FKH2 forkhead family transcription factor FKH2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: FKH2
Official Full Name: forkhead family transcription factor FKH2
Primary source: SGD:S000005012
Locus tag: YNL068C
See related: AllianceGenome:SGD:S000005012; FungiDB:YNL068C; VEuPathDB:YNL068C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables several functions, including DNA replication origin binding activity; RNA polymerase II-specific DNA-binding transcription factor binding activity; and transcription coactivator activity. Involved in several processes, including mitotic cell cycle phase transition; negative regulation of pseudohyphal growth; and regulation of macromolecule metabolic process. Located in cytosol and nucleus. Human ortholog(s) of this gene implicated in primary ciliary dyskinesia 43. Orthologous to human FOXJ1 (forkhead box J1). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See FKH2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (495701..498289, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

New roles of DNA-binding and forkhead-associated domains of Fkh1 and Fkh2 in cellular functions.
Title: New roles of DNA-binding and forkhead-associated domains of Fkh1 and Fkh2 in cellular functions.
Fkh1 and Fkh2 transcription factors are hubs that integrate multi-scale networks in budding yeast.
Title: ChIP-exo analysis highlights Fkh1 and Fkh2 transcription factors as hubs that integrate multi-scale networks in budding yeast.
Fkh1 and Fkh2 dimerization controls DNA replication timing and spatial organization of chromosomes.
Title: Conserved forkhead dimerization motif controls DNA replication timing and spatial organization of chromosomes in S. cerevisiae.
It was found that ABF1, FKH2, and REB1 overexpression decreased the growth of strains in a prion-dependent manner and, thus, might influence [PSI;(+)] prion toxicity.
Title: [Identification of new genes that affect [PSI^(+)] prion toxicity in Saccharomyces cerevisiae yeast].
This study establishes the FKH1 and FKH2 role as evolutionarily conserved regulators of lifespan in yeast and identifies the APC as a novel component of this mechanism
Title: The yeast forkhead transcription factors fkh1 and fkh2 regulate lifespan and stress response together with the anaphase-promoting complex.
Study shows that Fkh1 and Fkh2 regulate the initiation timing of most of the earliest origins in the yeast genome through a novel mechanism involving origin clustering in G1 phase.
Title: Forkhead transcription factors establish origin timing and long-range clustering in S. cerevisiae.
The Mcm1-Fkh2 complex, first shown to transactivate genes within the CLB2 cluster that drive G(2)/M progression, also associated directly at the PHO5 promoter in a cell cycle-dependent manner.
Title: Coupling phosphate homeostasis to cell cycle-specific transcription: mitotic activation of Saccharomyces cerevisiae PHO5 by Mcm1 and Forkhead proteins.
Disruption of fkh2-associated cell cycle transcription by oxidative stress
Title: Disruption of yeast forkhead-associated cell cycle transcription by oxidative stress.
Fkh2p is a target of cyclin-dependent protein kinases, and phosphorylation of Fkh2p promotes interactions between Fkh2p and the coactivator Ndd1p
Title: Regulation of cell cycle-specific gene expression through cyclin-dependent kinase-mediated phosphorylation of the forkhead transcription factor Fkh2p.
Chromatin-mediated repression by Isw1 and Isw2 may serve to limit activation of CLB2 expression by the Clb2/Cdk1 kinase during G(2)/M and to fully repress expression during G(1).
Title: The Isw2 chromatin-remodeling ATPase cooperates with the Fkh2 transcription factor to repress transcription of the B-type cyclin gene CLB2.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IGI Inferred from Genetic Interaction more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	HDA more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription coregulator activity	IDA Inferred from Direct Assay more info	PubMed
enables transcription coregulator activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in G1/S transition of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in G2/M transition of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin remodeling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in chromatin remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of pseudohyphal growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of pseudohyphal growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of silent mating-type cassette heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of silent mating-type cassette heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: forkhead family transcription factor FKH2

NP_014331.3

Forkhead family transcription factor; rate-limiting activator of replication origins; evolutionarily conserved regulator of lifespan; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; positively regulates transcriptional elongation; facilitates clustering, activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; major role in expression of G2/M phase genes; relocalizes to cytosol under hypoxia

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

495701..498289 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182906.3 → NP_014331.3 TPA: forkhead family transcription factor FKH2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014331.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1B1, P41813

UniProtKB/TrEMBL

B5VQX6, C8ZGG2

Conserved Domains (1) summary

COG5025
Location:1 → 610

COG5025; Transcription factor of the Forkhead/HNF3 family [Transcription]