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KAR9 Kar9p [ Saccharomyces cerevisiae S288C ]

Gene ID: 855859, updated on 9-Dec-2024

Summary

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Official Symbol
KAR9
Official Full Name
Kar9p
Primary source
SGD:S000006190
Locus tag
YPL269W
See related
AllianceGenome:SGD:S000006190; FungiDB:YPL269W; VEuPathDB:YPL269W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Involved in establishment of spindle localization; mitotic spindle orientation checkpoint signaling; and nuclear migration along microtubule. Located in several cellular components, including kinetochore; mating projection tip; and microtubule cytoskeleton. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See KAR9 in Genome Data Viewer
Location:
chromosome: XVI
Exon count:
1
Sequence:
Chromosome: XVI; NC_001148.4 (33013..34947)

Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene F1F0 ATP synthase subunit epsilon Neighboring gene ATP-binding cassette permease MDL2 Neighboring gene phosphatidylinositol phospholipase C Neighboring gene Acm1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends. Kumar A, et al. Structure, 2017 Jun 6. PMID 28552577
  2. Purification, crystallization and preliminary crystallographic analysis of Kar9p from Saccharomyces cerevisiae. Hüls D, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2012 Oct 1. PMID 23027760, Free PMC Article
  3. Temporal control of contact site formation reveals a relationship between mitochondrial division and Num1-mediated mitochondrial tethering. Harper CS, et al. Mol Biol Cell, 2023 Oct 1. PMID 37585290, Free PMC Article
  4. Astral microtubule pivoting promotes their search for cortical anchor sites during mitosis in budding yeast. Baumgärtner S, et al. PLoS One, 2014. PMID 24721997, Free PMC Article
  5. Molecular linkage underlying microtubule orientation toward cortical sites in yeast. Korinek WS, et al. Science, 2000 Mar 24. PMID 10731146

See all (131) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Activation of the DNA damage checkpoint perturbs asymmetric localization of Kar9 to spindle pole bodies in Saccharomyces cerevisiae.
    Title: Activation of the DNA damage checkpoint perturbs asymmetric localization of Kar9 to spindle pole bodies in Saccharomyces cerevisiae.
  2. Multivalency ensures persistence of a +TIP body at specialized microtubule ends.
    Title: Multivalency ensures persistence of a +TIP body at specialized microtubule ends.
  3. KAR9 has microtubule end-binding motif LxxPTPh (where x and h represent any and hydrophobic amino acids, respectively).
    Title: Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends.
  4. One crystal of Kar9p showed diffraction to 3 A resolution
    Title: Purification, crystallization and preliminary crystallographic analysis of Kar9p from Saccharomyces cerevisiae.
  5. Kar9 is ubiquitylated and degraded by the proteasome after phosphorylation by Cdk1.
    Title: Ubiquitylation regulates interactions of astral microtubules with the cleavage apparatus.
  6. sumoylation regulates spindle positioning by restricting Kar9p to one spindle pole body.
    Title: The spindle positioning protein Kar9p interacts with the sumoylation machinery in Saccharomyces cerevisiae.
  7. Three independent mechanisms contribute to Kar9 asymmetry: Cdk1-dependent phosphorylation, sumoylation, and spindle-assembly checkpoint signaling.
    Title: Regulation of mitotic spindle asymmetry by SUMO and the spindle-assembly checkpoint in yeast.
  8. Kar9p-mediated MT delivery along actin cables does not involve Bud6p at the cell cortex
    Title: Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae.
  9. Overproduction of Bim1, but not Kar9, restores Kar9-dependent spindle positioning in the tub4-delta dsyl mutant, reduces cortical dwelling, and promotes Bim1-Kar9 interactions.
    Title: Gamma-tubulin is required for proper recruitment and assembly of Kar9-Bim1 complexes in budding yeast.
  10. The asymmetric localization of Kar9p to one spindle pole body and attached cytoplasmic microtubules is dependent on its interactions with microtubule-associated proteins and is regulated by the yeast Cdc28p
    Title: The cyclin-dependent kinase Cdc28p regulates multiple aspects of Kar9p function in yeast.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables molecular_function ND
No biological Data available
more info
  
Process Evidence Code Pubs
involved_in establishment of spindle localization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in establishment of spindle localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in karyogamy IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic spindle orientation checkpoint signaling IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitotic spindle orientation checkpoint signaling IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in nuclear migration along microtubule IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nuclear migration along microtubule IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cell cortex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell cortex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasmic microtubule IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in mating projection tip IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mating projection tip IDA
Inferred from Direct Assay
more info
 PubMed 
located_in microtubule IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
is_active_in spindle pole body IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in spindle pole body IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
Kar9p
NP_015054.1
  • Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001148.4 Reference assembly

    Range
    33013..34947
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184083.1 → NP_015054.1  TPA: Kar9p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_015054.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W3A1, P32526, Q08982
    UniProtKB/TrEMBL
    B3LKG4, B5VSR9, C7GSG9, C8ZIB0, N1NVN2
    Conserved Domains (1) summary
    pfam08580
    Location:84 → 406
    KAR9; Yeast cortical protein KAR9
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32526.2

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