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lpxA acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944849, updated on 3-Dec-2024

Summary

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Official Symbol
lpxA
Official Full Name
acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Primary source
ECOCYC:EG10545
Locus tag
b0181
See related
ASAP:ABE-0000618
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0180
Summary
LpxA contains 24 hexapeptide repeats. [More information is available at EcoGene: EG10545]. UDP-N-acetylglucosamine acyltransferase (LpxA) catalyzes the first reaction of lipid A biosynthesis. [More information is available at EcoCyc: EG10545].
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Genomic context

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Sequence:
NC_000913.3 (202560..203348)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase Neighboring gene 3-hydroxy-acyl-[acyl-carrier-protein] dehydratase Neighboring gene lipid A disaccharide synthase Neighboring gene RNase HII Neighboring gene DNA polymerase III subunit alpha

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The active site of Escherichia coli UDP-N-acetylglucosamine acyltransferase. Chemical modification and site-directed mutagenesis. Wyckoff TJ, et al. J Biol Chem, 1999 Sep 17. PMID 10480918
  2. The lipid A biosynthesis mutation lpxA2 of Escherichia coli results in drastic antibiotic supersusceptibility. Vuorio R, et al. Antimicrob Agents Chemother, 1992 Apr. PMID 1503445, Free PMC Article
  3. Acyl-acyl carrier protein specificity of UDP-GlcNAc acyltransferases from gram-negative bacteria: relationship to lipid A structure. Williamson JM, et al. J Bacteriol, 1991 Jun. PMID 1904441, Free PMC Article
  4. A mutant of Escherichia coli defective in the first step of endotoxin biosynthesis. Galloway SM, et al. J Biol Chem, 1990 Apr 15. PMID 2180947
  5. Biosynthesis of lipid A precursors in Escherichia coli. A cytoplasmic acyltransferase that converts UDP-N-acetylglucosamine to UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine. Anderson MS, et al. J Biol Chem, 1987 Apr 15. PMID 3549716

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Overlay of the LpxA-RJPXD33 structure with E. coli LpxD identified a complementary peptide binding pocket within LpxD and serves as a model for further biochemical characterization of RJPXD33 binding to LpxD.
    Title: Structural basis for the recognition of peptide RJPXD33 by acyltransferases in lipid A biosynthesis.
  2. N-terminus verified by Edman degradation on complete protein
    Title: UDP-N-acetylglucosamine acyltransferase of Escherichia coli. The first step of endotoxin biosynthesis is thermodynamically unfavorable.
  3. show how LpxA selects for 14-carbon R-3-hydroxyacyl chains and reveal two modes of UDP binding
    Title: Structural basis for the acyl chain selectivity and mechanism of UDP-N-acetylglucosamine acyltransferase.
  4. Results suggest that nucleotide substrate recognition by UDP-N-acetylglucosamine acyltransferase (LpxA) in the first step of lipid A biosynthesis.
    Title: Nucleotide substrate recognition by UDP-N-acetylglucosamine acyltransferase (LpxA) in the first step of lipid A biosynthesis.
  5. compact beta-turn structure of peptide 920 bound to LpxA may open previously uncharacterized approaches to the rational design of LpxA inhibitors with antibiotic activity
    Title: Structure of UDP-N-acetylglucosamine acyltransferase with a bound antibacterial pentadecapeptide.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables acyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables acyltransferase activity, transferring groups other than amino-acyl groups IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in lipid A biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
NP_414723.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    202560..203348
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414723.1 acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414723.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AM74, Q0P6M6
    Conserved Domains (1) summary
    PRK05289
    Location:4 → 262
    PRK05289; acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase
Nucleotide Protein
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