi - 7128; Plants - 29600; Viruses - 232; Other Eukaryotes - 13306 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

4252842..4255378 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_180481.4 → NP_850812.2 Protein kinase superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850812.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A8MRZ3, Q3E9I0, Q9LYU7

UniProtKB/TrEMBL

A0A5S9Y4A7, A0A654G0N8

Conserved Domains (2) summary

cd14066
Location:124 → 398

STKc_IRAK; Catalytic domain of the Serine/Threonine kinases, Interleukin-1 Receptor Associated Kinases and related STKs

pfam00069
Location:121 → 394

Pkinase; Protein kinase domain
NM_001085108.1 → NP_001078577.1 Protein kinase superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001078577.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9LYU7

Conserved Domains (1) summary

cl21453
Location:120 → 274

PKc_like; Protein Kinases, catalytic domain
NM_121332.4 → NP_196833.2 Protein kinase superfamily protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_196833.2

Status: REVIEWED

UniProtKB/TrEMBL

A0A5S9Y4A7, A0A654G0N8

Conserved Domains (1) summary

cd14066
Location:120 → 373

STKc_IRAK; Catalytic domain of the Serine/Threonine kinases, Interleukin-1 Receptor Associated Kinases and related STKs