ENA1 Na(+)/Li(+)-exporting P-type ATPase ENA1 [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: ENA1
Official Full Name: Na(+)/Li(+)-exporting P-type ATPase ENA1
Primary source: SGD:S000002447
Locus tag: YDR040C
See related: AllianceGenome:SGD:S000002447; FungiDB:YDR040C; VEuPathDB:YDR040C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HOR6; PMR2
Summary: Enables P-type potassium transmembrane transporter activity and P-type sodium transporter activity. Involved in several processes, including cellular response to glucose starvation; hyperosmotic response; and metal ion transport. Located in plasma membrane. [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See ENA1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (535192..538467, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Na(+) pump Ena1 is a yeast epsin-specific cargo requiring its ubiquitylation and phosphorylation sites for internalization.
Title: The Na(+) pump Ena1 is a yeast epsin-specific cargo requiring its ubiquitylation and phosphorylation sites for internalization.
Authors identify several HAL genes that act as multicopy suppressors of this sensitivity and are connected to the reduced function of the sodium ATPase Ena1.
Title: Involvement of the exomer complex in the polarized transport of Ena1 required for Saccharomyces cerevisiae survival against toxic cations.
Experimental data and mathematical models substantiate the existence of two stress-responsive Crz1-binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early response of the ENA1 promoter is about 60%.
Title: Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study.
These results show that, in response to salt stress, a functional Rim complex via specific ESCRT interactions is required for the proper accumulation of the Ena1 protein, but not induction of the ENA1 gene.
Title: A functional Rim101 complex is required for proper accumulation of the Ena1 Na+-ATPase protein in response to salt stress in Saccharomyces cerevisiae.
Data highlight a scenario in which the activities of Pho89 and Ena1 are functionally coordinated to sustain growth in an alkaline environment.
Title: Coregulated expression of the Na+/phosphate Pho89 transporter and Ena1 Na+-ATPase allows their functional coupling under high-pH stress.
All three potassium-exporting systems the Nha1 antiporter, Ena ATPase and Tok1 channel contribute to potassium homeostasis and are active upon potassium limitation in wild-type cells.
Title: Plasma-membrane hyperpolarization diminishes the cation efflux via Nha1 antiporter and Ena ATPase under potassium-limiting conditions.
analysis of regulation of the Saccharomyces cerevisiae ENA sodium ATPase system
Title: Function and regulation of the Saccharomyces cerevisiae ENA sodium ATPase system.
transcriptional response to alkaline stress of the ENA1 gene integrates three different signaling pathways
Title: The transcriptional response of the yeast Na(+)-ATPase ENA1 gene to alkaline stress involves three main signaling pathways.
Blocked exocytic sorting in sro7Delta mutants gives rise to quality control-mediated routing of Ena1p to the vacuole.
Title: The yeast tumor suppressor homologue Sro7p is required for targeting of the sodium pumping ATPase to the cell surface.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ABC-type sodium transporter activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled monoatomic cation transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables P-type ion transporter activity	IEA Inferred from Electronic Annotation more info
enables P-type potassium transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables P-type potassium transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables P-type sodium transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables P-type sodium transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables P-type sodium transporter activity	IEA Inferred from Electronic Annotation more info
enables P-type sodium transporter activity	IGI Inferred from Genetic Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to glucose starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in inorganic cation transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in intracellular calcium ion homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic cation transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in monoatomic cation transport	IEA Inferred from Electronic Annotation more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in potassium ion transport	IEA Inferred from Electronic Annotation more info
involved_in potassium ion transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pH	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to salt stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sodium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in sodium ion transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in sodium ion transport	IEA Inferred from Electronic Annotation more info
involved_in sodium ion transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cell periphery	HDA more info	PubMed
located_in fungal-type vacuole	HDA more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	HDA more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: Na(+)/Li(+)-exporting P-type ATPase ENA1

NP_010325.1

EC 7.2.2.3

P-type ATPase sodium pump; involved in Na+ and Li+ efflux to allow salt tolerance

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

535192..538467 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180348.1 → NP_010325.1 TPA: Na(+)/Li(+)-exporting P-type ATPase ENA1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010325.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VS28, P13587

UniProtKB/TrEMBL

A6ZY07

Conserved Domains (1) summary

TIGR01523
Location:11 → 1055

ATPase-IID_K-Na; potassium and/or sodium efflux P-type ATPase, fungal-type