APN1 DNA-(apurinic or apyrimidinic site) lyase APN1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: APN1
Official Full Name: DNA-(apurinic or apyrimidinic site) lyase APN1
Primary source: SGD:S000001597
Locus tag: YKL114C
See related: AllianceGenome:SGD:S000001597; FungiDB:YKL114C; VEuPathDB:YKL114C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 3'-tyrosyl-DNA phosphodiesterase activity; DNA-(apurinic or apyrimidinic site) endonuclease activity; and double-stranded DNA 3'-5' DNA exonuclease activity. Involved in base-excision repair. Located in mitochondrion and nucleus. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See APN1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (223352..224455, complement)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The activity of yeast Apn2 AP endonuclease at uracil-derived AP sites is dependent on the major carbon source.
Title: The activity of yeast Apn2 AP endonuclease at uracil-derived AP sites is dependent on the major carbon source.
His-83 of Apn1 properly coordinates the active site Zn2+ ion playing a crucial role in catalytic incision stage during DNA repair.
Title: The role of His-83 of yeast apurinic/apyrimidinic endonuclease Apn1 in catalytic incision of abasic sites in DNA.
Frataxin (YFH1) deficiency in yeast cells leads to increased DNA base oxidation and requirement of Apn1 for DNA damage repair.
Title: Apn1 AP-endonuclease is essential for the repair of oxidatively damaged DNA bases in yeast frataxin-deficient cells.
steady state levels of Apn1 V156E were substantially decreased compared to wild V156 residue plays a critical role in Apn1 stability, but is not involved in catalytic activity.
Title: Saccharomyces cerevisiae Apn1 mutation affecting stable protein expression mimics catalytic activity impairment: implications for assessing DNA repair capacity in humans.
Direct physical evidence that Apn1 not only repair cellular base damage but also prevent break accumulation that can result from abasic sites being channeled into other base excision repair pathway(s).
Title: Apn1 and Apn2 endonucleases prevent accumulation of repair-associated DNA breaks in budding yeast as revealed by direct chromosomal analysis.
Novel Apn1 substrates alpha-2'-deoxyadenosine and alpha-thymidine play an important role in counteracting oxidative DNA base damage.
Title: Alpha-anomeric deoxynucleotides, anoxic products of ionizing radiation, are substrates for the endonuclease IV-type AP endonucleases.
Apn1 is involved in an S. cerevisiae 8-oxoguanine-DNA glycosylase (Ogg1)-independent repair pathway for 8-oxo-7,8-dihydrodeoxyguanosine residues.
Title: The 3'->5' exonuclease of Apn1 provides an alternative pathway to repair 7,8-dihydro-8-oxodeoxyguanosine in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-tyrosyl-DNA phosphodiesterase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-(apurinic or apyrimidinic site) endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables double-stranded DNA 3'-5' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphoric diester hydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoric diester hydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in base-excision repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in base-excision repair	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-(apurinic or apyrimidinic site) lyase APN1

NP_012808.1

Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

223352..224455 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179680.1 → NP_012808.1 TPA: DNA-(apurinic or apyrimidinic site) lyase APN1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012808.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXH4, P22936

UniProtKB/TrEMBL

A6ZZK3, B5VM45, G2WHS0, N1P170

Conserved Domains (1) summary

TIGR00587
Location:15 → 290

nfo; apurinic endonuclease (APN1)