SCC4 cohesin-loading factor complex subunit SCC4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SCC4
Official Full Name: cohesin-loading factor complex subunit SCC4
Primary source: SGD:S000000949
Locus tag: YER147C
See related: AllianceGenome:SGD:S000000949; FungiDB:YER147C; VEuPathDB:YER147C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables kinetochore binding activity. Involved in chromosome organization; double-strand break repair; and establishment of protein localization to chromatin. Located in chromatin. Part of SMC loading complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SCC4 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (462968..464842, complement)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Recruitment of Scc2/4 to double-strand breaks depends on gammaH2A and DNA end resection.
Title: Recruitment of Scc2/4 to double-strand breaks depends on γH2A and DNA end resection.
Folding of cohesin's coiled coil is important for Scc2/4-induced association with chromosomes.
Title: Folding of cohesin's coiled coil is important for Scc2/4-induced association with chromosomes.
A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules.
Title: A conserved ATP- and Scc2/4-dependent activity for cohesin in tethering DNA molecules.
Here we reveal a dual role of RSC in cohesin loading. RSC acts as a chromatin receptor that recruits Scc2-Scc4 by a direct protein interaction independent of chromatin remodeling. In addition, chromatin remodeling is required to generate a nucleosome-free region that is the substrate for cohesin loading.
Title: A Role for Chromatin Remodeling in Cohesin Loading onto Chromosomes.
The role of Scc4 in cohesin loading
Title: Identification of Functional Domains in the Cohesin Loader Subunit Scc4 by a Random Insertion/Dominant Negative Screen.
These findings reveal the role of Scc4 in determining the localization of cohesin loading and establish a molecular basis for Scc2/4 recruitment to centromeres.
Title: Structural evidence for Scc4-dependent localization of cohesin loading.
Data indicate that Scc2-Scc4 binding sites were strikingly enriched at transcriptional start sites (TSSs).
Title: The Scc2-Scc4 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions.
Scc2/Scc4 resides at previously mapped cohesin-associated regions (CARs) in pericentromeric and arm regions, and that Scc2/Scc4-cohesin colocalization persists after the initial deposition of cohesins in G1/S phase
Title: The Scc2/Scc4 cohesin loader determines the distribution of cohesin on budding yeast chromosomes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables kinetochore binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of protein localization to chromatin	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic chromosome condensation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid cohesion	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rDNA chromatin condensation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of SMC loading complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cohesin-loading factor complex subunit SCC4

NP_011074.3

Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

462968..464842 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179037.3 → NP_011074.3 TPA: cohesin-loading factor complex subunit SCC4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011074.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM54, P40090

UniProtKB/TrEMBL

A6ZRA4, B3LRR0, C7GQ49, C8Z7G8, N1P768

Conserved Domains (1) summary

pfam10345
Location:13 → 624

Cohesin_load; Cohesin loading factor