MGA2 Mga2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MGA2
Official Full Name: Mga2p
Primary source: SGD:S000001472
Locus tag: YIR033W
See related: AllianceGenome:SGD:S000001472; FungiDB:YIR033W; VEuPathDB:YIR033W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Involved in several processes, including cellular response to cobalt ion; cellular response to iron ion starvation; and regulation of biosynthetic process. Located in endoplasmic reticulum membrane and nucleus. [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See MGA2 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (416124..419465)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

By combining molecular dynamics simulations with experiments, we uncover a remarkable sensitivity of the transcriptional regulator Mga2 to the abundance, position, and configuration of double bonds in lipid acyl chains, and provide insights into the molecular rules of membrane adaptation.
Title: Regulation of lipid saturation without sensing membrane fluidity.
Lipid environment controls the proteolytic activation of Mga2 by stabilizing alternative rotational orientations of the TMH region. Mga2 can act as a lipid-packing sensor in the ER membrane to control the production of unsaturated fatty acids.
Title: A Eukaryotic Sensor for Membrane Lipid Saturation.
the spt23Delta mutant strain shows similar ERG1 expression to wild-type cells, while the mga2Delta/spt23Delta strain shows reduced ERG1 expression, comparable to the mga2Delta, suggesting that the role of regulation of ERG1 transcription is unique to Mga2p.
Title: A role for MGA2, but not SPT23, in activation of transcription of ERG1 in Saccharomyces cerevisiae.
WW domains 2 and 3 of Rsp5p play overlapping roles in binding to the WW domain-binding site site on Spt23p and Mga2p
Title: WW domains 2 and 3 of Rsp5p play overlapping roles in binding to the LPKY motif of Spt23p and Mga2p.
identified, using mass spectrometry analysis of in vitro ubiquitinated Mga2p120-Mga2p90 complex, that lysine residues 983 and 985 contained within the carboxy-terminal domain of Mga2p120 are Rsp5p-directed Ub-conjugation sites
Title: Identification of lysines within membrane-anchored Mga2p120 that are targets of Rsp5p ubiquitination and mediate mobilization of tethered Mga2p90.
Rsp5p, Spt23p and Mga2p may participate in the control of the homeostasis of lipids and lipid particles.
Title: Rsp5p ubiquitin ligase and the transcriptional activators Spt23p and Mga2p are involved in co-regulation of biosynthesis of end products of the mevalonate pathway and triacylglycerol in yeast Saccharomyces cerevisiae.
a PXY motif located within the carboxyl terminus of Spt23p and Mga2p mediates a physical and functional interaction with ubiquitin ligase Rsp5p
Title: A single PXY motif located within the carboxyl terminus of Spt23p and Mga2p mediates a physical and functional interaction with ubiquitin ligase Rsp5p.
proteasomal processing of the Mga2 is initiated by an internal cleavage, followed by bidirectional proteolysis of the polypeptides
Title: Proteasome-mediated protein processing by bidirectional degradation initiated from an internal site.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in cellular response to cobalt ion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to iron ion starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of unsaturated fatty acid biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Mga2p

NP_012299.1

ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

416124..419465

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179555.1 → NP_012299.1 TPA: Mga2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012299.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVW4, P40578

UniProtKB/TrEMBL

A6ZVV3, G2WFW1

Conserved Domains (4) summary

PLN03192
Location:622 → 867

PLN03192; Voltage-dependent potassium channel; Provisional

cd00102
Location:530 → 626

IPT; Immunoglobulin-like fold, Plexins, Transcription factors (IPT). IPTs are also known as Transcription factor ImmunoGlobin (TIG) domains. They are present in intracellular transcription factors, cell surface receptors (such as plexins and scatter factor ...

sd00045
Location:719 → 750

ANK; ANK repeat [structural motif]

pfam12796
Location:707 → 782

Ank_2; Ankyrin repeats (3 copies)