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THI4 thiamine thiazole synthase [ Saccharomyces cerevisiae S288C ]

Gene ID: 853047, updated on 4-Jan-2025

Summary

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Official Symbol
THI4
Official Full Name
thiamine thiazole synthase
Primary source
SGD:S000003376
Locus tag
YGR144W
See related
AllianceGenome:SGD:S000003376; FungiDB:YGR144W; VEuPathDB:YGR144W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
ESP35; MOL1
Summary
Enables cysteine-dependent adenosine diphosphate thiazole synthase activity and ferrous iron binding activity. Involved in mitochondrial genome maintenance; thiamine biosynthetic process; and thiazole biosynthetic process. Located in cytosol. [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See THI4 in Genome Data Viewer
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (780399..781379)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene beta-glucan synthesis-associated protein SKN1 Neighboring gene tRNA-Gly Neighboring gene ribosome biosynthesis protein ENP2 Neighboring gene Ecl1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The Fusarium oxysporum sti35 gene functions in thiamine biosynthesis and oxidative stress response. Ruiz-Roldán C, et al. Fungal Genet Biol, 2008 Jan. PMID 17964830
  2. MOL1, a Saccharomyces cerevisiae gene that is highly expressed in early stationary phase during growth on molasses. Praekelt UM, et al. Yeast, 1992 Sep. PMID 1441749
  3. Regulation of THI4 (MOL1), a thiamine-biosynthetic gene of Saccharomyces cerevisiae. Praekelt UM, et al. Yeast, 1994 Apr. PMID 7941734
  4. Thiamin biosynthesis in eukaryotes: characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis. Chatterjee A, et al. J Am Chem Soc, 2006 Jun 7. PMID 16734458, Free PMC Article
  5. Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae. Jurgenson CT, et al. Biochemistry, 2006 Sep 19. PMID 16964967

See all (38) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. observations confirm NAD as the substrate for THI4 and elucidate the early steps of this unique biosynthesis of the thiazole moiety of thiamin in eukaryotes
    Title: Biosynthesis of thiamin thiazole in eukaryotes: conversion of NAD to an advanced intermediate.
  2. CyPBP37 is characterized as an abundant cytosolic protein homologous with yeast Thi4, and with a functional NAD-binding site.
    Title: Neurospora crassa CyPBP37: a cytosolic stress protein that is able to replace yeast Thi4p function in the synthesis of vitamin B1.
  3. structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae was determined to 1.8 A resolution
    Title: Structural insights into the function of the thiamin biosynthetic enzyme Thi4 from Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables cysteine-dependent adenosine diphosphate thiazole synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables cysteine-dependent adenosine diphosphate thiazole synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables ferrous iron binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables iron ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables iron ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables pentosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in mitochondrial genome maintenance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in thiamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in thiazole biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in thiazole biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in thiazole biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
thiamine thiazole synthase
NP_011660.1
  • Thiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    780399..781379
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181273.1 → NP_011660.1  TPA: thiamine thiazole synthase [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011660.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VUS4, P32318
    UniProtKB/TrEMBL
    A6ZUF6, B3LI86, B5VJ88, C7GSX8, G2WEM4, N1P438
    Conserved Domains (1) summary
    TIGR00292
    Location:45 → 325
    TIGR00292; thiazole biosynthesis enzyme
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32318.1

Gene LinkOut

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