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nhaA Na(+):H(+) antiporter NhaA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944758, updated on 3-Dec-2024

Summary

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Official Symbol
nhaA
Official Full Name
Na(+):H(+) antiporter NhaA
Primary source
ECOCYC:EG10652
Locus tag
b0019
See related
ASAP:ABE-0000068
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ant; antA; ECK0020
Summary
nhaA P2 promoter is RpoS-regulated, but H-NS, Na(+)- and NhaR-independent. [More information is available at EcoGene: EG10652]. NhaA is a sodium ion/proton antiporter that uses the proton electrochemical gradient to expel sodium ions from the cytoplasm and functions primarily in the adaptation to high salinity at alkaline pH. [More information is available at EcoCyc: EG10652].
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Genomic context

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Sequence:
NC_000913.3 (17489..18655)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein HokC Neighboring gene regulatory protein MokC Neighboring gene ncRNA Neighboring gene DNA-binding transcriptional activator NhaR Neighboring gene IS1 family protein InsB Neighboring gene IS1 family protein InsA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Controlled unfolding and refolding of a single sodium-proton antiporter using atomic force microscopy. Kedrov A, et al. J Mol Biol, 2004 Jul 23. PMID 15236973
  2. Functional role of polar amino acid residues in Na+/H+ exchangers. Wiebe CA, et al. Biochem J, 2001 Jul 1. PMID 11415429, Free PMC Article
  3. Screening of environmental DNA libraries for the presence of genes conferring Na(+)(Li(+))/H(+) antiporter activity on Escherichia coli: characterization of the recovered genes and the corresponding gene products. Majerník A, et al. J Bacteriol, 2001 Nov. PMID 11673435, Free PMC Article
  4. Molecular model of the Escherichia coli Na1/H1 antiporter NhaA. Ravna AW, et al. Recept Channels, 2001. PMID 11697236
  5. High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm. Venturi M, et al. J Mol Biol, 2002 Jan 4. PMID 11771962

See all (119) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study.
    Title: Site-directed mutagenesis at the Glu78 in Ec-NhaA transporter impacting ion exchange: a biophysical study.
  2. Towards Molecular Understanding of the pH Dependence Characterizing NhaA of Which Structural Fold is Shared by Other Transporters.
    Title: Towards Molecular Understanding of the pH Dependence Characterizing NhaA of Which Structural Fold is Shared by Other Transporters.
  3. An angular motion of a conserved four-helix bundle facilitates alternating access transport in the TtNapA and EcNhaA transporters.
    Title: An angular motion of a conserved four-helix bundle facilitates alternating access transport in the TtNapA and EcNhaA transporters.
  4. Alternative proton-binding site and long-distance coupling in Escherichia coli sodium-proton antiporter NhaA.
    Title: Alternative proton-binding site and long-distance coupling in Escherichia coli sodium-proton antiporter NhaA.
  5. findings indicate that alternative mechanisms sustain EcNhaA activity in the absence of canonical ion-binding residues and that the conserved lysines confer structural stability.
    Title: Replacement of Lys-300 with a glutamine in the NhaA Na(+)/H(+) antiporter of Escherichia coli yields a functional electrogenic transporter.
  6. analysis of NhaA pH-dependent activation by molecular dynamics simulation of atomic details of proton-coupled transport across membrane
    Title: Mechanism of pH-dependent activation of the sodium-proton antiporter NhaA.
  7. Lysine 300 has an essential role in stability but not electrogenic transport of Escherichia coli NhaA
    Title: Lysine 300 is essential for stability but not for electrogenic transport of the Escherichia coli NhaA Na(+)/H(+) antiporter.
  8. Data show that the Li(+) binding, H(+) release and antiporter activity were all affected to the same extent by Na(+), Li(+)/H(+) antiporter (NhaA) mutations in the Li(+) binding site (D163E, D163N, D164N, D164E).
    Title: The Ec-NhaA antiporter switches from antagonistic to synergistic antiport upon a single point mutation.
  9. Data show that the two aspartic acid residues of Na+/H+ antiporter NhaA, D163 and D164, act as Na+ trap and energetic barrier for the transport of Na+.
    Title: Simulating the function of sodium/proton antiporters.
  10. NhaA antiporter functions using 10 helices, and an additional 2 contribute to assembly/stability.
    Title: NhaA antiporter functions using 10 helices, and an additional 2 contribute to assembly/stability.
Submit: New GeneRIF Correction See all GeneRIFs (27)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables antiporter activity IEA
Inferred from Electronic Annotation
more info
  
enables cardiolipin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sodium ion transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables sodium:proton antiporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sodium:proton antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in regulation of intracellular pH TAS
Traceable Author Statement
more info
 PubMed 
involved_in regulation of pH IEA
Inferred from Electronic Annotation
more info
  
involved_in response to alkaline pH IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to alkaline pH IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to salt stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sodium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in sodium ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
Na(+):H(+) antiporter NhaA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    17489..18655
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414560.1 Na(+):H(+) antiporter NhaA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414560.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7CVL3, A0A8S7I7R4
    Conserved Domains (1) summary
    PRK09561
    Location:1 → 388
    nhaA; sodium/proton antiporter NhaA
Nucleotide Protein
Heading Accession and Version

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