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ENDO2 endonuclease 2 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 843158, updated on 18-Sep-2024

Summary

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Official Symbol
ENDO2
Official Full Name
endonuclease 2
Primary source
TAIR:AT1G68290
Locus tag
AT1G68290
See related
Araport:AT1G68290
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
AtBFN2; endonuclease 2; T22E19.8; T22E19_8
Summary
Encodes an endonuclease ENDO2. ENDO2 purified from transgenic Arabidopsis digests RNA, ssDNA, and dsDNA, with a substrate preference for ssDNA and RNA. ENDO2 produced and purified from Nicotiana benthamiana expression showed no demonstrable endonuclease activity, either towards single stranded DNA or mismatches, in vitro.
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Genomic context

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See ENDO2 in Genome Data Viewer
Location:
chromosome: 1
Exon count:
9
Sequence:
Chromosome: 1; NC_003070.9 (25596671..25598438)

Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene Thioesterase superfamily protein Neighboring gene pseudo Neighboring gene ncRNA Neighboring gene Adenine nucleotide alpha hydrolases-like superfamily protein Neighboring gene MIP18 family protein (DUF59) Neighboring gene myb domain protein 62

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural insights of the ssDNA binding site in the multifunctional endonuclease AtBFN2 from Arabidopsis thaliana. Yu TF, et al. PLoS One, 2014. PMID 25157844, Free PMC Article
  2. Arabidopsis ENDO2: its catalytic role and requirement of N-glycosylation for function. Ko CY, et al. J Agric Food Chem, 2012 May 23. PMID 22506810
  3. The plant s1-like nuclease family has evolved a highly diverse range of catalytic capabilities. Lesniewicz K, et al. Plant Cell Physiol, 2013 Jul. PMID 23620482
  4. Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea. Triques K, et al. Plant J, 2007 Sep. PMID 17651368
  5. Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana. Theologis A, et al. Nature, 2000 Dec 14. PMID 11130712

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The first complete, ligand-free, AtBFN2 crystal structure, along with sulfate, phosphate and ssDNA co-crystal structures.
    Title: Structural insights of the ssDNA binding site in the multifunctional endonuclease AtBFN2 from Arabidopsis thaliana.
  2. Data indicate that five S1-like nucleases have been named as ENDO1 (BFN1, AT1G11190), ENDO2 (AT1G68290), ENDO3 (AT4G21590), ENDO4 (AT4G21585) and ENDO5 (AT4G21600).
    Title: The plant s1-like nuclease family has evolved a highly diverse range of catalytic capabilities.
  3. ENDO2 is a Ca2+/Mn2+-dependent nuclease, digesting ssDNA and RNA at neutral pH. The same enzyme is also able to digest dsDNA at acidic pH and in the simultaneous presence of Ca2+ and Zn2+ ions. The expression profile of ENDO2 gene suggests that it plays a role in the chalazal endosperm development.
    Title: The plant s1-like nuclease family has evolved a highly diverse range of catalytic capabilities.
  4. at neutral pH, endonuclease ENDO2 cleaves mismatch regions in heteroduplex DNA containing single base pair mismatches or insertion/deletion bases and can be applied to high-throughput detection of single base mutation
    Title: Arabidopsis ENDO2: its catalytic role and requirement of N-glycosylation for function.
Submit: New GeneRIF Correction

General gene information

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Homology

NM_105500.2

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables RNA endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
NOT enables T/G mismatch-specific endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables T/G mismatch-specific endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables double-stranded DNA endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
NOT enables single-stranded DNA endodeoxyribonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables single-stranded DNA endodeoxyribonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in extracellular region ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
endonuclease 2
NP_176996.1
  • endonuclease 2 (ENDO 2); FUNCTIONS IN: endonuclease activity, nucleic acid binding; INVOLVED IN: DNA catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C/P1 nuclease, core (InterPro:IPR008947), S1/P1 nuclease (InterPro:IPR003154); BEST Arabidopsis thaliana protein match is: endonuclease 4 (TAIR:AT4G21585.1); Has 730 Blast hits to 727 proteins in 207 species: Archae - 0; Bacteria - 188; Metazoa - 0; Fungi - 100; Plants - 121; Viruses - 2; Other Eukaryotes - 319 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003070.9 Reference assembly

    Range
    25596671..25598438
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_105500.2 → NP_176996.1  endonuclease 2 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_176996.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q8LCL6, Q9C9G4
    UniProtKB/TrEMBL
    A0A5S9WQP7, A0A654EM30
    Conserved Domains (1) summary
    pfam02265
    Location:28 → 290
    S1-P1_nuclease; S1/P1 Nuclease
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9C9G4.1

Gene LinkOut

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