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SCEI intron-encoded endonuclease I-SceI [ Saccharomyces cerevisiae S288C ]

Gene ID: 854590, updated on 4-Jan-2025

Summary

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Official Symbol
SCEI
Official Full Name
intron-encoded endonuclease I-SceI
Primary source
SGD:S000007279
Locus tag
Q0160
See related
AllianceGenome:SGD:S000007279; FungiDB:Q0160; VEuPathDB:Q0160
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Saccharomyces cerevisiae S288C (strain: S288C, substrain: FY1679)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables endonuclease activity. Predicted to be involved in intron homing. Predicted to be located in mitochondrion. [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See SCEI in Genome Data Viewer
Sequence:
Chromosome: MT; NC_001224.1 (61022..61729)

Chromosome MT - NC_001224.1Genomic Context describing neighboring genes Neighboring gene tRNA-Ser Neighboring gene mitochondrial 37S ribosomal protein VAR1 Neighboring gene 21S ribosomal RNA Neighboring gene tRNA-Thr Neighboring gene tRNA-Cys

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001224.1

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Transposition of an intron in yeast mitochondria requires a protein encoded by that intron. Macreadie IG, et al. Cell, 1985 Jun. PMID 3886164
  2. Nested chromosomal fragmentation in yeast using the meganuclease I-Sce I: a new method for physical mapping of eukaryotic genomes. Thierry A, et al. Nucleic Acids Res, 1992 Nov 11. PMID 1333585, Free PMC Article
  3. Amino acid residues in both the protein splicing and endonuclease domains of the PI-SceI intein mediate DNA binding. He Z, et al. J Biol Chem, 1998 Feb 20. PMID 9468518
  4. Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron. Monteilhet C, et al. Nucleic Acids Res, 1990 Mar 25. PMID 2183191, Free PMC Article
  5. Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells. Rouet P, et al. Proc Natl Acad Sci U S A, 1994 Jun 21. PMID 8016116, Free PMC Article

See all (47) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A metal binding site is present in the active site of I-SceI that cleaves the top strand DNA.
    Title: Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway.
  2. findings suggest that the HSP70-subunit shields Endo.SceI from heat inactivation through ATP/ADP binding
    Title: Roles of the HSP70-subunit in a eukaryotic multi-site-specific endonuclease, Endo.SceI: autophosphorylation and heat stability.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in intron homing IEA
Inferred from Electronic Annotation
more info
  
involved_in intron homing TAS
Traceable Author Statement
more info
 PubMed 
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  
located_in mitochondrion TAS
Traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
intron-encoded endonuclease I-SceI
NP_009324.1
  • I-SceI DNA endonuclease; encoded by the mitochondrial group I intron of the 21S_rRNA gene; mediates gene conversion that propagates the intron into intron-less copies of the 21S_rRNA gene

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001224.1 Reference assembly

    Range
    61022..61729
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_009324.1 intron-encoded endonuclease I-SceI (mitochondrion) [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009324.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    A0A0A7NYJ1, P03882
    UniProtKB/TrEMBL
    A0A0H3WJ15, A7LCP1, E9P9U6
    Conserved Domains (1) summary
    pfam03161
    Location:34 → 208
    LAGLIDADG_2; LAGLIDADG DNA endonuclease family
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P03882.1

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