POL31 DNA-directed DNA polymerase delta subunit POL31 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: POL31
Official Full Name: DNA-directed DNA polymerase delta subunit POL31
Primary source: SGD:S000003766
Locus tag: YJR006W
See related: AllianceGenome:SGD:S000003766; FungiDB:YJR006W; VEuPathDB:YJR006W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HUS2; HYS2; SDP5
Summary: Enables DNA-directed DNA polymerase activity. Involved in DNA replication, removal of RNA primer and RNA-templated DNA biosynthetic process. Located in cytosol and nucleus. Part of zeta DNA polymerase complex. Used to study cancer. Orthologous to human POLD2 (DNA polymerase delta 2, accessory subunit). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See POL31 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (449202..450665)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DNA polymerase delta: A single Pol31 polymorphism suppresses the strain background-specific lethality of Pol32 inactivation in Saccharomyces cerevisiae.
Title: DNA polymerase δ: A single Pol31 polymorphism suppresses the strain background-specific lethality of Pol32 inactivation in Saccharomyces cerevisiae.
Structure and mechanism of B-family DNA polymerase zeta specialized for translesion DNA synthesis.
Title: Structure and mechanism of B-family DNA polymerase ζ specialized for translesion DNA synthesis.
The structure reveals an unexpected arrangement in which the regulatory subunits (Pol31 and Pol32) lie next to the exonuclease domain of Pol3 but do not engage the DNA.
Title: Cryo-EM structure and dynamics of eukaryotic DNA polymerase δ holoenzyme.
Pol31 and Pol32 subunits of yeast DNA polymerase delta are also essential subunits of DNA polymerase zeta.
Title: Pol31 and Pol32 subunits of yeast DNA polymerase δ are also essential subunits of DNA polymerase ζ.
Loss of the last four amino acids at the C-terminal end of Pol3 impairs the interaction with Pol31.
Title: Stable interactions between DNA polymerase δ catalytic and structural subunits are essential for efficient DNA repair.
propose an orientation for the accessory Pol31-Pol32N subunits relative to the Pol3 catalytic core that best agrees with the experimental scattering profile
Title: Structural insights into yeast DNA polymerase delta by small angle X-ray scattering.
study reveals that Pol31 engages in crosstalk with the other Poldelta subunits, as well as with Mgs1, Rad18 and PCNA; some of the Pol31 regions that are important for such functional cooperations are identified
Title: Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
contributes_to DNA-directed DNA polymerase activity	TAS Traceable Author Statement more info	PubMed
contributes_to single-stranded DNA 3'-5' DNA exonuclease activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication, removal of RNA primer	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA strand elongation involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in RNA-templated DNA biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in base-excision repair	TAS Traceable Author Statement more info	PubMed
involved_in lagging strand elongation	TAS Traceable Author Statement more info	PubMed
involved_in leading strand elongation	TAS Traceable Author Statement more info	PubMed
involved_in mismatch repair	TAS Traceable Author Statement more info	PubMed
involved_in nucleotide-excision repair	TAS Traceable Author Statement more info	PubMed
involved_in postreplication repair	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of delta DNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of delta DNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of delta DNA polymerase complex	TAS Traceable Author Statement more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of zeta DNA polymerase complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA-directed DNA polymerase delta subunit POL31

NP_012539.1

EC 2.7.7.7

Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair; forms a complex with Rev3p, Rev7p and Pol32p; relocalizes to the cytosol in response to hypoxia

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

449202..450665

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181663.1 → NP_012539.1 TPA: DNA-directed DNA polymerase delta subunit POL31 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012539.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWI1, P46957

UniProtKB/TrEMBL

D3UF73, N1P1G3

Conserved Domains (1) summary

COG1311
Location:5 → 487

HYS2; Archaeal DNA polymerase II, small subunit/DNA polymerase delta, subunit B [Replication, recombination and repair]