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ALG3 dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase [ Saccharomyces cerevisiae S288C ]

Gene ID: 852196, updated on 4-Jan-2025

Summary

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Official Symbol
ALG3
Official Full Name
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase
Primary source
SGD:S000000178
Locus tag
YBL082C
See related
AllianceGenome:SGD:S000000178; FungiDB:YBL082C; VEuPathDB:YBL082C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
RHK1
Summary
Enables dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity. Involved in dolichol-linked oligosaccharide biosynthetic process. Located in endoplasmic reticulum. Used to study congenital disorder of glycosylation and congenital disorder of glycosylation Id. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Id. Orthologous to human ALG3 (ALG3 alpha-1,3- mannosyltransferase). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See ALG3 in Genome Data Viewer
Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (69751..71127, complement)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Boi1p Neighboring gene anaphase promoting complex subunit CDC27 Neighboring gene uncharacterized protein Neighboring gene glutamyl-tRNA(Gln) amidotransferase subunit PET112

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A novel yeast gene, RHK1, is involved in the synthesis of the cell wall receptor for the HM-1 killer toxin that inhibits beta-1,3-glucan synthesis. Kimura T, et al. Mol Gen Genet, 1997 Mar 26. PMID 9108275
  2. Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase. Sharma CB, et al. Biol Chem, 2001 Feb. PMID 11308030
  3. Large-scale isolation of dolichol-linked oligosaccharides with homogeneous oligosaccharide structures: determination of steady-state dolichol-linked oligosaccharide compositions. Kelleher DJ, et al. Glycobiology, 2001 Apr. PMID 11358881
  4. Deglycosylation systematically improves N-glycoprotein identification in liquid chromatography-tandem mass spectrometry proteomics for analysis of cell wall stress responses in Saccharomyces cerevisiae lacking Alg3p. Bailey UM, et al. J Chromatogr B Analyt Technol Biomed Life Sci, 2013 Apr 1. PMID 23454304
  5. The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing. Cipollo JF, et al. J Biol Chem, 2000 Feb 11. PMID 10660594

See all (60) citations in PubMed

GeneRIFs: Gene References Into Functions

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables mannosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in dolichol-linked oligosaccharide biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dolichol-linked oligosaccharide biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein glycosylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein glycosylation IEA
Inferred from Electronic Annotation
more info
  
involved_in protein glycosylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in endoplasmic reticulum HDA PubMed 
is_active_in endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase
NP_009471.1
  • Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    69751..71127 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178322.1 → NP_009471.1  TPA: dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009471.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VPS1, P38179
    UniProtKB/TrEMBL
    B3LNL4, B5VDQ9, C7GK52, C8Z3R2, G2W8T0, N1PAT6
    Conserved Domains (1) summary
    pfam05208
    Location:48 → 417
    ALG3; ALG3 protein
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38179.1

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