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MED14 mediator complex subunit 14 [ Homo sapiens (human) ]

Gene ID: 9282, updated on 27-Nov-2024

Summary

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Official Symbol
MED14provided by HGNC
Official Full Name
mediator complex subunit 14provided by HGNC
Primary source
HGNC:HGNC:2370
See related
Ensembl:ENSG00000180182 MIM:300182; AllianceGenome:HGNC:2370
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
Summary
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in thyroid (RPKM 8.7), duodenum (RPKM 8.0) and 25 other tissues See more
Orthologs
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Genomic context

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See MED14 in Genome Data Viewer
Location:
Xp11.4
Exon count:
33
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) X NC_000023.11 (40648305..40736159, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) X NC_060947.1 (40049523..40137378, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) X NC_000023.10 (40507557..40594794, complement)

Chromosome X - NC_000023.11Genomic Context describing neighboring genes Neighboring gene chromosome X open reading frame 38 Neighboring gene mitochondrial pyruvate carrier 1 like Neighboring gene Sharpr-MPRA regulatory region 4034 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29548 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29549 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20764 Neighboring gene ReSE screen-validated silencer GRCh37_chrX:40506849-40507029 Neighboring gene ReSE screen-validated silencer GRCh37_chrX:40535358-40535509 Neighboring gene ReSE screen-validated silencer GRCh37_chrX:40537853-40538063 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 20766 Neighboring gene H3K27ac hESC enhancer GRCh37_chrX:40594164-40595085 Neighboring gene H3K27ac hESC enhancer GRCh37_chrX:40595086-40596006 Neighboring gene MED14 opposite strand Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29550 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 29551 Neighboring gene CLDN7 pseudogene 1 Neighboring gene divergent-paired related homeobox pseudogene 6

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method. Yoshikawa H, et al. Genomics, 1998 Apr 15. PMID 9598311
  2. Coactivation of estrogen receptor alpha (ER alpha)/Sp1 by vitamin D receptor interacting protein 150 (DRIP150). Lee J, et al. Arch Biochem Biophys, 2007 May 15. PMID 17306756, Free PMC Article
  3. Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit. Cevher MA, et al. Nat Struct Mol Biol, 2014 Dec. PMID 25383669, Free PMC Article
  4. Refinement of the physical location and the genomic characterization of the CRSP2 (EXLM1) gene on Xp11.4. Demirci FY, et al. DNA Seq, 2003 Apr. PMID 12825353
  5. Vitamin D enhances glucocorticoid action in human monocytes: involvement of granulocyte-macrophage colony-stimulating factor and mediator complex subunit 14. Zhang Y, et al. J Biol Chem, 2013 May 17. PMID 23572530, Free PMC Article

See all (116) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Functional analysis implicates TNRC6A, NAT10, MED14, and WDR5 in RNA-mediated transcriptional activation.
    Title: Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
  2. This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II.
    Title: Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.
  3. VitD-mediated stimulation of GC anti-inflammatory affects human monocytes in a process involving GM-CSF and MED14
    Title: Vitamin D enhances glucocorticoid action in human monocytes: involvement of granulocyte-macrophage colony-stimulating factor and mediator complex subunit 14.
  4. MED14 and MED1 are used by glucocorticoid receptor in a gene-specific manner, providing a mechanism for promoter selectivity by glucocorticoid receptor
    Title: MED14 and MED1 differentially regulate target-specific gene activation by the glucocorticoid receptor.
  5. CRSP2 gene is expressed in the retina and its exact genomic location is on Xp11.4 between DXS1368 and DXS993
    Title: Refinement of the physical location and the genomic characterization of the CRSP2 (EXLM1) gene on Xp11.4.
  6. Coactivation of ERalpha by DRIP150 in ZR-75 cells is NR box-independent and requires a novel sequence with putative alpha-helical structure.
    Title: DRIP150 coactivation of estrogen receptor alpha in ZR-75 breast cancer cells is independent of LXXLL motifs.
  7. DRIP150 binds to ISGF3 and regulates transcription
    Title: Role of metazoan mediator proteins in interferon-responsive transcription.
  8. Coactivator that enhances estrogen receptor alpha- and specificity protein (SP)-1-mediated transactivation in breast cancer cells.
    Title: Coactivation of estrogen receptor alpha (ER alpha)/Sp1 by vitamin D receptor interacting protein 150 (DRIP150).
Submit: New GeneRIF Correction

