MPT5 Mpt5p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MPT5
Official Full Name: Mpt5p
Primary source: SGD:S000003146
Locus tag: YGL178W
See related: AllianceGenome:SGD:S000003146; FungiDB:YGL178W; VEuPathDB:YGL178W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HTR1; PUF5; UTH4
Summary: Enables mRNA binding activity and protein-macromolecule adaptor activity. Involved in intracellular mRNA localization; post-transcriptional regulation of gene expression; and regulation of G1 to G0 transition. Located in cytoplasm. [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See MPT5 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 2

Sequence:: Chromosome: VII; NC_001139.9 (167352..170571)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The RNA-binding protein Puf5 and the HMGB protein Ixr1 contribute to cell cycle progression through the regulation of cell cycle-specific expression of CLB1 in Saccharomyces cerevisiae.
Title: The RNA-binding protein Puf5 and the HMGB protein Ixr1 contribute to cell cycle progression through the regulation of cell cycle-specific expression of CLB1 in Saccharomyces cerevisiae.
The RNA-binding protein Puf5 contributes to buffering of mRNA upon chromatin-mediated changes in nascent transcription.
Title: The RNA-binding protein Puf5 contributes to buffering of mRNA upon chromatin-mediated changes in nascent transcription.
Crystal structures of Puf5p-RNA complexes reveal that the protein scaffold presents an exceptionally flat and extended interaction surface relative to other PUF proteins. In complexes with RNAs of different lengths, the protein is unchanged.
Title: RNA regulatory networks diversified through curvature of the PUF protein scaffold.
multiple Puf proteins coordinately regulate YHB1 mRNA to protect cells from nitric oxide stress.
Title: Conditional regulation of Puf1p, Puf4p, and Puf5p activity alters YHB1 mRNA stability for a rapid response to toxic nitric oxide stress in yeast.
Mpt5 represses the protein levels of two pathway components, the Ste7 MAP-kinase kinase and the Tec1 transcriptional activator, and negatively regulates the kinase activity of the Kss1 MAP kinase even in the absence of stimuli.
Title: Control of signaling in a MAP-kinase pathway by an RNA-binding protein.
mutations in BRR1 or MPT5 lead to a restoration of fertility and a more-or-less pronounced restoration of polarity; they also show genetic interactions with SSD1.
Title: Mutations in the Saccharomyces cerevisiae kinase Cbk1p lead to a fertility defect that can be suppressed by the absence of Brr1p or Mpt5p (Puf5p), proteins involved in RNA metabolism.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	IEA Inferred from Electronic Annotation more info
enables protein-macromolecule adaptor activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of translation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of translational initiation	IPI Inferred from Physical Interaction more info	PubMed
involved_in positive regulation of mRNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IDA Inferred from Direct Assay more info	PubMed
involved_in post-transcriptional regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in post-transcriptional regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in regulation of G1 to G0 transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of biological quality	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Mpt5p

NP_011337.1

mRNA-binding protein of the PUF family; binds to specific mRNAs, often in the 3' UTR; has broad specificity and binds to more than 1000 mRNAs (16% of the transcriptome); recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

167352..170571

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181043.1 → NP_011337.1 TPA: Mpt5p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011337.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTX5, P39016

UniProtKB/TrEMBL

C8Z874, G2WDS8

Conserved Domains (1) summary

COG5099
Location:49 → 848

COG5099; RNA-binding protein of the Puf family, translational repressor [Translation, ribosomal structure and biogenesis]