ALG2 GDP-Man:Man(1)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ALG2
Official Full Name: GDP-Man:Man(1)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase
Primary source: SGD:S000003033
Locus tag: YGL065C
See related: AllianceGenome:SGD:S000003033; FungiDB:YGL065C; VEuPathDB:YGL065C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity and glycolipid 1,6-alpha-mannosyltransferase activity. Involved in dolichol-linked oligosaccharide biosynthetic process. Located in endoplasmic reticulum. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Ii and congenital myasthenic syndrome 14. Orthologous to human ALG2 (ALG2 alpha-1,3/1,6-mannosyltransferase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ALG2 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (379760..381271, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The conserved C-terminal EX7E motif, N-terminal cytosolic tail, and 3 G-rich loop motifs in Alg2 play crucial roles for these activities, both in vitro and in vivo. These findings provide insight into the unique bifunctionality of Alg2 during LLO synthesis and lead to a new model in which alternative, independent routes exist for Alg2 catalysis of the trimannosyl core oligosaccharide.
Title: Alternative routes for synthesis of N-linked glycans by Alg2 mannosyltransferase.
EX(7)E motif, conserved in a variety of glycosyltransferases, is not important for Alg2 function in vivo and in vitro.
Title: Biochemical characterization and membrane topology of Alg2 from Saccharomyces cerevisiae as a bifunctional alpha1,3- and 1,6-mannosyltransferase involved in lipid-linked oligosaccharide biosynthesis.
The roles of Alg2 and Alg1 in the dolichol pathway were studied.
Title: In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables GDP-Man:Man1GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables alpha-1,3-mannosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glycolipid 1,6-alpha-mannosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables glycosyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in dolichol-linked oligosaccharide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dolichol-linked oligosaccharide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in protein glycosylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in endomembrane system	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: GDP-Man:Man(1)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase

NP_011450.1

EC 2.4.1.132

EC 2.4.1.257

Mannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in N-linked glycosylation pathway; mutants exhibit temperature-sensitive growth and abnormal accumulation of lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; mutations of human ALG2 cause congenital myasthenic syndrome; human ALG2 complements temperature sensitivity and dolichol-linked oligosaccharide biosynthesis defect of alg2-1 mutant, but mutant form from a patient with CDG-Ii fails to complement

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

379760..381271 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180930.1 → NP_011450.1 TPA: GDP-Man:Man(1)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011450.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU76, P43636

UniProtKB/TrEMBL

C7GW45, G2WE31, N1P9R0

Conserved Domains (1) summary

cd03805
Location:7 → 426

GT4_ALG2-like; alpha-1,3/1,6-mannosyltransferase ALG2 and similar proteins