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NIT4 nitrilase 4 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 832290, updated on 18-Sep-2024

Summary

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Official Symbol
NIT4
Official Full Name
nitrilase 4
Primary source
TAIR:AT5G22300
Locus tag
AT5G22300
See related
Araport:AT5G22300
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
AtNIT4; nitrilase 4; NITRILASE 4; T6G21.6
Summary
encodes a nitrilase isomer. The purified enzyme shows a strong substrate specificity for beta-cyano-L-alanine, a intermediate product of the cyanide detoxification pathway.
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Genomic context

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See NIT4 in Genome Data Viewer
Location:
chromosome: 5
Exon count:
5
Sequence:
Chromosome: 5; NC_003076.8 (7379187..7382025)

Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene peptidyl-prolyl cis-trans isomerase G Neighboring gene NAC domain containing protein 89 Neighboring gene trichohyalin-like protein Neighboring gene tRNA-Gln

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Cryo-EM and directed evolution reveal how Arabidopsis nitrilase specificity is influenced by its quaternary structure. Mulelu AE, et al. Commun Biol, 2019. PMID 31341959, Free PMC Article
  2. Increased β-cyanoalanine nitrilase activity improves cyanide tolerance and assimilation in Arabidopsis. O'Leary B, et al. Mol Plant, 2014 Jan. PMID 23825089
  3. The Arabidopsis thaliana isogene NIT4 and its orthologs in tobacco encode beta-cyano-L-alanine hydratase/nitrilase. Piotrowski M, et al. J Biol Chem, 2001 Jan 26. PMID 11060302
  4. Differential regulation of an auxin-producing nitrilase gene family in Arabidopsis thaliana. Bartel B, et al. Proc Natl Acad Sci U S A, 1994 Jul 5. PMID 8022831, Free PMC Article
  5. A proteomic strategy for global analysis of plant protein complexes. Aryal UK, et al. Plant Cell, 2014 Oct. PMID 25293756, Free PMC Article

See all (6) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. a loop between alpha2 and alpha3 limits the length of the binding pocket and propose that it shifts position as a function of helical twist
    Title: Cryo-EM and directed evolution reveal how Arabidopsis nitrilase specificity is influenced by its quaternary structure.
  2. role of beta-cyanoalanine nitrilase in cyanide tolerance and assimilation in Arabidopsis
    Title: Increased β-cyanoalanine nitrilase activity improves cyanide tolerance and assimilation in Arabidopsis.
Submit: New GeneRIF Correction

General gene information

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NM_122135.6

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables 3-cyanoalanine hydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cyanoalanine nitrilase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cyanoalanine nitrilase activity IEA
Inferred from Electronic Annotation
more info
  
NOT enables indole-3-acetonitrile nitrilase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables indole-3-acetonitrile nitrilase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables nitrilase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables nitrile hydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within cyanide metabolic process IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within detoxification of nitrogen compound IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol HDA PubMed 
located_in plasma membrane ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
nitrilase 4
NP_197622.1
  • nitrilase 4 (NIT4); CONTAINS InterPro DOMAIN/s: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase (InterPro:IPR003010), Nitrilase/cyanide hydratase, conserved site (InterPro:IPR000132); BEST Arabidopsis thaliana protein match is: nitrilase 3 (TAIR:AT3G44320.1); Has 7653 Blast hits to 7575 proteins in 1747 species: Archae - 162; Bacteria - 5078; Metazoa - 407; Fungi - 539; Plants - 330; Viruses - 11; Other Eukaryotes - 1126 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003076.8 Reference assembly

    Range
    7379187..7382025
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001343724.1 → NP_001330785.1  nitrilase 4 [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A1P8BBX1
    Conserved Domains (1) summary
    PLN02504
    Location:23 → 261
    PLN02504; nitrilase

  2. NM_122135.6 → NP_197622.1  nitrilase 4 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_197622.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P46011, Q8L9L4
    UniProtKB/TrEMBL
    A0A178U938, A0A654G378
    Conserved Domains (1) summary
    PLN02504
    Location:12 → 354
    PLN02504; nitrilase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P46011.1

Gene LinkOut

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