ZUO1 zuotin [Saccharomyces cerevisiae S288C] - Gene

Summary

Go to the top of the page Help

Official Symbol: ZUO1
Official Full Name: zuotin
Primary source: SGD:S000003517
Locus tag: YGR285C
See related: AllianceGenome:SGD:S000003517; FungiDB:YGR285C; VEuPathDB:YGR285C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ribosome binding activity. Involved in gene expression; regulation of translational fidelity; and ribosomal subunit export from nucleus. Located in cytoplasm; nucleolus; and ribosome. Orthologous to human DNAJC2 (DnaJ heat shock protein family (Hsp40) member C2). [provided by Alliance of Genome Resources, Dec 2024]
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

See ZUO1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (1061852..1063153, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition.
Title: The ribosome-associated chaperone Zuo1 controls translation upon TORC1 inhibition.
Zuo1, a ribosome-associated J protein, is involved in glucose repression in Saccharomyces cerevisiae.
Title: Zuo1, a ribosome-associated J protein, is involved in glucose repression in Saccharomyces cerevisiae.
Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding.
Title: Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding.
Pathway of Hsp70 interactions at the ribosome.
Title: Pathway of Hsp70 interactions at the ribosome.
Zuo1 supports G4 structure formation and directs repair toward nucleotide excision repair.
Title: Zuo1 supports G4 structure formation and directs repair toward nucleotide excision repair.
Ssz1 is catalytically inert and cannot adopt the closed conformation, but the substrate binding domain beta is completed by Zuo1.
Title: Structural insights into a unique Hsp70-Hsp40 interaction in the eukaryotic ribosome-associated complex.
This study presents new structural and cross-linking data allowing more precise positioning of Saccharomyces cerevisiae ribosome-associated J protein-Hsp70 chaperone Zuo1 near the 60S polypeptide-exit site and suggesting interactions of Zuo1 with the ribosomal protein eL31 and 25S rRNA helix 24.
Title: Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunits.
The yeast ribosome-associated complex is composed of HSP40 (Zuotin) and HSP70 (Ssz1). RAC stabilizes the 80S ribosome in nonrotated, classical conformation.
Title: Structural basis for interaction of a cotranslational chaperone with the eukaryotic ribosome.
Unfolding of Zuo1's C-terminal helical bundle domain results in ribosome dissociation followed by activation of Pdr1 via a direct interaction.
Title: Unfolding of the C-terminal domain of the J-protein Zuo1 releases autoinhibition and activates Pdr1-dependent transcription.
study presents structural analyses of the ribosome-associated complex (RAC) consisting (Hsp70) Ssz1 and the Hsp40 Zuo1; a unique alpha-helical domain in Zuo1 mediates ribosome interaction of RAC near the ribosomal proteins L22e and L31e and ribosomal RNA helix H59
Title: Structural characterization of a eukaryotic chaperone--the ribosome-associated complex.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables Hsp70 protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables Hsp70 protein binding	IEA Inferred from Electronic Annotation more info
enables ribosome binding	IBA Inferred from Biological aspect of Ancestor more info
enables ribosome binding	IDA Inferred from Direct Assay more info	PubMed
enables ribosome binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 'de novo' cotranslational protein folding	IBA Inferred from Biological aspect of Ancestor more info
involved_in 'de novo' cotranslational protein folding	IEA Inferred from Electronic Annotation more info
involved_in 'de novo' cotranslational protein folding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of translational fidelity	IEA Inferred from Electronic Annotation more info
involved_in regulation of translational fidelity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosomal subunit export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translational frameshifting	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
located_in nucleolus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in ribosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: zuotin

NP_011801.1

Ribosome-associated co-chaperone; zuotin functions in ribosome biogenesis and as a chaperone for nascent polypeptide chains with Ssz1p and Ssb1/2p; subunit of the ribosome-associated complex (RAC) that stimulates Ssb1/2p ATPase activity to facilitate interaction with nascent polypeptide chains emerging from the ribosome exit tunnel; regulates translation upon inhibition of TORC1; contains a DnaJ domain; human gene DNAJC2 partially complements the yeast zuo1 null mutant

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

1061852..1063153 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181414.1 → NP_011801.1 TPA: zuotin [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011801.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VV62, P32527

UniProtKB/TrEMBL

B3LHW0, B5VJM9, C7GNF0, G2WF08, N1P9Z9

Conserved Domains (1) summary

COG5269
Location:50 → 433

ZUO1; Ribosome-associated chaperone zuotin [Translation, ribosomal structure and biogenesis / Posttranslational modification, protein turnover, chaperones]