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction Pubs
Knockdown of mediator complex subunit 14 (MED14) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed
Knockdown of mediator complex subunit 14 (MED14) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Protein interactions

Protein Gene Interaction Pubs
Tat tat SiRNA knockdown of MED14 shows a significant inhibition in HIV-1 Tat-mediated transcription levels as compared to control PubMed
tat The interaction of HIV-1 Tat with MED21 hypothetically induces the binding of Tat to MED14 PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC104513

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables nuclear receptor coactivator activity NAS
Non-traceable Author Statement
more info
 PubMed 
enables nuclear vitamin D receptor binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transcription coactivator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transcription coregulator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription coregulator activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA polymerase II preinitiation complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription elongation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in somatic stem cell population maintenance IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of core mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of core mediator complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mediator complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
mediator of RNA polymerase II transcription subunit 14
Names
ARC150
CRSP complex subunit 2
RGR1 homolog
activator-recruited cofactor 150 kDa component
cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)
hRGR1
human homolog of yeast RGR1
thyroid hormone receptor-associated protein complex 170 kDa component
thyroid hormone receptor-associated protein complex component TRAP170
transcriptional co-activator CRSP150
vitamin D receptor-interacting protein complex component DRIP150
vitamin D3 receptor-interacting protein complex 150 kDa component

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_016325.2 RefSeqGene

    Range
    5617..92854
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_004229.4 → NP_004220.2  mediator of RNA polymerase II transcription subunit 14

    See identical proteins and their annotated locations for NP_004220.2

    Status: REVIEWED

    Source sequence(s)
    AC093029, AF304448, BC098377
    Consensus CDS
    CCDS14254.1
    UniProtKB/Swiss-Prot
    O60244, Q4KMR7, Q9UNB3
    UniProtKB/TrEMBL
    A8KAK5
    Related
    ENSP00000323720.1, ENST00000324817.6
    Conserved Domains (2) summary
    pfam05109
    Location:1015 → 1189
    Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)
    pfam08638
    Location:51 → 238
    Med14; Mediator complex subunit MED14

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000023.11 Reference GRCh38.p14 Primary Assembly

    Range
    40648305..40736159 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047442636.1 → XP_047298592.1  mediator of RNA polymerase II transcription subunit 14 isoform X1

  2. XM_047442638.1 → XP_047298594.1  mediator of RNA polymerase II transcription subunit 14 isoform X3

  3. XM_047442643.1 → XP_047298599.1  mediator of RNA polymerase II transcription subunit 14 isoform X8

  4. XM_047442641.1 → XP_047298597.1  mediator of RNA polymerase II transcription subunit 14 isoform X6

  5. XM_047442640.1 → XP_047298596.1  mediator of RNA polymerase II transcription subunit 14 isoform X5

  6. XM_047442637.1 → XP_047298593.1  mediator of RNA polymerase II transcription subunit 14 isoform X2

  7. XM_047442642.1 → XP_047298598.1  mediator of RNA polymerase II transcription subunit 14 isoform X7

  8. XM_047442639.1 → XP_047298595.1  mediator of RNA polymerase II transcription subunit 14 isoform X4

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060947.1 Alternate T2T-CHM13v2.0

    Range
    40049523..40137378 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054328126.1 → XP_054184101.1  mediator of RNA polymerase II transcription subunit 14 isoform X1

  2. XM_054328128.1 → XP_054184103.1  mediator of RNA polymerase II transcription subunit 14 isoform X3

  3. XM_054328133.1 → XP_054184108.1  mediator of RNA polymerase II transcription subunit 14 isoform X8

  4. XM_054328131.1 → XP_054184106.1  mediator of RNA polymerase II transcription subunit 14 isoform X6

  5. XM_054328130.1 → XP_054184105.1  mediator of RNA polymerase II transcription subunit 14 isoform X5

  6. XM_054328127.1 → XP_054184102.1  mediator of RNA polymerase II transcription subunit 14 isoform X2

  7. XM_054328132.1 → XP_054184107.1  mediator of RNA polymerase II transcription subunit 14 isoform X7

  8. XM_054328129.1 → XP_054184104.1  mediator of RNA polymerase II transcription subunit 14 isoform X4

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O60244.2 GenPept UniProtKB/Swiss-Prot:O60244

Gene LinkOut

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