RPB2 DNA-directed RNA polymerase II core subunit RPB2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPB2
Official Full Name: DNA-directed RNA polymerase II core subunit RPB2
Primary source: SGD:S000005677
Locus tag: YOR151C
See related: AllianceGenome:SGD:S000005677; FungiDB:YOR151C; VEuPathDB:YOR151C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RPB150; RPO22; SIT2; SOH2
Summary: Enables DNA binding activity. Contributes to RNA polymerase II activity and RNA-dependent RNA polymerase activity. Involved in transcription by RNA polymerase II. Located in cytoplasmic stress granule; mitochondrion; and nucleus. Part of RNA polymerase II, core complex. Orthologous to human POLR2B (RNA polymerase II subunit B). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See RPB2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (612997..616671, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rtr1 is required for Rpb1-Rpb2 assembly of RNAPII and prevents their cytoplasmic clump formation.
Title: Rtr1 is required for Rpb1-Rpb2 assembly of RNAPII and prevents their cytoplasmic clump formation.
Depletion of RNA polymerase II induces the long form of RPB2 mRNA.
Title: RNA polymerase II depletion promotes transcription of alternative mRNA species.
Rpb1/2 dynamics help govern the decision between sense and divergent antisense transcription.
Title: RNA Pol II Dynamics Modulate Co-transcriptional Chromatin Modification, CTD Phosphorylation, and Transcriptional Direction.
Data indicate that RAD26 deletion does not reduce expression of nucleotide excision repair (NER) factors but affects RPB2 transcription during NER.
Title: Rad26, the transcription-coupled repair factor in yeast, is required for removal of stalled RNA polymerase-II following UV irradiation.
the 3'UTR of RPB2 is sufficient for this UV-induced regulation of alternative polyadenylation.
Title: UV damage regulates alternative polyadenylation of the RPB2 gene in yeast.
study examined the properties of the corresponding RNAPII (Rpb2-832GYNQED837) and RNAPIII (Rpc128-764GYDIED769) loops by a local mutagenesis of their invariant carboxyl acids
Title: Crucial role of a dicarboxylic motif in the catalytic center of yeast RNA polymerases.
Study points strongly toward a minor role of TFIIH kinase in Pol II transcription, and a more significant role in mRNA capping in budding yeast.
Title: Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5'-3' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed 5'-3' RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase I activity	IEA Inferred from Electronic Annotation more info
contributes_to RNA polymerase II activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to RNA polymerase II activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase III activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase IV activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase V activity	IEA Inferred from Electronic Annotation more info
contributes_to RNA-dependent RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	HDA more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables mitochondrial single subunit type RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info
enables plastid single subunit type RNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables ribonucleoside binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in mitochondrial transcription	IEA Inferred from Electronic Annotation more info
involved_in plastid transcription	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase I	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase III	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase IV	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase V	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase II, core complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II, core complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-directed RNA polymerase II core subunit RPB2

NP_014794.3

EC 2.7.7.6

RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

612997..616671 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183570.3 → NP_014794.3 TPA: DNA-directed RNA polymerase II core subunit RPB2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014794.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2K8, P08518, Q12738, Q7Z9Y0

UniProtKB/TrEMBL

A6ZNZ5, C7GN73, C8ZGQ9, G2WN23, N1P384

Conserved Domains (1) summary

PRK08565
Location:31 → 1216

PRK08565; DNA-directed RNA polymerase subunit B; Provisional