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dnaJ chaperone protein DnaJ [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944753, updated on 3-Dec-2024

Summary

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Official Symbol
dnaJ
Official Full Name
chaperone protein DnaJ
Primary source
ECOCYC:EG10240
Locus tag
b0015
See related
ASAP:ABE-0000054
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0015; groP; grpC
Summary
DnaK and DnaJ both bind to the same target peptide, forming a ternary complex. [More information is available at EcoGene: EG10240]. The DnaK system of Escherichia coli is a homolog of the eukaryotic Hsp70 chaperone system. [More information is available at EcoCyc: EG10240].
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Genomic context

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Sequence:
NC_000913.3 (14168..15298)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2541 domain-containing protein YaaI Neighboring gene chaperone protein DnaK Neighboring gene IS186/IS421 family transposase Neighboring gene protein HokC Neighboring gene regulatory protein MokC

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Genetic and biochemical characterization of mutations affecting the carboxy-terminal domain of the Escherichia coli molecular chaperone DnaJ. Goffin L, et al. Mol Microbiol, 1998 Oct. PMID 9791178
  2. Increased persistence in Escherichia coli caused by controlled expression of toxins or other unrelated proteins. Vázquez-Laslop N, et al. J Bacteriol, 2006 May. PMID 16672603, Free PMC Article
  3. The C-terminal (331-376) sequence of Escherichia coli DnaJ is essential for dimerization and chaperone activity: a small angle X-ray scattering study in solution. Shi YY, et al. J Biol Chem, 2005 Jun 17. PMID 15849180
  4. Contributions of cysteine residues in Zn2 to zinc fingers and thiol-disulfide oxidoreductase activities of chaperone DnaJ. Shi YY, et al. Biochemistry, 2005 Feb 8. PMID 15683252
  5. The roles of the two zinc binding sites in DnaJ. Linke K, et al. J Biol Chem, 2003 Nov 7. PMID 12941935

See all (159) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. In vitro Evolution of Uracil Glycosylase Towards DnaKJ and GroEL Binding Evolves Different Misfolded States.
    Title: In vitro Evolution of Uracil Glycosylase Towards DnaKJ and GroEL Binding Evolves Different Misfolded States.
  2. Potentiation of the activity of Escherichia coli chaperone DnaJ by tailing hyper-acidic minipeptides.
    Title: Potentiation of the activity of Escherichia coli chaperone DnaJ by tailing hyper-acidic minipeptides.
  3. BAH1 could partially compensate for the DnaJ' zinc finger domain in vivo, and that it was dependent on the zinc finger domain of BAH1.
    Title: BAH1 an E3 Ligase from Arabidopsis thaliana Stabilizes Heat Shock Factor σ(32) of Escherichia coli by Interacting with DnaK/DnaJ Chaperone Team.
  4. These results suggest that, in the absence of interaction with Escherichia coli DnaJ, the N-terminal domain of the MS2 bacteriophage L lysis protein interferes with its ability to bind to its unknown target.
    Title: MS2 Lysis of Escherichia coli Depends on Host Chaperone DnaJ.
  5. Fatty acid methyl esters analysis indicated that the amount of unsaturated fatty acid sharply increased and subcellular location prediction analysis showed a marked decrease in transcription of inner-membrane protein genes, which might have triggered the development of aberrant cell shape and susceptibility for some antibiotics in the DeltadnaKJ strain.
    Title: Large-scale gene expression profiling reveals physiological response to deletion of chaperone dnaKJ in Escherichia coli.
  6. The interaction of DnaK with DnaJ, GrpE, or sigma32 becomes weaker when DnaK is glutathionylated during oxidative stress, and the interaction is restored upon deglutathionylation.
    Title: Glutathionylation of the Bacterial Hsp70 Chaperone DnaK Provides a Link between Oxidative Stress and the Heat Shock Response.
  7. analysis of DnaJ-DnaK-GrpE chaperone cooperation
    Title: Action of the Hsp70 chaperone system observed with single proteins.
  8. DnaJ mutant is impaired in biofilm development when exposed to 42 degrees C for 2 h.
    Title: Chaperone DnaJ Influences the Formation of Biofilm by Escherichia coli.
  9. DnaJ-promoted binding of DnaK to multiple sites on sigma32 in the presence of ATP.
    Title: DnaJ-promoted binding of DnaK to multiple sites on σ32 in the presence of ATP.
  10. Trigger factor dependent refolding of bacterial luciferases in Escherichia coli cells: kinetics, efficiency and effect of the bichaperone system, DnaKJE-ClpB
    Title: [Trigger factor dependent refolding of bacterial luciferases in Escherichia coli cells: kinetics, efficiency and effect of the bichaperone system, DnaKJE-ClpB].
Submit: New GeneRIF Correction See all GeneRIFs (31)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables heat shock protein binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein disulfide isomerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-disulfide reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-folding chaperone binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sigma factor antagonist activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables unfolded protein binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables unfolded protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables unfolded protein binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in chaperone cofactor-dependent protein refolding IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within chaperone cofactor-dependent protein refolding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chaperone cofactor-dependent protein refolding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein folding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein folding IEA
Inferred from Electronic Annotation
more info
  
involved_in protein refolding IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within protein refolding IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein unfolding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein-containing complex assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to heat IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to heat IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to heat IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within viral process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within viral process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
chaperone protein DnaJ

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    14168..15298
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414556.1 chaperone protein DnaJ [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414556.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376MCT3
    Conserved Domains (1) summary
    PRK10767
    Location:1 → 372
    PRK10767; chaperone protein DnaJ; Provisional
Nucleotide Protein
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