SNU114 U5 snRNP GTPase SNU114 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SNU114
Official Full Name: U5 snRNP GTPase SNU114
Primary source: SGD:S000001656
Locus tag: YKL173W
See related: AllianceGenome:SGD:S000001656; FungiDB:YKL173W; VEuPathDB:YKL173W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: GIN10
Summary: Enables GTPase activity and U5 snRNA binding activity. Involved in generation of catalytic spliceosome for first transesterification step; spliceosomal tri-snRNP complex assembly; and spliceosome conformational change to release U4 (or U4atac) and U1 (or U11). Located in nucleus. Part of U4/U6 x U5 tri-snRNP complex and U5 snRNP. Used to study mandibulofacial dysostosis, Guion-Almeida type. Human ortholog(s) of this gene implicated in esophageal atresia; hepatitis B; hepatocellular carcinoma; mandibulofacial dysostosis, Guion-Almeida type; and microcephaly. Orthologous to human EFTUD2 (elongation factor Tu GTP binding domain containing 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SNU114 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (122517..125543)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results suggest that the Snu114-GTP-Prp8 module serves as a relay station during spliceosome activation and disassembly, but that GTPase activity may be dispensable for splicing.
Title: A Snu114-GTP-Prp8 module forms a relay station for efficient splicing in yeast.
We propose that the U5 snRNA IL1 is a platform for protein binding and is required for , Brr2 and Snu114 association with the U5 snRNA to form the U5 snRNP.
Title: The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.
Data suggest that the RNA base pairing state is directly or indirectly sensed by the Snu114p G domain.
Title: Analysis of synthetic lethality reveals genetic interactions between the GTPase Snu114p and snRNAs in the catalytic core of the Saccharomyces cerevisiae spliceosome.
Results report the structure of the Saccharomyces cerevisiae tri-snRNP, and show that U4/U6 snRNP forms a domain termed the arm, while a separate head domain adjacent to the arm harbors Brr2, and Prp8 and the GTPase Snu114 are located centrally.
Title: Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy.
Prp8 interaction site with Snu114 protein
Title: Prp8p dissection reveals domain structure and protein interaction sites.
Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.
Title: Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.
GTP hydrolysis results in a rearrangement between Prp8 and the C terminus of Snu114 that leads to release of U1 and U4, thus activating the spliceosome for catalysis.
Title: Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation.
Snu114p serves as a signal-dependent switch that transduces signals to Brr2p to control spliceosome dynamics.
Title: The EF-G-like GTPase Snu114p regulates spliceosome dynamics mediated by Brr2p, a DExD/H box ATPase.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables U5 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U5 snRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in generation of catalytic spliceosome for first transesterification step	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA splicing, via spliceosome	IBA Inferred from Biological aspect of Ancestor more info
involved_in mRNA splicing, via spliceosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spliceosomal tri-snRNP complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of U2-type catalytic step 2 spliceosome	IBA Inferred from Biological aspect of Ancestor more info
part_of U4/U6 x U5 tri-snRNP complex	IBA Inferred from Biological aspect of Ancestor more info
part_of U4/U6 x U5 tri-snRNP complex	IDA Inferred from Direct Assay more info	PubMed
part_of U5 snRNP	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: U5 snRNP GTPase SNU114

NP_012748.1

GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

122517..125543

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179739.1 → NP_012748.1 TPA: U5 snRNP GTPase SNU114 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012748.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VX27, P36048

UniProtKB/TrEMBL

A6ZZF2, G2WHL9, N1P2W5

Conserved Domains (1) summary

COG0480
Location:130 → 975

FusA; Translation elongation factor EF-G, a GTPase [Translation, ribosomal structure and biogenesis]