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FLO1 flocculin FLO1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851289, updated on 9-Dec-2024

Summary

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Official Symbol
FLO1
Official Full Name
flocculin FLO1
Primary source
SGD:S000000084
Locus tag
YAR050W
See related
AllianceGenome:SGD:S000000084; FungiDB:YAR050W; VEuPathDB:YAR050W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
FLO2; FLO4
Summary
Enables D-mannose binding activity. Involved in flocculation. Located in fungal-type cell wall. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See FLO1 in Genome Data Viewer
Location:
chromosome: I
Exon count:
1
Sequence:
Chromosome: I; NC_001133.9 (203403..208016)

Chromosome I - NC_001133.9Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene oxysterol-binding protein related protein SWH1 Neighboring gene pseudo Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001133.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation-sedimentation. Gomes DG, et al. Bioresour Technol, 2012 Mar. PMID 22285899
  2. Construction of yeast strains with high cell surface lipase activity by using novel display systems based on the Flo1p flocculation functional domain. Matsumoto T, et al. Appl Environ Microbiol, 2002 Sep. PMID 12200308, Free PMC Article
  3. Long anchor using Flo1 protein enhances reactivity of cell surface-displayed glucoamylase to polymer substrates. Sato N, et al. Appl Microbiol Biotechnol, 2002 Dec. PMID 12466889
  4. [Cloning of flocculent gene and expression in industrial yeast strain]. Guo W, et al. Wei Sheng Wu Xue Bao, 2002 Feb. PMID 12557358
  5. [Effect of tandem repeats adjacent to 3'-terminal of FLO1 on the flocculation function of Saccharomyces cerevisiae]. Yue F, et al. Wei Sheng Wu Xue Bao, 2013 Dec 4. PMID 24697100

See all (96) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Sas3 and Ada2-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.
    Title: Sas3 and Ada2(Gcn5)-dependent histone H3 acetylation is required for transcription elongation at the de-repressed FLO1 gene.
  2. we identified a transposable element insertion in the promoter region of known flocculation gene FLO1, and, in an additional five clones, we recovered loss-of-function mutations in transcriptional repressor TUP1, which regulates FLO1 and other related genes
    Title: Experimental Evolution Reveals Favored Adaptive Routes to Cell Aggregation in Yeast.
  3. expression of FLO5 gene was higher than that of FLO1 gene
    Title: Genetic diversity of FLO1 and FLO5 genes in wine flocculent Saccharomyces cerevisiae strains.
  4. These data suggest that distinctly bound Cyc8-Tup1 cooperates with Hda1p and Rpd3p to establish or maintain an extensive array of strongly positioned, deacetylated nucleosomes over the FLO1 promoter and upstream region which inhibit histone acetylation, block Swi-Snf binding and prevent transcription.
    Title: The yeast Cyc8-Tup1 complex cooperates with Hda1p and Rpd3p histone deacetylases to robustly repress transcription of the subtelomeric FLO1 gene.
  5. Deletion of tandem repeats B, C and D adjacent to the 3'-terminal of FLO1 increases the conformation of flocculin in response to changes of pH and temperature.
    Title: [Effect of tandem repeats adjacent to 3'-terminal of FLO1 on the flocculation function of Saccharomyces cerevisiae].
  6. Expression of Lg-FLO1, which is required for flocculation of bottom-fermenting yeast, was also found to be induced by starvation for nitrogen.
    Title: Nitrogen starvation induces expression of Lg-FLO1 and flocculation in bottom-fermenting yeast.
  7. Repeated DNA sequence of FLO1 is important for the control of flocculation and genetic stability.
    Title: [Regulatory effect of FLO1 tandem repeats on the flocculation characteristics and genetic stability in Saccharomyces cerevisiae].
  8. N-terminal domain of Flo1p contains two mannose carbohydrate binding sites with different affinities
    Title: The N-terminal domain of the Flo1 flocculation protein from Saccharomyces cerevisiae binds specifically to mannose carbohydrates.
  9. FLONS, conferring NewFlo-type flocculation ability in yeast was cloned and The 3,396-bp ORF encoded a peptide of 1,132 amino acids with high identity to Flo1 protein
    Title: Deletion of tandem repeats causes flocculation phenotype conversion from Flo1 to NewFlo in Saccharomyces cerevisiae.
  10. The authors describe the discovery of an extracellular matrix produced by FLO1-expressing Saccharomyces cerevisiae cells.
    Title: Characterization of a biofilm-like extracellular matrix in FLO1-expressing Saccharomyces cerevisiae cells.
Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables D-mannose binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in flocculation IEA
Inferred from Electronic Annotation
more info
  
involved_in flocculation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cell periphery HDA PubMed 
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
located_in fungal-type cell wall IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in side of membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
flocculin FLO1
NP_009424.1
  • Lectin-like protein involved in flocculation; cell wall protein that binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; important for co-flocculation with other yeasts, mediating interaction with specific species; FLO1 has a paralog, FLO5, that arose from a segmental duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001133.9 Reference assembly

    Range
    203403..208016
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178230.1 → NP_009424.1  TPA: flocculin FLO1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009424.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A7U4Y7, D6VPN7, P32768, Q58HH7, Q58HH8, Q58HH9, Q58HI0
    Conserved Domains (2) summary
    pfam07691
    Location:100 → 249
    PA14; PA14 domain
    pfam13928
    Location:1235 → 1276
    Flocculin_t3; Flocculin type 3 repeat
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32768.4

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