U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

RSA4 Rsa4p [ Saccharomyces cerevisiae S288C ]

Gene ID: 850435, updated on 4-Jan-2025

Summary

Go to the top of the page Help
Official Symbol
RSA4
Official Full Name
Rsa4p
Primary source
SGD:S000000668
Locus tag
YCR072C
See related
AllianceGenome:SGD:S000000668; FungiDB:YCR072C; VEuPathDB:YCR072C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Involved in protein-RNA complex remodeling and ribosomal large subunit assembly. Located in nucleolus. Orthologous to human NLE1 (notchless homolog 1). [provided by Alliance of Genome Resources, Jan 2025]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See RSA4 in Genome Data Viewer
Location:
chromosome: III
Exon count:
1
Sequence:
Chromosome: III; NC_001135.5 (240805..242352, complement)

Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene peptidylprolyl isomerase family protein CPR4 Neighboring gene mitochondrial 54S ribosomal protein IMG2 Neighboring gene mitogen-activated protein kinase kinase kinase SSK22 Neighboring gene Sol2p

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits. de la Cruz J, et al. Nucleic Acids Res, 2005. PMID 16221974, Free PMC Article
  2. The MIDAS domain of AAA mechanoenzyme Mdn1 forms catch bonds with two different substrates. Mickolajczyk KJ, et al. Elife, 2022 Feb 11. PMID 35147499, Free PMC Article
  3. 60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle. Leidig C, et al. Nat Commun, 2014 Mar 24. PMID 24662372
  4. Systematic Analysis of Phosphatidylinositol-5-phosphate-Interacting Proteins Using Yeast Proteome Microarrays. Herianto S, et al. Anal Chem, 2021 Jan 19. PMID 33302626
  5. Architecture of the Rix1-Rea1 checkpoint machinery during pre-60S-ribosome remodeling. Barrio-Garcia C, et al. Nat Struct Mol Biol, 2016 Jan. PMID 26619264

See all (68) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Rsa4p provides a platform of interaction for correct processing of rRNA precursors and nucleolar release of 60S ribosomal subunits.
    Title: The essential WD-repeat protein Rsa4p is required for rRNA processing and intra-nuclear transport of 60S ribosomal subunits.
Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in protein-RNA complex remodeling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosomal large subunit assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ribosomal large subunit assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
Rsa4p
NP_009997.2
  • Ribosomal large subunit (60S) assembly factor; association of the N-terminal ubiquitin-like (UBL) domain of this preribosomal factor with the MIDAS domain in the Rea1p tail, promoted by the GTPase Nog2p, is required for the ATP-dependent dissociation of preribosomal factors including the Rix1p subcomplex, Rea1p, Nog2p and Rsa1p itself prior to nuclear export of pre-60S particles; contains WD-repeats and a UBL domain; localizes to the nucleolus

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001135.5 Reference assembly

    Range
    240805..242352 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178782.1 → NP_009997.2  TPA: Rsa4p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009997.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VR74, P25382, Q8NKJ4
    UniProtKB/TrEMBL
    A6ZTP5, B3LUC9, B5VF03, C7GUX4, C8Z4F9, G2WA81, N1P7P7
    Conserved Domains (4) summary
    smart00320
    Location:475 → 513
    WD40; WD40 repeats
    cd00200
    Location:135 → 472
    WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
    sd00039
    Location:189 → 227
    7WD40; WD40 repeat [structural motif]
    pfam08154
    Location:32 → 79
    NLE; NLE (NUC135) domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25382.3

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials
Tools