RAD26 DNA-dependent ATPase RAD26 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD26
Official Full Name: DNA-dependent ATPase RAD26
Primary source: SGD:S000003796
Locus tag: YJR035W
See related: AllianceGenome:SGD:S000003796; FungiDB:YJR035W; VEuPathDB:YJR035W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ATP-dependent activity, acting on DNA. Involved in nucleotide-excision repair and regulation of transcription by RNA polymerase II. Acts upstream of or within transcription-coupled nucleotide-excision repair. Located in cytoplasm and nucleus. Used to study Cockayne syndrome B. Human ortholog(s) of this gene implicated in several diseases, including Cockayne syndrome (multiple); De Sanctis-Cacchione syndrome; UV-sensitive syndrome; gastrointestinal system cancer (multiple); and respiratory system cancer (multiple). Orthologous to human ERCC6 (ERCC excision repair 6, chromatin remodeling factor). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See RAD26 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (497355..500612)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Elf1 promotes Rad26's interaction with lesion-arrested Pol II for transcription-coupled repair.
Title: Elf1 promotes Rad26's interaction with lesion-arrested Pol II for transcription-coupled repair.
Strand-specific effect of Rad26 and TFIIS in rescuing transcriptional arrest by CAG trinucleotide repeat slip-outs.
Title: Strand-specific effect of Rad26 and TFIIS in rescuing transcriptional arrest by CAG trinucleotide repeat slip-outs.
Genome-wide role of Rad26 in promoting transcription-coupled nucleotide excision repair in yeast chromatin.
Title: Genome-wide role of Rad26 in promoting transcription-coupled nucleotide excision repair in yeast chromatin.
Transcriptional nucleotide misincorporation is not implicated in TC-NER, and moderate eviction of Spt5 and promotion of error-free transcriptional bypass of DNA lesions by Rad26 facilitates TC-NER.
Title: Evidence that Moderate Eviction of Spt5 and Promotion of Error-Free Transcriptional Bypass by Rad26 Facilitates Transcription Coupled Nucleotide Excision Repair.
structure of the S. cerevisiae Pol II-Rad26 complex solved by cryo-electron microscopy; structure reveals that Rad26 binds to the DNA upstream of Pol II, where it markedly alters its path; structural and functional data suggest that the conserved Swi2/Snf2-family core ATPase domain promotes the forward movement of Pol II, and elucidate key roles for Rad26 in both TCR and transcription elongation
Title: Structural basis for the initiation of eukaryotic transcription-coupled DNA repair.
Rad26 may facilitate transcription coupled nucleotide excision repair (TC-NER) indirectly, by antagonizing the action of TC-NER repressors that normally promote transcription elongation.
Title: Transcription coupled nucleotide excision repair in the yeast Saccharomyces cerevisiae: The ambiguous role of Rad26.
Data indicate that RAD26 deletion does not reduce expression of nucleotide excision repair (NER) factors but affects RPB2 transcription during NER.
Title: Rad26, the transcription-coupled repair factor in yeast, is required for removal of stalled RNA polymerase-II following UV irradiation.
Rad26p promotes the eviction of histone H2A-H2B dimer and hence chromatin disassembly.
Title: Rad26p, a transcription-coupled repair factor, promotes the eviction and prevents the reassociation of histone H2A-H2B dimer during transcriptional elongation in vivo.
Rad26p does not recognize DNA lesions in the absence of active transcription.
Title: Rad26p, a transcription-coupled repair factor, is recruited to the site of DNA lesion in an elongating RNA polymerase II-dependent manner in vivo.
Spt5 CTR suppresses Rad26-independent TCR by serving as a platform for assembly of a multiple protein suppressor complex that is associated with Pol II.
Title: The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on DNA	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent chromatin remodeler activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables forked DNA-dependent helicase activity	IEA Inferred from Electronic Annotation more info
enables four-way junction helicase activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables single-stranded 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in nucleotide-excision repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription-coupled nucleotide-excision repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription-coupled nucleotide-excision repair	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within transcription-coupled nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription-coupled nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-dependent ATPase RAD26

NP_012569.1

EC 3.6.4.12

Protein involved in transcription-coupled nucleotide excision repair; repairs UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

497355..500612

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181693.1 → NP_012569.1 TPA: DNA-dependent ATPase RAD26 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012569.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWK8, P40352

UniProtKB/TrEMBL

G2WH41, N1P3E3

Conserved Domains (2) summary

COG0553
Location:2 → 824

HepA; Superfamily II DNA or RNA helicase, SNF2 family [Transcription, Replication, recombination and repair]

cd18000
Location:297 → 517

DEXHc_ERCC6; DEXH-box helicase domain of ERCC6