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MID2 Mid2p [ Saccharomyces cerevisiae S288C ]

Gene ID: 851042, updated on 9-Dec-2024

Summary

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Official Symbol
MID2
Official Full Name
Mid2p
Primary source
SGD:S000004324
Locus tag
YLR332W
See related
AllianceGenome:SGD:S000004324; FungiDB:YLR332W; VEuPathDB:YLR332W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
KAI1
Summary
Enables transmembrane signaling receptor activity. Involved in several processes, including IRE1-mediated unfolded protein response; pexophagy; and response to acidic pH. Located in plasma membrane. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MID2 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (790676..791806)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Rec102p Neighboring gene Chs5p Neighboring gene Jip3p Neighboring gene tRNA-Asp Neighboring gene ncRNA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The MID2 gene encodes a putative integral membrane protein with a Ca(2+)-binding domain and shows mating pheromone-stimulated expression in Saccharomyces cerevisiae. Ono T, et al. Gene, 1994 Dec 30. PMID 7828875
  2. Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast. Daniel J. Mol Gen Genet, 1993 Aug. PMID 8355657
  3. The effect of tea tree oil and antifungal agents on a reporter for yeast cell integrity signalling. Straede A, et al. Yeast, 2007 Apr. PMID 17397109
  4. Protein N-glycosylation determines functionality of the Saccharomyces cerevisiae cell wall integrity sensor Mid2p. Hutzler F, et al. Mol Microbiol, 2008 Jun. PMID 18410496
  5. Dissecting sensor functions in cell wall integrity signaling in Kluyveromyces lactis. Rodicio R, et al. Fungal Genet Biol, 2008 Apr. PMID 17827039

See all (115) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Signal sequence-independent targeting of MID2 mRNA to the endoplasmic reticulum by the yeast RNA-binding protein Khd1p.
    Title: Signal sequence-independent targeting of MID2 mRNA to the endoplasmic reticulum by the yeast RNA-binding protein Khd1p.
  2. The sensors Wsc1 and Mid2 reside in different plasma membrane compartments in Saccharomyces cerevisiae.
    Title: Yeast cell wall integrity sensors form specific plasma membrane microdomains important for signalling.
  3. The extent of the N-linked glycan and its distance from the plasma membrane affect Mid2p function, suggesting the N-glycan to be directly involved in Mid2p sensing.
    Title: Protein N-glycosylation determines functionality of the Saccharomyces cerevisiae cell wall integrity sensor Mid2p.
  4. Chimers of MID2 fused to GFP have been employed to show that intracellular and membrane distribution is only dependent on a membrane-anchored cytoplasmic tail.
    Title: Functional analyses of the extra- and intracellular domains of the yeast cell wall integrity sensors Mid2 and Wsc1.
  5. As the Mid2p amount plays a role in rgd1Delta sensitivity to low pH, Mid2p seems to act more like a molecular rheostat, controlling the level of PKC pathway activity and thus allowing phenotypical expression of RGD1 inactivation.
    Title: The Rgd1p Rho GTPase-activating protein and the Mid2p cell wall sensor are required at low pH for protein kinase C pathway activation and cell survival in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transmembrane signaling receptor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in IRE1-mediated unfolded protein response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in fungal-type cell wall organization IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in fungal-type cell wall organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pexophagy IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to acidic pH IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to osmotic stress IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in fungal-type vacuole HDA PubMed 
located_in mating projection tip HDA PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Mid2p
NP_013436.1
  • O-glycosylated plasma membrane protein; acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; MID2 has a paralog, MTL1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    790676..791806
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182221.1 → NP_013436.1  TPA: Mid2p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013436.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYX3, P36027, P41944
    UniProtKB/TrEMBL
    B3RHK6, B5VNM5, C7GUA4, C8ZDU3, G2WJF2
    Conserved Domains (1) summary
    pfam04478
    Location:179 → 312
    Mid2; Mid2 like cell wall stress sensor
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P36027.1

Gene LinkOut

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