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tilS tRNA(Ile)-lysidine synthetase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944889, updated on 3-Dec-2024

Summary

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Official Symbol
tilS
Official Full Name
tRNA(Ile)-lysidine synthetase
Primary source
ECOCYC:G6096
Locus tag
b0188
See related
ASAP:ABE-0000638
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0187; mesJ; yaeN
Summary
YaeN (TilS) is mistakenly labeled as a cell cycle gene in many databases. the confusion arose from the misinterpretation of Genbank Z50870. the real "minE suppressor" is the small rof gene next to tilS, labeled as orf4 in Z50870. [More information is available at EcoGene: EG13220]. TilS is a tRNAIle-lysidine synthetase, the enzyme responsible for modifying the wobble-base of the CAU anticodon of tRNAIle such that it exhibits proper recognition of the AUA codon rather than the AUG codon . [More information is available at EcoCyc: G6096].
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Genomic context

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Sequence:
NC_000913.3 (212331..213629)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene lysine decarboxylase 2 Neighboring gene VOC domain-containing protein YaeR Neighboring gene modulator of Rho-dependent transcription termination Neighboring gene PF06786 family protein YaeP Neighboring gene uncharacterized protein YaeQ Neighboring gene peptidyl-tRNA hydrolase, ribosome rescue factor

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Discovery and characterization of tRNAIle lysidine synthetase (TilS). Suzuki T, et al. FEBS Lett, 2010 Jan 21. PMID 19944692
  2. Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase. Nakanishi K, et al. Nature, 2009 Oct 22. PMID 19847269
  3. Differential annotation of tRNA genes with anticodon CAT in bacterial genomes. Silva FJ, et al. Nucleic Acids Res, 2006. PMID 17071718, Free PMC Article
  4. molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition. Ikeuchi Y, et al. Mol Cell, 2005 Jul 22. PMID 16039592
  5. Enzymatic conversion of cytidine to lysidine in anticodon of bacterial isoleucyl-tRNA--an alternative way of RNA editing. Grosjean H, et al. Trends Biochem Sci, 2004 Apr. PMID 15124629

See all (18) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity, forming carbon-nitrogen bonds IEA
Inferred from Electronic Annotation
more info
  
enables tRNA(Ile)-lysidine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA(Ile)-lysidine synthase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within tRNA modification IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA modification IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA wobble base lysidine biosynthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
tRNA(Ile)-lysidine synthetase
NP_414730.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    212331..213629
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414730.1 tRNA(Ile)-lysidine synthetase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414730.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6N8NGM9
    Conserved Domains (1) summary
    PRK10660
    Location:1 → 432
    tilS; tRNA(Ile)-lysidine synthetase; Provisional
Nucleotide Protein
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