DNF1 aminophospholipid-translocating P4-type ATPase DNF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DNF1
Official Full Name: aminophospholipid-translocating P4-type ATPase DNF1
Primary source: SGD:S000000968
Locus tag: YER166W
See related: AllianceGenome:SGD:S000000968; FungiDB:YER166W; VEuPathDB:YER166W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables glycosylceramide flippase activity; phosphatidylcholine flippase activity; and phosphatidylethanolamine flippase activity. Involved in several processes, including endocytosis; intracellular protein transport; and lipid translocation. Located in mating projection tip membrane. Part of phospholipid-translocating ATPase complex. Is active in plasma membrane. Human ortholog(s) of this gene implicated in benign recurrent intrahepatic cholestasis 1; intrahepatic cholestasis; intrahepatic cholestasis of pregnancy 1; and progressive familial intrahepatic cholestasis 1. Orthologous to several human genes including ATP10A (ATPase phospholipid transporting 10A (putative)) and ATP10D (ATPase phospholipid transporting 10D (putative)). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See DNF1 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (512744..517459)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lipid flippases in polarized growth.
Title: Lipid flippases in polarized growth.
Transport mechanism of P4 ATPase phosphatidylcholine flippases.
Title: Transport mechanism of P4 ATPase phosphatidylcholine flippases.
An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast.
Title: An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast.
A mutagenesis screen identified several gain-of-function mutations in the P4-ATPase Dnf1 that facilitate the transport of a novel lipid substrate, sphingomyelin.
Title: Directed evolution of a sphingomyelin flippase reveals mechanism of substrate backbone discrimination by a P4-ATPase.
Dnf1 poorly recognizes diacylated phospholipid and define key substitutions enabling recognition of endogenous PS.
Title: Type IV P-type ATPases distinguish mono- versus diacyl phosphatidylserine using a cytofacial exit gate in the membrane domain.
Studie identified mutations that cause a gain of phosphatidylserine (PS) flip by type IV P-type ATPase Dnf1 or a loss of PS flip by type IV P-type ATPase Drs2.
Title: Two-gate mechanism for phospholipid selection and transport by type IV P-type ATPases.
Data show that Lem3-Dnf1/Dnf2 complex regulates the polar Cdc42 dynamics and phosphatidylserine distribution.
Title: Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity.
aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae
Title: The putative aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae.
The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.
Internalization of Dnf1p from the plasma membrane uses an NPFXD endocytosis signal and its recognition by Sla1p.
Title: Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled intramembrane lipid transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATPase-coupled intramembrane lipid transporter activity	IEA Inferred from Electronic Annotation more info
enables glycosylceramide flippase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylcholine flippase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables phosphatidylcholine flippase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylcholine floppase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylethanolamine flippase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylethanolamine flippase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables phosphatidylethanolamine flippase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphatidylserine flippase activity	IEA Inferred from Electronic Annotation more info
enables phosphatidylserine floppase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in aminophospholipid translocation	IEA Inferred from Electronic Annotation more info
involved_in ceramide translocation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in intracellular protein transport	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lipid transport	IEA Inferred from Electronic Annotation more info
involved_in phospholipid translocation	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipid translocation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phospholipid translocation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
located_in cell periphery	HDA more info	PubMed
located_in cell septum	IEA Inferred from Electronic Annotation more info
located_in cellular bud	IEA Inferred from Electronic Annotation more info
located_in cellular bud neck	HDA more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in mating projection tip membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
part_of phospholipid-translocating ATPase complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: aminophospholipid-translocating P4-type ATPase DNF1

NP_011093.3

EC 7.6.2.1

Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

512744..517459

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179056.3 → NP_011093.3 TPA: aminophospholipid-translocating P4-type ATPase DNF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011093.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DM74, P32660

UniProtKB/TrEMBL

C8Z7I9, N1P787

Conserved Domains (1) summary

TIGR01652
Location:191 → 1413

ATPase-Plipid; phospholipid-translocating P-type ATPase, flippase