MLC1 Mlc1p [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: MLC1
Official Full Name: Mlc1p
Primary source: SGD:S000003074
Locus tag: YGL106W
See related: AllianceGenome:SGD:S000003074; FungiDB:YGL106W; VEuPathDB:YGL106W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables myosin II heavy chain binding activity and myosin V binding activity. Involved in protein localization to cell division site involved in mitotic actomyosin contractile ring assembly and vesicle targeting. Located in cellular bud neck contractile ring; cellular bud tip; and vesicle. Part of MIH complex and myosin II complex. Human ortholog(s) of this gene implicated in cardiomyopathy; congenital myopathy 14; familial atrial fibrillation; and hypertrophic cardiomyopathy 8. Orthologous to several human genes including MYL4 (myosin light chain 4); MYL6 (myosin light chain 6); and MYL6B (myosin light chain 6B). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See MLC1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (306560..307009)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Myo1 plays a major role in the septin-dependent Mlc1 localization to the division site before cytokinesis, whereas the formin Bni1 plays a major role in the actin filament-dependent Mlc1 localization during cytokinesis.
Title: Myosin‑II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis.
Here, we show that the rigid conformation of the loop connecting the two EF-hand motifs of the Mlc1p N-lobe explains its differential ability to interact with myosin V IQ1.
Title: N-lobe dynamics of myosin light chain dictates its mode of interaction with myosin V IQ1.
myosin light chain 1 (Mlc1p), required for vesicle delivery at the mother-bud neck during cytokinesis, binds to the Myo2p GTD in a region overlapping that of Ypt32p.
Title: Ypt32p and Mlc1p bind within the vesicle binding region of the class V myosin Myo2p globular tail domain.
Mlc1p/Myo2p interaction is stimulated by a signal that requires Sec2p and activation of Ypt32p
Title: GTP drives myosin light chain 1 interaction with the class V myosin Myo2 IQ motifs via a Sec2 RabGEF-mediated pathway.
NMR assignment of 1H, 13C and 15N resonances for Mlc1p from Saccharomices cerevisiae
Title: Assignment of the 1H, 13C and 15N resonances of Mlc1p from Saccharomices cerevisiae.
analysis of the interaction of Mlc1p with the first IQ motif of Myo2p
Title: Structural basis for the interaction of the myosin light chain Mlc1p with the myosin V Myo2p IQ motifs.
mrl1 is a mitochondrial factor for myo2p-dependent inheritance of mitochondria.
Title: Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast.
the 2-A resolution crystal structure of myosin light chain 1 (Mlc1p) bound to the IQ2-IQ3 fragment of Myo2p, a myosin V from Saccharomyces cerevisiae
Title: Structure of the light chain-binding domain of myosin V.

Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin II heavy chain binding	IPI Inferred from Physical Interaction more info	PubMed
enables myosin V binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in mitotic actomyosin contractile ring assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein localization to cell division site involved in mitotic actomyosin contractile ring assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein localization to cell division site involved in mitotic actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle targeting	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of MIH complex	IDA Inferred from Direct Assay more info	PubMed
part_of MIH complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IEA Inferred from Electronic Annotation more info
located_in cellular bud neck contractile ring	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IEA Inferred from Electronic Annotation more info
part_of myosin II complex	IBA Inferred from Biological aspect of Ancestor more info
part_of myosin II complex	IPI Inferred from Physical Interaction more info	PubMed
part_of myosin complex	IEA Inferred from Electronic Annotation more info
located_in site of polarized growth	IDA Inferred from Direct Assay more info	PubMed
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: Mlc1p

NP_011409.1

Essential light chain for Myo1p; light chain for Myo2p; stabilizes Myo2p by binding to the neck region; interacts with Myo1p, Iqg1p, and Myo2p to coordinate formation and contraction of the actomyosin ring with targeted membrane deposition

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

306560..307009

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180971.1 → NP_011409.1 TPA: Mlc1p [Saccharomyces cerevisiae S288C]

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU40, P53141

UniProtKB/TrEMBL

A6ZU75, B3LHH0, B5VIM0, C7GM01, C8Z8E2, G2WDZ4, N1P3I0

Conserved Domains (1) summary

COG5126
Location:1 → 149

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]